{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T05:27:02Z","timestamp":1676438822041},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1_Suppl","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1987,8]]},"abstract":"<jats:p>Abstract. Primary cell cultures of normal and adenomatous human thyroid tissues were incubated with TSH or ammonium sulphate precipited IGG fractions (1 mg\/ml) of sera from patients with different thyroid diseases (Graves' disease: active n = 7 in remission n = 12; thyroid autonomy n = 39; simple euthyroid goitre n = 15) and were compared to controls (n = 26). [<jats:sup>3<\/jats:sup>H]thymidine incorporation in primary thyrocyte cultures demonstrated a typical bell shape curve after incubation with EGF and TSH with a maximal effect at 10\u2013100 \u03bcIU\/ml. This effect, however, was inconsistent and positive only in 2 of 7 primary cultures. Only TSH positive cultures were used for IgG studies. 16\u201328% of IGG fractions from sera of thyroid patients caused high (more than X + 5 <jats:sc>sd<\/jats:sc> of controls) stimulation of [<jats:sup>3<\/jats:sup>H]thymidine incorporation. Dose response curves of IgG fractions of 19 additional patients (Graves' disease in remission n = 15; thyroid autonomy n = 4) showed an increase in [<jats:sup>3<\/jats:sup>H]thymidine incorporation at 0.1 mg protein\/ml for 10 patients and at low concentrations of 10\u20135 mg\/ml for 5 patients. There was a good correlation (r = 0.72) (<jats:italic>P<\/jats:italic> &lt; 0.0001) between positive findings in TSH-binding inhibition (TBII) and AC-stimulation (TSI) IGG fractions but none between stimulation of [<jats:sup>3<\/jats:sup>H]thymidine incorporation and any other thyroid specific immunoglobulin nor thyroid function nor any other available data. Immunoglobulins stimulating [<jats:sup>3<\/jats:sup>H]thymidine incorporation differ therefore from TBII and TSI. The growth effect of these immunoglobulins, however, has yet to be determined.<\/jats:p>","DOI":"10.1530\/acta.0.114s281","type":"journal-article","created":{"date-parts":[[2009,10,21]],"date-time":"2009-10-21T22:39:20Z","timestamp":1256164760000},"page":"S281-S287","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":2,"title":["Adenylate cyclase stimulation and [3H]thymidine incorporation in human thyroid tissues and thyrocyte cultures: The effect of IgG preparation from patients with different thyroid disorders"],"prefix":"10.1093","volume":"116","author":[{"given":"Peter E.","family":"Goretzki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"West","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rainer","family":"Koob","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Koller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Joseph","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans-Dietrich","family":"R\u00f6her","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/116\/1_Suppl\/acta_114_Suppl_281_051.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/116\/1_Suppl\/acta_114_Suppl_281_051.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:30:35Z","timestamp":1676392235000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/116\/1_Supplement\/S281\/6768644"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,8]]},"references-count":0,"journal-issue":{"issue":"1_Suppl"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.114s281","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,8]]}}}