{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T05:23:47Z","timestamp":1676438627299},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"4","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,4]]},"abstract":"<jats:p>Abstract. Brattleboro rats with hereditary diabetes insipidus make it possible to investigate effects of the urea concentration on the in vitro and in vivo production of prostaglandins E<jats:sub>2<\/jats:sub> and F<jats:sub>2\u03b1<\/jats:sub> (PGE<jats:sub>2<\/jats:sub> and PGF<jats:sub>2\u03b1<\/jats:sub>) by the renal papilla independently of any vasopressin effects. The rates of prostaglandin production in vitro are increasing between 100 and 1030 mmol\/l urea and decreasing above 1030 mmol\/l. The ratio PGE<jats:sub>2<\/jats:sub>\/PGF<jats:sub>2\u03b1<\/jats:sub> remains constant at about 4. Normally hydrated and 24 h water-deprived animals in steady state of urine formation were compared in vivo. Urine osmolality increased from 167 \u00b1 6 (N = 5) to 1113 \u00b1 35 (N = 15) mosmol\/kg water and papillary urea concentration from 50 \u00b1 7 to 304 \u00b1 19 mmol\/l after water deprivation. Urinary excretion rates of PGF<jats:sub>2\u03b1<\/jats:sub> increased from 0.83 \u00b1 0.12 to 3.80 \u00b1 0.37 ng\/h. The excretion of PGE<jats:sub>2<\/jats:sub> was unaffected. PGE<jats:sub>2<\/jats:sub> + PGF<jats:sub>2\u03b1<\/jats:sub> excretion rates increased from 1.62 \u00b1 0.25 to 4.61 \u00b1 0.42 ng\/h. These values are in accordance with values predicted from work with Sprague-Dawley rats. Together with previously published data on Sprague-Dawley rats these results indicate that variations of prostaglandin production in the conscious rat in steady state of urine formation can be accounted for by variations of plasma vasopressin and of papillary urea concentration. Variations in the excretion fraction are due to other causes.<\/jats:p>","DOI":"10.1530\/acta.0.1200459","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T22:02:37Z","timestamp":1229378557000},"page":"459-465","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":1,"title":["Effects of urea on the in vitro production of prostaglandins and on urinary excretion in Brattleboro rats"],"prefix":"10.1093","volume":"120","author":[{"given":"Eigil","family":"Bojesen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inge N.","family":"Bojesen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/120\/4\/acta_120_4_010.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/120\/4\/acta_120_4_010.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:34:39Z","timestamp":1676392479000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/120\/4\/459\/6771740"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,4]]},"references-count":0,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.1200459","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,4]]}}}