{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:14:22Z","timestamp":1760044462146},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1978,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n<jats:p>Specific antisera against tetrahydroaldosterone (TH-Aldo) were raised in two white New Zealand rabbits. 3\u03b1,5\u03b2-TH-aldo-20-oxime-bovine-serum albumin complex was used as antigen. The resulting titers were 1:18 000 and 1:16 000. Except tetrahydrocortisol (THF) (0.23%) and tetrahydro-18-hydroxy-11-dehydrocorticosterone (18-OH-THA) (3.2 %), all steroids and steroid metabolites gave negligible cross-reactions. Immunograms of the paper chromatograms made from the n-butanol-extract of the urines, as well as after \u03b2-glucuronidase treatment and dichlormethane extraction, were studied to further define the specificity of the antiserum. Antibody H1 (used in this study) reacted with aldosterone-18-gluc., a TH-aldosterone-glucuronide (probably the 21-glucuronide) and an unidentified less polar material.<\/jats:p>\n<jats:p>Two methods were developed:<\/jats:p>\n<jats:p>a) TH-Aldo-glucuronidc(s) estimation after ethylacetate pre-extraction as a rapid screening test of endogenous aldosterone production.<\/jats:p>\n<jats:p>b) estimation of TH-aldosterone using one chromatographic system.<\/jats:p>\n<jats:p>The results of method a) showed a significant correlation with the values obtained by technique b). Normal values (method b) were 25.88 \u00b1 16.50 \u03bcg\/24h (range 9.5\u201364.8 \u03bcg\/24h).<\/jats:p>\n<jats:p>A significant correlation was also shown between the TH-aldo (technique b) and 18-gluc. values.<\/jats:p>","DOI":"10.1530\/acta.0.0870596","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T21:31:09Z","timestamp":1229376669000},"page":"596-608","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":10,"title":["RADIOIMMUNOASSAYS OF TETRAHYDROALDOSTERONE (TH-ALDO) IN HUMAN URINE"],"prefix":"10.1093","volume":"87","author":[{"given":"K.-H.","family":"Kohl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Vecsei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Abdelhamid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/87\/3\/acta_87_3_017.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/87\/3\/acta_87_3_017.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:30:15Z","timestamp":1676392215000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/87\/3\/596\/6802717"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,3]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.0870596","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,3]]}}}