{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T01:37:59Z","timestamp":1759541879454},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1980,1]]},"abstract":"<jats:title>Abstract.<\/jats:title>\n<jats:p>In vivo and in vitro effects of ethanol on the kinetics of insulin secretion in response to glucose and tolbutamide were studied in perifused rat islets. Phases I and II insulin response to 16.7 m<jats:sc>m<\/jats:sc> glucose was decreased 46% and 48%, respectively, in islets of rats given ethanol intragastrically 1 g\/kg 1 h prior to sacrifice. Mean blood ethanol levels at the time of animal sacrifice were 19.4 mmol\/l. The magnitude of insulin suppression was not significantly enhanced with higher ethanol doses, 2 or 3 g\/kg, although mean blood ethanol levels increased to 25.9 and 60.3 mmol\/l, respectively. Similarly, significant inhibition of both phases of insulin response to glucose occurred when ethanol 1 or 3 g\/kg was given intraperitoneally instead of orally. Ethanol had no effect on insulin secretion when given orally 4 h instead of 1 h prior to islet isolation.<\/jats:p>\n<jats:p>Ethanol, 65 mmol\/l, added directly to rat islets perifusate simultaneously with 16.7 m <jats:sc>m<\/jats:sc> glucose decreased both phases I and II insulin response nearly half; whereas addition of 21.7 instead of 65 mmol\/l ethanol had no effect. Pre-treatment of islets with 21.7 or 65 mmol\/l ethanol during 30 min basal islets perifusion period had no effect on subsequent insulin response to 16.7 m<jats:sc>m<\/jats:sc> glucose. Insulin response to 10 m<jats:sc>m<\/jats:sc> tolbutamide was decreased nearly 81% by the simultaneous presence of 65 mmol\/l ethanol in islets perifusate.<\/jats:p>","DOI":"10.1530\/acta.0.0930061","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T21:36:12Z","timestamp":1229376972000},"page":"61-66","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":8,"title":["Ethanol inhibition of insulin secretion by perifused rat islets"],"prefix":"10.1093","volume":"93","author":[{"given":"Sant P.","family":"Singh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. G.","family":"Patel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann K.","family":"Snyder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/93\/1\/acta_93_1_011.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/93\/1\/acta_93_1_011.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:33:17Z","timestamp":1676392397000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/93\/1\/61\/6803516"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,1]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.0930061","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,1]]}}}