{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T23:10:46Z","timestamp":1678835446357},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1977,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n<jats:p>Thyroxine (T<jats:sub>4<\/jats:sub>) and triiodothyronine (T<jats:sub>3<\/jats:sub>)-binding interactions in preparations of rat anterior pituitary gland have been studied. T<jats:sub>4<\/jats:sub> is bound primarily to extranuclear binding sites located in the cytosol fraction of the cell. These sites have a medium affinity for T<jats:sub>4<\/jats:sub>: Ka = 2.5 \u00d7 10<jats:sup>8<\/jats:sup> 1\/mol and a maximum binding capacity (MBC) of 1.15 pmol\/mg tissue (wet weight). Binding of T<jats:sub>3<\/jats:sub> to these sites is minimal. The extent of binding of T<jats:sub>4<\/jats:sub> is influenced by the pH of the system and the temperature of incubation. The relative effectiveness of T<jats:sub>4<\/jats:sub> analogues in displacing bound T<jats:sub>4<\/jats:sub> is tetrac &gt; T<jats:sub>4<\/jats:sub> &gt; triac &gt; D-T<jats:sub>4<\/jats:sub> &gt; T<jats:sub>3<\/jats:sub>. Similar T<jats:sub>4<\/jats:sub>-binding sites are present in other rat tissues, but in all except serum, binding activity is lower than in the pituitary. T<jats:sub>4<\/jats:sub>-binding by serum contaminating the pituitary preparations contributed only partially to the total activity observed. Concomitant assessment of T<jats:sub>4<\/jats:sub>-binding activity and T<jats:sub>4<\/jats:sub> metabolism in pituitary homogenates prepared at different pH values indicated an inverse relationship between the two processes. The possible role of thyroid hormone binding in cytosol in influencing the intracellular distribution of thyroid hormones is discussed.<\/jats:p>","DOI":"10.1530\/acta.0.0850256","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T21:31:09Z","timestamp":1229376669000},"page":"256-266","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":2,"title":["THYROID HORMONE-BINDING INTERACTIONS IN CYTOSOL OF RAT ANTERIOR PITUITARY"],"prefix":"10.1093","volume":"85","author":[{"given":"Valerie Anne","family":"Galton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/85\/2\/acta_85_2_004.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/85\/2\/acta_85_2_004.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:28:59Z","timestamp":1676392139000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/85\/2\/256\/6842046"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,6]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.0850256","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1977,6]]}}}