{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:38:53Z","timestamp":1760204333000},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1977,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n<jats:p>A cloned cell line of human choriocarcinoma was evaluated as a model of human placental oestrogen production. Oestrone formation from dehydroepiandrosterone (D), D-sulphate (DS) or 4-androstenedione (A) was \u2266 5% of oestradiol-17\u03b2 (Oe<jats:sub>2<\/jats:sub>) formation. Oe<jats:sub>2<\/jats:sub> formation from D and A was similar (100\u2013150 pmole\/h\/10<jats:sup>7<\/jats:sup> cells); that from DS was 10 times less. Omitting serum from the medium increased Oe<jats:sub>2<\/jats:sub> yield from DS 4-fold; addition of albumin restored these yields to control values (<jats:italic>P<\/jats:italic> &gt; 0.05, <jats:italic>t<\/jats:italic>-test), presumably by binding DS. N<jats:sup>6<\/jats:sup>,0<jats:sup>2<\/jats:sup>\u2032-dibutyryl-adenosine 3\u2032,5\u2032-cyclic monophosphoric acid and theophylline treatment for 72 h stimulated (<jats:italic>P<\/jats:italic> &lt;0.01) Oe<jats:sub>2<\/jats:sub> formation from D (36 %), DS (66%) and A (183 %). In intact cells, sulphatase activity, Oe<jats:sub>2<\/jats:sub> formation from D and Oe<jats:sub>2<\/jats:sub> formation from DS equalled those in homogenates (<jats:italic>P<\/jats:italic> &gt; 0.05) but Oe<jats:sub>2<\/jats:sub> formation from D was greater than that from DS in both systems (<jats:italic>P<\/jats:italic> &lt; 0.001), indicating a deficiency of sulphatase relative to subsequent enzymes of oestrogen synthesis. Steroids, at concentrations previously shown to inhibit placental sulphatase or 3\u03b2-hydroxysteroid dehydrogenase, did not inhibit choriocarcinoma enzymes. Except for its relative sulphatase deficiency and insusceptibility of oestrogen synthesizing enzymes to steroid inhibitors, choriocarcinoma appears to be a useful model of placental oestrogen synthesis.<\/jats:p>","DOI":"10.1530\/acta.0.0840633","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T21:31:09Z","timestamp":1229376669000},"page":"633-641","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":3,"title":["OESTROGEN FORMATION FROM C19 PRECURSORS IN HUMAN CHORIOCARCINOMA IN CULTURE"],"prefix":"10.1093","volume":"84","author":[{"given":"Herman A. J.","family":"Schut","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D.","family":"Townsley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/84\/3\/acta_84_3_019.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/84\/3\/acta_84_3_019.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:28:42Z","timestamp":1676392122000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/84\/3\/633\/6840193"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,3]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.0840633","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1977,3]]}}}