{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T09:03:01Z","timestamp":1792141381020,"version":"4.4.0"},"reference-count":49,"publisher":"Bioscientifica","issue":"1","license":[{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"vor","delay-in-days":449,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,1,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Androgen insensitivity syndrome (AIS) due to androgen receptor (AR) mutations creates a spectrum of clinical presentations based on residual AR function with the mildest impairment creating mild AIS (MAIS) whose undefined molecular mechanism and subtle clinical features leave it less understood and underdiagnosed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>In silico modeling and in vitro androgen bioassay of the mutated AR are used to identify its structural and physiological mechanism. Clinical features and responses to high-dose testosterone treatment of three cases of MAIS across a six-generation family pedigree are described.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Structural and dynamic in silico molecular modeling and in vitro yeast-based androgen bioassays of the mutant AR are employed. Three cases of MAIS with consistent (gynecomastia and micropenis) and variable (infertility) clinical features across generations are reported, and the effects of high-dose testosterone treatment are studied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The missense AR exon 8 mutation (nucleotide aga \u2192 gga, p.R872G arginine to glycine), known to cause an increased ligand dissociation rate in mutant AR in binding assays, was analyzed. Modeling shows that the mutation weakens the closure energy of the \u2018lid\u2019 of the ligand-binding pocket, allowing easier ligand dissociation from the binding site but with unimpaired in vitro androgen bioactivity. High-dose testosterone treatment for 3 years in one young man caused increased virilization and height growth but was ineffective for treating micropenis. Genetic counseling allowed effective prediction of MAIS risks in progeny for carrier and noncarrier sisters.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The differential diagnosis and clinical management of MAIS is reviewed. The novel molecular mechanism of an AR ligand-binding domain mutation in MAIS may be present in other cases of MAIS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/ec-24-0567","type":"journal-article","created":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T06:20:16Z","timestamp":1731910816000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-10","source":"Crossref","is-referenced-by-count":2,"title":["Molecular mechanism of androgen receptor mutation in multigenerational mild androgen insensitivity syndrome"],"prefix":"10.1530","volume":"14","author":[{"given":"Ravind","family":"Pandher","sequence":"first","affiliation":[{"name":"Andrology Department 1 , ,","place":["Concord, Australia"]},{"name":"ANZAC Research Institute, University of Sydney, Concord Hospital 2 , ,","place":["Concord, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruby","family":"Chang","sequence":"additional","affiliation":[{"name":"Andrology Department 1 , ,","place":["Concord, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqun","family":"Chang","sequence":"additional","affiliation":[{"name":"Sydney Pharmacy School, Faculty of Medicine and Health, The University of Sydney 3 , ,","place":["Camperdown, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2635-2990","authenticated-orcid":true,"given":"David E","family":"Hibbs","sequence":"additional","affiliation":[{"name":"Sydney Pharmacy School, Faculty of Medicine and Health, The University of Sydney 3 , ,","place":["Camperdown, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7750-4413","authenticated-orcid":true,"given":"Jonathan J","family":"Du","sequence":"additional","affiliation":[{"name":"Sydney Pharmacy School, Faculty of Medicine and Health, The University of Sydney 3 , ,","place":["Camperdown, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6244-3929","authenticated-orcid":true,"given":"Kristine","family":"McGrath","sequence":"additional","affiliation":[{"name":"University of Technology 4 , , ,","place":["Sydney, New South Wales, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5449-3478","authenticated-orcid":true,"given":"Alison","family":"Heather","sequence":"additional","affiliation":[{"name":"University of Otago 5 , ,","place":["Dunedin, New Zealand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veena","family":"Jayadev","sequence":"additional","affiliation":[{"name":"Andrology Department 1 , ,","place":["Concord, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4200-7476","authenticated-orcid":true,"given":"David J","family":"Handelsman","sequence":"additional","affiliation":[{"name":"Andrology Department 1 , ,","place":["Concord, Australia"]},{"name":"ANZAC Research Institute, University of Sydney, Concord Hospital 2 , ,","place":["Concord, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","published-online":{"date-parts":[[2024,11,18]]},"reference":[{"key":"2026070710554618200_bib1","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1124\/pr.58.4.9","article-title":"International Union of Pharmacology. 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