{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T01:37:46Z","timestamp":1759541866810},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1984,5]]},"abstract":"<jats:p>Abstract. Dispersed corticotrophic cells of 3 patients with Nelson's syndrome were studied in tissue culture for up to 25 days. During this culture period a parallel decrease with time was seen in ACTH and \u03b2-endorphin-like immunoreactivity (LIR) release. A concomitant decline was observed for intracellular hormones. The time course of hormone release showed a parallel secretion of ACTH and \u03b2-endorphin-LIR up to 8 h.<\/jats:p>\n          <jats:p>Both the release of ACTH and \u03b2-endorphin LIR were stimulated by 0.1 \u03bc<jats:sc>m<\/jats:sc> lysine vasopressin (LVP) in all three adenoma cell cultures. Dexamethasone (0.1 and 1 \u03bc<jats:sc>m<\/jats:sc>) suppressed basal hormone secretion for 4 h. Synthetic ovine corticotrophin-releasing factor (CRF) at 10 and 100 n<jats:sc>m<\/jats:sc> stimulated the secretion of ACTH and \u03b2-endorphin LIR maximally. This stimulation was higher than observed with maximal stimulative concentration of LVP (0.3 \u03bc<jats:sc>m<\/jats:sc>). The CRF-mediated hormone secretion was calcium-dependent. Dexamethasone (0.1 \u03bc<jats:sc>m<\/jats:sc>) blocked the stimulating effect of 10 n<jats:sc>m<\/jats:sc> CRF completely.<\/jats:p>\n          <jats:p>Gel-filtration chromatography demonstrated the cells to secrete both \u03b2-lipotrophin (\u03b2-LPH) and \u03b2-endorphin. The ratio of \u03b2-LPH to \u03b2-endorphin released remained constant upon stimulation by LVP and CRF. HPLC studies demonstrate the possibility that several \u03b2-endorphin fragments, including \u03b1-endorphin and \u03b3-endorphin, were secreted by cells from a Nelson tumour. CRF caused a simultaneous parallel stimulation of the release of these peptides.<\/jats:p>","DOI":"10.1530\/acta.0.1060021","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T21:53:47Z","timestamp":1229378027000},"page":"21-29","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":5,"title":["ACTH and \u03b2-endorphin secretion by three corticotrophic adenomas in culture. Effects of culture time, dexamethasone, vasopressin and synthetic corticotrophin releasing factor"],"prefix":"10.1093","volume":"106","author":[{"given":"R.","family":"Oosterom","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Verleun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Uitterlinden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. H. L.","family":"Hackeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. P. H.","family":"Burbach","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. M.","family":"Wiegant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. W. J.","family":"Lamberts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/106\/1\/acta_106_1_004.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/106\/1\/acta_106_1_004.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:18:10Z","timestamp":1676391490000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/106\/1\/21\/6801617"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,5]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.1060021","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,5]]}}}