{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T05:23:50Z","timestamp":1676438630796},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1979,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n        <jats:p>In order to characterize more accurately the relationship between immunoreactive \u03b2-MSH (\"\u03b2-MSH\") and the lipotrophins (LPH) we attempted to investigate the gel filtration and the immunological characteristics of \"\u03b2-MSH\" in the plasma of patients with Nelson's syndrome and Addison's disease as well as in the culture medium from a human corticotrophic adenoma using a sensitive radioimmunoassay from human \u03b2-MSH.<\/jats:p>\n        <jats:p>When added either to hormone free plasma or to a plasma from a patient with Nelson's syndrome all the human \u03b2-MSH (h\u03b2-MSH) elutes from a Sephadex G-50 column as a single peak in a volume corresponding to its molecular weight. In contrast plasma \"\u03b2-MSH\" in 3 patients with Nelson's syndrome and one patient with Addison's disease almost completely elutes in a volume corresponding to a molecular weight range of 6000\u2013 10 000; no \"\u03b2-MSH\" can be detected in its normal elution volume. Drastic pH change (8.2 to 2.3) does not significantly alter the elution pattern. Chromatography of a corticotrophic adenoma culture medium gave a similar pattern of \"\u03b2-MSH\" with a main peak in the molecular weight range of 6000\u201310 000. In our radioimmunoassay the culture medium and purified h\u03b2-LPH gave parallel displacement curves for [<jats:sup>125<\/jats:sup>I]h\u03b2-MSH. It is suggested that h\u03b2-LPH or a closely related substance is the main material responsible for \"\u03b2-MSH\" immunoactivity.<\/jats:p>","DOI":"10.1530\/acta.0.0900008","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T21:36:12Z","timestamp":1229376972000},"page":"8-17","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":0,"title":["IMMUNOREACTIVE \u03b2-MSH IN HUMAN PLASMA AND IN A CORTICOTROPHIC ADENOMA CULTURE MEDIUM. ITS RELATION TO THE LIPOTROPHINS"],"prefix":"10.1093","volume":"90","author":[{"given":"X.","family":"Bertagna","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Donnadieu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Seurin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Binoux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Girard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/90\/1\/acta_90_1_002.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/90\/1\/acta_90_1_002.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:31:23Z","timestamp":1676392283000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/90\/1\/8\/6803141"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,1]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.0900008","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,1]]}}}