{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:17:14Z","timestamp":1784557034603,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"11","license":[{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["ec.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,11]]},"abstract":"\n Objective<\/jats:title>\n Vitamin D deficiency is associated with increased risks of arterial and venous cardiovascular events. Hypothetically, supplementation with vitamin D may lead to a less prothrombotic phenotype, as measured by global coagulation assays and fibrin clot structure.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n In this prospective cohort study, we enrolled adult outpatients attending the Primary Care Division of the Geneva University Hospitals with a severe vitamin D deficiency (25-hydroxyvitamin-D3 (25-OHD) <25 nmol\/L), excluding obese patients or with a recent acute medical event. We evaluated changes in coagulation times, thrombin generation assay, clot formation and clot lysis time, 25-OHD and parathormone before and 1\u20133 months after cholecalciferol oral supplementation with one-time 300,000 IU then 800 IU daily. Paired\n t<\/jats:italic>\n -tests with a two-sided alpha of 0.05 compared absolute mean differences.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The 48 participants had a mean age of 43.8\u2009\u00b1\u200913.8 years. After supplementation, 25-OHD levels increased from 17.9\u2009\u00b1\u20094.6 nmol\/L to 62.5\u2009\u00b1\u200920.7 nmol\/L 6.4\u2009\u00b1\u20093.0 weeks after inclusion. Endogenous thrombin potential and thrombin generation peak values both decreased significantly (\u221295.4 nM\u2009\u00d7\u2009min (95%CI \u2212127.9 to \u221262.8),\n P<\/jats:italic>\n \u2009<\u20090.001; \u221215.1 nM (\u221223.3 to \u22126.8),\n P<\/jats:italic>\n \u2009<\u20090.001). The maximum absorbance by turbidimetry decreased significantly (\n P<\/jats:italic>\n \u2009=\u20090.001) after supplementation. There was no change in clot lysis time, coagulation times or plasminogen activator inhibitor-1 and homocysteine levels.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n In severe vitamin D deficiency, a high-dose cholecalciferol supplementation was associated with a reduction in thrombin generation and an average decreased number of fibrin protofibrils per fibers and fibrin fiber size measured by turbidimetry. This suggests that severe vitamin D deficiency may be associated with a potentially reversible prothrombotic profile.<\/jats:p>\n <\/jats:sec>","DOI":"10.1530\/ec-19-0429","type":"journal-article","created":{"date-parts":[[2019,10,15]],"date-time":"2019-10-15T12:37:22Z","timestamp":1571143042000},"page":"1447-1454","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-10","source":"Crossref","is-referenced-by-count":22,"title":["Thrombin generation and fibrin clot structure after vitamin D supplementation"],"prefix":"10.1530","volume":"8","author":[{"given":"Marc","family":"Blondon","sequence":"first","affiliation":[{"name":"1Division of Angiology and Hemostasis, Geneva University Hospitals and Faculty of Medicine, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Biver","sequence":"additional","affiliation":[{"name":"2Division of Bone Diseases, Geneva University Hospitals and Faculty of Medicine, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia","family":"Braillard","sequence":"additional","affiliation":[{"name":"3Division of Primary Care Medicine, Geneva University Hospitals and Faculty of Medicine, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Righini","sequence":"additional","affiliation":[{"name":"1Division of Angiology and Hemostasis, Geneva University Hospitals and Faculty of Medicine, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Fontana","sequence":"additional","affiliation":[{"name":"1Division of Angiology and Hemostasis, Geneva University Hospitals and Faculty of Medicine, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Casini","sequence":"additional","affiliation":[{"name":"1Division of Angiology and Hemostasis, Geneva University Hospitals and Faculty of Medicine, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Endocrine Connections"],"original-title":[],"link":[{"URL":"https:\/\/ec.bioscientifica.com\/view\/journals\/ec\/8\/11\/EC-19-0429.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ec.bioscientifica.com\/downloadpdf\/journals\/ec\/8\/11\/EC-19-0429.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:43:45Z","timestamp":1784555025000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/ec\/article\/8\/11\/1447-1454\/604"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11]]},"references-count":0,"journal-issue":{"issue":"11"},"URL":"https:\/\/doi.org\/10.1530\/ec-19-0429","relation":{},"ISSN":["2049-3614"],"issn-type":[{"value":"2049-3614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11]]}}}