{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:17:27Z","timestamp":1784557047025,"version":"3.55.0"},"reference-count":61,"publisher":"Bioscientifica","issue":"8","license":[{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["ec.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,8,1]]},"abstract":"<jats:p>\n                    Glucocorticoids have short- and long-term effects on adrenal gland function and development. RNA sequencing (RNA-seq) was performed to identify early transcriptomic responses to the synthetic glucocorticoid, dexamethasone (Dex),\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . In total, 1711 genes were differentially expressed in the adrenal glands of the 1-h Dex-treated mice. Among them, only 113 were also considered differentially expressed genes (DEGs) in murine adrenocortical Y-1 cells treated with Dex for 1 h. Gene ontology analysis showed that the upregulated DEGs in the adrenal gland of the 1-h Dex-treated mice were highly associated with the development of neuronal cells, suggesting the adrenal medulla had a rapid response to Dex. Interestingly, only 4.3% of Dex-responsive genes in the Y-1 cell line under Dex treatment for 1 h were differentially expressed under Dex treatment for 24 h. The heatmaps revealed that most early responsive DEGs in Y-1 cells during 1 h of treatment exhibited a transient response. The expression of these genes under treatment for 24 h returned to basal levels similar to that during control treatment. In summary, this research compared the rapid transcriptomic effects of Dex stimulation\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Notably, adrenocortical Y-1 cells had a transient early response to Dex treatment. Furthermore, the DEGs had a minimal overlap in the 1-h Dex-treated group\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1530\/ec-22-0064","type":"journal-article","created":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T18:07:41Z","timestamp":1656007661000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-10","source":"Crossref","is-referenced-by-count":6,"title":["Early transcriptomic response of mouse adrenal gland and Y-1 cells to dexamethasone"],"prefix":"10.1530","volume":"11","author":[{"given":"Huifei Sophia","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey G","family":"Daniel","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia M","family":"Salamat","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laci","family":"Mackay","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad D","family":"Foradori","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J","family":"Kemppainen","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satyanarayana R","family":"Pondugula","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Xiong","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0376-4682","authenticated-orcid":true,"given":"Chen-Che Jeff","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"bib1","series-title":"Nature Clinical Practice: Rheumatology","first-page":"525","article-title":"Genomic and nongenomic effects of glucocorticoids","volume":"4","author":"Stahn","year":"2008","unstructured":"Stahn CButtgereit F. 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