{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T13:05:27Z","timestamp":1776344727195,"version":"3.51.2"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,8]]},"abstract":"<jats:title>Abstract.<\/jats:title>\n<jats:p>The sera from two patients with Graves' disease gave abnormally high serum free T<jats:sub>4<\/jats:sub> values as compared with the total T<jats:sub>4<\/jats:sub> and other hormone values, suggesting the presence of autoantibodies to labelled T<jats:sub>4<\/jats:sub> analogue used in the Amersham free T<jats:sub>4<\/jats:sub> assay kit. The autoantibodies appeared to develop after the initiation of methimazole therapy and disappeared again after the cessation of methimazole. This binding activity to labelled T<jats:sub>4<\/jats:sub> analogue was demonstrated to be in the immunoglobulin G with a <jats:italic>k<\/jats:italic> light chain isotype in both sera, and was displaced by unlabelled T<jats:sub>4<\/jats:sub> in a dose-dependent manner. The binding of immunoglobulin G purified from these sera to labelled T<jats:sub>4<\/jats:sub> or T<jats:sub>4<\/jats:sub> analogue was found to be almost identical to that of the corresponding serum binding. Since the specific radioactivity of labelled T<jats:sub>4<\/jats:sub> analogue used in the Amersham free T<jats:sub>4<\/jats:sub> assay kit is about 10 times higher than that of the labelled T<jats:sub>4<\/jats:sub> in the Amersham total T<jats:sub>4<\/jats:sub> assay kit, serum free T<jats:sub>4<\/jats:sub> determinations are much more vulnerable to thyroid hormone autoantibodies. Thus, in the presence of autoantibodies, a large discrepancy develops between free T<jats:sub>4<\/jats:sub> and total T<jats:sub>4<\/jats:sub> values.<\/jats:p>","DOI":"10.1530\/acta.0.1230123","type":"journal-article","created":{"date-parts":[[2009,6,11]],"date-time":"2009-06-11T17:28:10Z","timestamp":1244741290000},"page":"123-128","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":5,"title":["The mechanism for the discrepancy between serum total and free thyroxine values induced by autoantibodies: Report on two patients with Graves' disease"],"prefix":"10.1093","volume":"123","author":[{"given":"Makoto I","family":"Iitaka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuhiko","family":"Fukasawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihito","family":"Hara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morifumi","family":"Yanagisawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazumasa","family":"Hase","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiro","family":"Miura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiki","family":"Sakatsume","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Ishii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["Acta Endocrinologica"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/123\/2\/acta_123_2_001.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/123\/2\/acta_123_2_001.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:11:57Z","timestamp":1676391117000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/123\/2\/123\/6768921"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,8]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1530\/acta.0.1230123","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,8]]}}}