{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:32:45Z","timestamp":1790620365127,"version":"4.1.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1975,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n        <jats:p>In order to characterize the uterine progesterone-binding proteins of oestrogen-primed and unprimed, ovariectomized immature and adult golden hamsters, cytosols were incubated with [<jats:sup>3<\/jats:sup>H] progesterone and\/or other steroids and analyzed by sucrose-glycerol density gradient ultracentrifugation. Progesterone-binding components with sedimentation coefficients of 7S and 4.5S were found in the uterine cytosols, but not in the cytosols from the hypothalamus, pituitary, diaphragm, or small intestine. Oestradiol-17\u03b2 markedly elevated the level of 7S uterine receptor and this increase appeared to be due to new receptor synthesis, since actinomycin D and cycloheximide blocked this response. Fifty to 100 \u03bcg of oestradiol-17\u03b2 per kg body weight was found to promote a maximum increase in the 7S macromolecular component. The 7S receptor showed a tendency to saturate at 1 \u00d7 10<jats:sup>\u22127<\/jats:sup> <jats:sc>m<\/jats:sc> [1,2-<jats:sup>3<\/jats:sup>H] progesterone, indicating limited capacity. At a molar ratio of 100:1, unlabelled progesterone competed effectively for 7S and 4.5S [<jats:sup>3<\/jats:sup>H]progesterone binding, whereas 5\u03b1-pregnane-3,20-dione, oestradiol-17\u03b2 and testosterone did not. Moreover, [1,2-<jats:sup>3<\/jats:sup>H] cortisol and [1,2-<jats:sup>3<\/jats:sup>H] corticosterone did not bind to the 7S receptor, implying steroid specificity. CI-628, a non-steroid oestrogen antagonist, completely prevented [6,7-<jats:sup>3<\/jats:sup>H] oestradiol-17\u03b2 binding to its 8.5S uterine cytosol receptor, but was entirely without effect on 7S and 4.5S [<jats:sup>3<\/jats:sup>H] progesterone binding. Pronase, but not DNase or RNase, abolished 7S and 4.5S progesterone binding, suggesting that the binders are at least in part protein. Protamine sulphate and p-hydroxymercuribenzoate also obliterated 7S binding, implying that this receptor may be an acidic protein which contains sulfhydryl groups that are necessary for progesterone binding.<\/jats:p>","DOI":"10.1530\/acta.0.0800344","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T21:31:09Z","timestamp":1229376669000},"page":"344-354","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":13,"title":["OESTROGEN-INDUCIBLE UTERINE PROGESTERONE RECEPTORS. CHARACTERISTICS IN THE OVARIECTOMIZED IMMATURE AND ADULT HAMSTER"],"prefix":"10.1093","volume":"80","author":[{"given":"J. R.","family":"Reel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Shih","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/80\/2\/acta_80_2_014.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/80\/2\/acta_80_2_014.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:25:40Z","timestamp":1676391940000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/80\/2\/344\/6839004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,10]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.0800344","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1975,10]]}}}