{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:17:09Z","timestamp":1784557029258,"version":"3.55.0"},"reference-count":29,"publisher":"Bioscientifica","issue":"4","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["ec.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,4,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The objective of this study is to investigate the role of serum irisin level in diagnosis of central precocious puberty (CPP) in girls and its major determinants.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study was conducted in 67 girls with CPP, 19 girls with premature thelarche (PT) and 59 normal controls. The major determinants of irisin were assessed by multivariate linear regression (MLR) analysis. Propensity score matching (PSM) analysis was performed to minimize the bias that can result from BMI. A receiver operating characteristic curve was used to obtain the optimal threshold value of irisin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The girls with CPP and PT had higher irisin levels than controls (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009 &lt; 0.05). The optimal cutoff value of irisin levels for predicting CPP was 91.88 ng\/mL, with a sensitivity of 70.1% and a specificity of 72.9%. MLR analysis showed that BMI was a predictor of irisin (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009 &lt; 0.05). Serum irisin levels remained higher in the CPP girls than the controls with adjustment for BMI (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009 &lt; 0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Increased serum irisin levels with CPP suggest that irisin is involved in puberty. However, due to low sensitivity and specificity, irisin level can only be used as an auxiliary indicator rather than a single diagnostic indicator of CPP.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/ec-22-0028","type":"journal-article","created":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T11:30:20Z","timestamp":1646739020000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-10","source":"Crossref","is-referenced-by-count":5,"title":["Serum irisin levels increase in girls with central precocious puberty not dependent on BMI: a pilot study"],"prefix":"10.1530","volume":"11","author":[{"given":"Yanfei","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binrong","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingzi","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5899-7040","authenticated-orcid":true,"given":"Dan","family":"Lan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"bib1","series-title":"Zhong hua Er Ke Za Zhi","first-page":"412","article-title":"Consensus statement for the diagnosis and treatment of central precocious puberty (2015)","volume":"53","year":"2015","unstructured":"Subspecialty Group of Endocrinologic, Hereditary and Metabolic Diseases, the Society of Pediatrics, Chinese Medical Association & Editorial Board, Chinese Journal of Pediatrics. 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(https:\/\/doi.org\/10.1016\/j.jpeds.2018.12.022)3085777710.1016\/j.jpeds.2018.12.022"},{"key":"bib3","series-title":"JAMA Network Open","article-title":"Trends in the incidence of central precocious puberty and normal variant puberty among children in Denmark, 1998 to 2017","volume":"3","author":"Br\u00e4uner","year":"2020","unstructured":"Br\u00e4uner EVBusch ASEckert-Lind CKoch THickey MJuul A. Trends in the incidence of central precocious puberty and normal variant puberty among children in Denmark, 1998 to 2017. JAMA Network Open20203 e2015665. (https:\/\/doi.org\/10.1001\/jamanetworkopen.2020.15665)33044548"},{"key":"bib4","series-title":"JAMA Pediatrics","article-title":"Worldwide secular trends in age at pubertal onset assessed by breast development among girls: a systematic review and meta-analysis","volume":"174","author":"Eckert-Lind","year":"2020","unstructured":"Eckert-Lind CBusch ASPetersen JHBiro FMButler GBr\u00e4uner EVJuul A. Worldwide secular trends in age at pubertal onset assessed by breast development among girls: a systematic review and meta-analysis. JAMA Pediatrics2020174 e195881. (https:\/\/doi.org\/10.1001\/jamapediatrics.2019.5881)32040143"},{"key":"bib5","series-title":"Journal of Pediatrics","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1016\/j.jpeds.2020.05.013","article-title":"Changes in children\u2019s healthcare visits during coronavirus disease-2019 pandemic in Hangzhou, China","volume":"224","author":"Li","year":"2020","unstructured":"Li HYu GDuan HFu JShu Q. Changes in children\u2019s healthcare visits during coronavirus disease-2019 pandemic in Hangzhou, China. Journal of Pediatrics2020224146\u2013149. (https:\/\/doi.org\/10.1016\/j.jpeds.2020.05.013)3241608710.1016\/j.jpeds.2020.05.013"},{"key":"bib6","series-title":"Italian Journal of Pediatrics","article-title":"Increased incidence of precocious and accelerated puberty in females during and after the Italian lockdown for the coronavirus 2019 (COVID-19) pandemic","volume":"46","author":"Stagi","year":"2020","unstructured":"Stagi SDe Masi SBencini ELosi SPaci SParpagnoli MRicci FCiofi DAzzari C. Increased incidence of precocious and accelerated puberty in females during and after the Italian lockdown for the coronavirus 2019 (COVID-19) pandemic. Italian Journal of Pediatrics202046 165. (https:\/\/doi.org\/10.1186\/s13052-020-00931-3)33148304"},{"key":"bib7","series-title":"Human Reproduction Update","doi-asserted-by":"publisher","first-page":"737","DOI":"10.1093\/humupd\/dmx025","article-title":"Disentangling puberty: novel neuroendocrine pathways and mechanisms for the control of mammalian puberty","volume":"23","author":"Avenda\u00f1o","year":"2017","unstructured":"Avenda\u00f1o MSVazquez MJTena-Sempere M. Disentangling puberty: novel neuroendocrine pathways and mechanisms for the control of mammalian puberty. Human Reproduction Update201723737\u2013763. 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