{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:13:09Z","timestamp":1761765189106},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,7]]},"abstract":"<jats:title>Abstract.<\/jats:title>\n<jats:p>The binding of [<jats:sup>3<\/jats:sup>H]dihydrotestosterone (17\u03b2-hydroxy-5\u03b1-androstan-3-one) to the androgen receptor was studied in 26 prepubertal boys with isolated hypospadias (no associated anomaly) using fibroblasts cultured from surgical specimens of hypospadiac penile skin and\/or chordal tissue. Maximal binding capacities of androgen receptor for [<jats:sup>3<\/jats:sup>H] dihydrotestosterone in 10 patients with mild degree (2 glandular+8 penile), 6 with severe degree (4 penoscrotal + 2 scrotal), chordal tissues of hypospadias (N=10), and controls (10 endocrinologically normal boys) were, respectively, 11750\u00b12410, 10720\u00b12680, 16100\u00b17570, and 11570\u00b13830 (mean \u00b1 <jats:sc>sd<\/jats:sc>) sites per cell. Dissociation constants in these subjects were 1.09\u00b10.29,0.93\u00b10.22,1.20\u00b10.31, and 1.22\u00b10.41 \u00d7 10<jats:sup>\u221210<\/jats:sup> mol\/l respectively. There were no significant differences as to both maximal binding capacity and dissociation constant of androgen receptor among any groups. Abnormal receptor thermolability was not found in 5 patients with severe degree of hypospadias either. It is, therefore, concluded that prepubertal boys with isolated hypospadias have primarily normal androgen receptor activity.<\/jats:p>","DOI":"10.1530\/acta.0.1230024","type":"journal-article","created":{"date-parts":[[2009,6,11]],"date-time":"2009-06-11T17:28:10Z","timestamp":1244741290000},"page":"24-29","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":10,"title":["Androgen receptor levels in patients with isolated hypospadias"],"prefix":"10.1093","volume":"123","author":[{"given":"Tomoyoshi","family":"Terakawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Shima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidenori","family":"Yabumoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Koyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumihiko","family":"Ikoma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/123\/1\/acta_123_1_005.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/123\/1\/acta_123_1_005.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:11:50Z","timestamp":1676391110000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/123\/1\/24\/6768941"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,7]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.1230024","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,7]]}}}