{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:39:08Z","timestamp":1773301148017,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p>Complete fasting induces a significant decrease of serum T<jats:sub>3<\/jats:sub> and a fall in TSH in rodents and man. To evaluate the effect of starvation on thyroidal T<jats:sub>4<\/jats:sub> 5'monodeiodinating activity, in vitro conversion of T<jats:sub>4<\/jats:sub> to T<jats:sub>3<\/jats:sub> by thyroid and liver homogenate from one to three days fasted mice was compared with homogenates from control mice on animal chow. 5'monodeiodinating activity was significantly lower in thyroid homogenates of fasted mice than in those of chow-fed control [100\u00b15.0 and 92\u00b15.0 pmol T<jats:sub>3<\/jats:sub>\u00b7(mg protein)<jats:sup>\u22121<\/jats:sup>\u00b7h<jats:sup>\u22121<\/jats:sup> at 48 and 72 h fasting, respectively, vs 132\u00b15.0 pmol T<jats:sub>3<\/jats:sub>\u00b7(mg protein)<jats:sup>\u22121<\/jats:sup>\u00b7h<jats:sup>\u22121<\/jats:sup> of fed control, p&lt;0.01]. A similar decrease in thyroidal 5'monodeiodinating activity was seen in the liver. The decrease in thyroidal 5'monodeiodinating activity induced by fasting was not reversed by the supplementation of homogenates with the thiol-protecting agent, dithiothreitol (0.2\u20134.0 mmol\/l). Physiological replacement of T<jats:sub>4<\/jats:sub>, 0.58 nmol\u00b7(100 g)<jats:sup>\u22121<\/jats:sup>\u00b7day<jats:sup>\u22121<\/jats:sup>, did not alter the effect of starvation in either the thyroid or liver. TSH (0.02 IU\/day) injection, on the other hand, stimulated 5'monodeiodinating activity in homogenates of thyroids from 3-days fasted mice which was no different from TSH-treated fed control. It is postulated that starvation-induced decrease in thyroidal T<jats:sub>4<\/jats:sub> to T<jats:sub>3<\/jats:sub> converting activity may play a role, together with decreased hepatic 5'monodeiodinating activity, in fasting-induced low serum T<jats:sub>3<\/jats:sub> in mice.<\/jats:p>","DOI":"10.1530\/acta.0.1220175","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T22:02:37Z","timestamp":1229378557000},"page":"175-180","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":5,"title":["The effect of fasting on thyroidal T4-5'monodeiodinating activity in mice"],"prefix":"10.1093","volume":"122","author":[{"given":"Sing-yung","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/122\/2\/acta_122_2_005.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/122\/2\/acta_122_2_005.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:11:08Z","timestamp":1676391068000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/122\/2\/175\/6771134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,2]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.1220175","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,2]]}}}