{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T01:13:35Z","timestamp":1773278015055,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1981,6]]},"abstract":"<jats:title>Abstract.<\/jats:title>\n<jats:p>A radioimmunoassay (RIA) for serum 3,5-diiodothyronine (3,5-T<jats:sub>2<\/jats:sub>) was developed using small Sephadex G 25 (fine) columns. Prior to the RIA an alcohol extraction of 3,5-T<jats:sub>2<\/jats:sub> from serum and an evaporation of the extract was performed. The recovery of 3,5-T<jats:sub>2<\/jats:sub> added to serum was in mean (\u00b1 <jats:sc>sem<\/jats:sc>) 101 \u00b1 11%. The lower detection limit was 0.012 pmol\/column corresponding to 14 pmol\/l using 3 ml serum. Due to a 5% cross-reaction of the 3,5-T2 antibody with 3,5,3'-triiodothyronine (T<jats:sub>3<\/jats:sub>) individual correction for T<jats:sub>3<\/jats:sub> present in serum was necessary. Serum 3,5-T<jats:sub>2<\/jats:sub> levels in 52 \u00e9uthyroid controls were (mean \u00b1 <jats:sc>SD<\/jats:sc>) 105 \u00b1 51 pmol\/l. Serum levels were higher in men (125 \u00b1 56 pmol\/l) than in women (84 \u00b1 34 pmol\/ l, <jats:italic>P<\/jats:italic> &lt; 0.005). In 17 hyperthyroid subjects serum 3,5-T<jats:sub>2<\/jats:sub> levels were elevated (232 \u00b1 187 pmol\/l, <jats:italic>P<\/jats:italic> &lt; 0.005). In hypothyroid patients and patients with non-toxic goitre serum 3,5-T<jats:sub>2<\/jats:sub> levels did not differ significantly from control values, whereas 8 patients with liver cirrhosis had severely reduced serum 3,5-T<jats:sub>2<\/jats:sub> concentrations (20 \u00b1 23 pmol\/l, <jats:italic>P<\/jats:italic> &lt; 0.001).<\/jats:p>","DOI":"10.1530\/acta.0.0970196","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T21:36:12Z","timestamp":1229376972000},"page":"196-201","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":3,"title":["A radioimmunoassay of serum 3,5-diiodothyronine"],"prefix":"10.1093","volume":"97","author":[{"given":"Carsten","family":"Kirkegaard","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens","family":"Faber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaj","family":"Siersb\u00e6k-Nielsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thorkild","family":"Friis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/97\/2\/acta_97_2_008.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/97\/2\/acta_97_2_008.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:11:07Z","timestamp":1676391067000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/97\/2\/196\/6804068"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,6]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.0970196","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,6]]}}}