{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T05:25:12Z","timestamp":1676438712314},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:p>Abstract. In order to obtain a better resolution of circulating PTH-fragments, we developed a radioimmunoassay with an antiserum raised against the synthetic 70\u201384 hPTH molecule in a sheep. [<jats:sup>125<\/jats:sup>I](69 Tyr) 70\u201384 hPTH was used as the radioactive tracer with the antiserum at a final dilution of 1:200 000. Displacement curves showed that only the 70\u201384 hPTH, (69 Tyr) 70\u201384 hPTH and 53\u201384 hPTH fragments were able to compete with the tracer. Synthetic 28\u201348 hPTH, 44\u201368 hPTH, (61 Tyr) 62\u201368 hPTH, purified 1\u201384 hPTH and 53\u201384 bPTH showed no ability to displace the tracer indicating that the idiotype of the antibody is directed against the 70\u201384 portion that is usually hidden within the globular structure of the intact molecule. Healthy adults revealed PTH-peptide levels of 153 \u00b1 86 pg\/ml (mean \u00b1 2 <jats:sc>sd<\/jats:sc>); 2 <jats:sc>sd<\/jats:sc> range: 67\u2013239 pg\/ml.<\/jats:p>\n        <jats:p>C-terminal PTH-peptide (70\u201384 hPTH) concentration was normal in patients with primary hyperparathyroidism and chronic renal failure, thus excluding that neither the kidney nor the parathyroid gland is involved in the generation of this C-terminal PTH-peptide sequence. Increased levels of 70\u201384 hPTH immunoreactivity were found in most individuals with severe cholestatic liver disease. Since the liver appears to be responsible for part of the peripheral PTH metabolism, this assay system might be used as an indicator of altered liver function.<\/jats:p>","DOI":"10.1530\/acta.0.1110062","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T21:53:47Z","timestamp":1229378027000},"page":"62-68","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":8,"title":["Selective determination of C-terminal (70\u201384) hPTH: elevated concentrations in cholestatic liver disease"],"prefix":"10.1093","volume":"111","author":[{"given":"E. F.","family":"Rittinghaus","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"J\u00fcppner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Burdelski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. D.","family":"Hesch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/111\/1\/acta_111_1_010.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/111\/1\/acta_111_1_010.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:23:56Z","timestamp":1676391836000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/111\/1\/62\/6763891"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,1]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.1110062","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,1]]}}}