{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T05:24:39Z","timestamp":1676438679947},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1987,9]]},"abstract":"<jats:p>Abstract. In GH<jats:sub>4<\/jats:sub>C<jats:sub>1<\/jats:sub> cells, the calmodulin antagonist trifluoperazine (TFP) showed a dose-dependent, biphasic effect on the basal release of PRL. An inhibition of PRL release was observed with 15\u201350 \u03bcmol\/l TFP, whereas a concentration of 100 \u03bcmol\/l and above had a stimulatory effect. The increase in basal hormone release evoked by TRH (1 \u03bcmol\/l) and high extracellular concentration of K<jats:sup>+<\/jats:sup> (50 mmol\/l) was eliminated by 30 \u03bcmol\/l TFP. The stimulatory effect of 100 \u03bcmol\/l TFP on basal hormone release was not affected by addition of TRH (1 \u03bcmol\/l) or K<jats:sup>+<\/jats:sup> (50 mmol\/l). The Ca<jats:sup>2+<\/jats:sup> antagonists Co<jats:sup>2+<\/jats:sup> (5 mmol\/l) and verapamil (100 \u03bcmol\/l), and the Ca<jats:sup>2+<\/jats:sup> chelator EgTA (4 mmol\/l) abolished the stimulatory effect of TRH (1 \u03bcmol\/l) and of K<jats:sup>+<\/jats:sup> (50 mmol\/l on PRL release, whereas only Co<jats:sup>2+<\/jats:sup> inhibited the stimulation caused by 100 \u03bcmol\/l TFP. TFP (75 \u03bcmol\/l) caused a transient increase in the concentration of cellular cAMP. Incubation of intact GH<jats:sub>4<\/jats:sub>C<jats:sub>1<\/jats:sub> cells with TFP (75 \u03bcmol\/l), had an inhibitory effect on both the low and the high affinity form of cAMP phosphodiesterase. Basal as well as TRH-stimulated adenyl cyclase activity were inhibited by TFP, and this effect was counteracted by addition of calmodulin.<\/jats:p>","DOI":"10.1530\/acta.0.1160027","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T22:02:37Z","timestamp":1229378557000},"page":"27-35","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":0,"title":["Effects of trifluoperazine on prolactin release and cyclic AMP formation and degradation in GH4C1 pituitary cells"],"prefix":"10.1093","volume":"116","author":[{"given":"Kjersti","family":"Sletholt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Gordeladze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Egil","family":"Haug","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaare M.","family":"Gautvik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/116\/1\/acta_116_1_004.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/116\/1\/acta_116_1_004.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:31:10Z","timestamp":1676392270000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/116\/1\/27\/6764356"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,9]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.1160027","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,9]]}}}