{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:12:26Z","timestamp":1790673146408,"version":"4.1.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1982,5]]},"abstract":"<jats:title>Abstract.<\/jats:title>\n<jats:p>Highly specific antibodies to 13, 14-dihydro-15-ketoprostaglandin F<jats:sub>2\u03b1<\/jats:sub> (PGFM) were raised in rabbits. The animals were immunized with PGFM-bovine serum albumin (BSA)-conjugates.<\/jats:p>\n<jats:p>Prior to the incubation procedure PGFM was extracted by a rapid method with dichloromethane followed by column chromatography.<\/jats:p>\n<jats:p>The antisera dilution was 1:10000 and the cross-reactivity towards prostaglandin A<jats:sub>2<\/jats:sub>, E<jats:sub>2<\/jats:sub>, F<jats:sub>2\u03b1<\/jats:sub>, 13, 14-dihydro-15-ketoprostaglandin E<jats:sub>2<\/jats:sub> and the 15-ketoprostaglandin E<jats:sub>2<\/jats:sub> and F<jats:sub>2\u03b1<\/jats:sub> was &lt; 1%. The limit of detection was 1.9 \u00b1 0.6 pg\/ml plasma over the standard range 1.9\u2013250 pg. The intra- and inter-assay variations were 3.9 and 15%, respectively.<\/jats:p>\n<jats:p>PGFM was measured throughout the menstrual cycle in female volunteers.<\/jats:p>\n<jats:p>In normal ovulatory women (n = 3) plasma levels of PGFM varied between 65.6 to 107.1 pg\/ml.<\/jats:p>\n<jats:p>No significant variations of plasma PGFM were seen during the cycle. In anovulatory women (n = 4) no difference of PGFM was found during the cycle. PGFM levels in hyperprolactinaemic but ovulating women tend to be higher than in anovulatory, and normoprolactinaemic subjects.<\/jats:p>\n<jats:p>These data strongly indicate that PGFM is not correlated with other hormonal parameters tested here in the normal and anovulatory cycles.<\/jats:p>","DOI":"10.1530\/acta.0.1000098","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T21:36:12Z","timestamp":1229376972000},"page":"98-104","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":8,"title":["Radioimmunoassay for 13, 14-dihydro-15-ketoprostaglandin F2\u03b1 and its application in normo- and anovulatory women"],"prefix":"10.1093","volume":"100","author":[{"given":"Werner","family":"Schlegel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Urdinola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann P. G.","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/100\/1\/acta_100_1_016.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/100\/1\/acta_100_1_016.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:13:24Z","timestamp":1676391204000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/100\/1\/98\/6803060"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,5]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.1000098","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,5]]}}}