{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T14:33:33Z","timestamp":1756996413404},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1981,11]]},"abstract":"<jats:title>Abstract.<\/jats:title>\n<jats:p>The in vitro stimulation of [<jats:sup>3<\/jats:sup>H]thymidine uptake into lectin-activated lymphocytes in the presence of various sera was studied. The mean precision of the assay is 5%, and the study of the confidence intervals shows variations from 4% to 12%. Compared to a normal reference serum (fixed as 1 U\/ml), the serum thymidine uptake stimulating activity (mean \u00b1 <jats:sc>sem<\/jats:sc>) was 1.04 \u00b1 0.07 U\/ml in normal adult males, 2.63 \u00b1 0.48 U\/ml in acromegalic patients, 1.51 \u00b1 0.13 U\/ml in constitutional dwarfism and 0.37 \u00b1 0.04 U\/ml in untreated hypopituitary dwarfism with a significant difference between the groups (<jats:italic>P<\/jats:italic> &lt; 0.001). In patients with hypopituitarism a single im hGH dose (6 mg\/m<jats:sup>2<\/jats:sup>) increased the thymidine uptake stimulating activity of serum within 24 to 48 h following injection. The effects of directly adding hGH, insulin and T<jats:sub>3<\/jats:sub> to the assay, have been studied: pharmacological concentrations are required to produce only a slight effect. Physiological concentration of a purified preparation of somatomedin A stimulated thymidine uptake and its effect is increased in the presence of serum. These data demonstrate that [<jats:sup>3<\/jats:sup>H]thymidine uptake into lectin-activated lymphocytes is stimulated by a GH-dependent serum factor. The data suggest that this method should be proposed for an accurate and sensitive biological evaluation of serum thymidine uptake stimulating activity.<\/jats:p>","DOI":"10.1530\/acta.0.0980358","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T21:36:12Z","timestamp":1229376972000},"page":"358-363","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":3,"title":["A hormonally controlled serum factor stimulating the thymidine uptake into lectin-activated lymphocytes"],"prefix":"10.1093","volume":"98","author":[{"given":"Genevi\u00e8ve","family":"Thieriot-Pr\u00e9vost","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rose-Marie","family":"Schimpff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Sharif","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/98\/3\/acta_98_3_007.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/98\/3\/acta_98_3_007.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:12:05Z","timestamp":1676391125000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/98\/3\/358\/6803980"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,11]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.0980358","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,11]]}}}