{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T16:21:10Z","timestamp":1783441270046,"version":"3.54.6"},"reference-count":33,"publisher":"Bioscientifica","issue":"10","license":[{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"vor","delay-in-days":132,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,10,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with the function of granulosa cells. This study was conducted to explore the regulatory mechanism of Kruppel-like factor 7 (KLF7) in the proliferation and apoptosis of granulosa cells and provide a rationale for the treatment of PCOS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Immortalized human ovarian granulosa cells HGL5 were cultured in vitro and treated with dihydrotestosterone (DHT) to establish the PCOS cell model. HGL5 cells were transfected with KLF7 and phosphodiesterase 4 (PDE4) overexpression vectors, and the KLF7\/PDE4 expression levels in HGL5 cells were determined by qRT-PCR and Western blot. Cell viability, apoptosis, and proliferation were assessed by CCK-8, TUNEL staining, and EdU assays. The interaction between PDE4 promoter and KLF7 was testified by chromatin immunoprecipitation and dual-luciferase assay. Collaborative experiments were conducted to validate the mechanism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>KLF7 was downregulated in DHT-treated HGL5 cells. KLF7 overexpression facilitated the proliferation and inhibited the apoptosis of DHT-treated HGL5 cells. KLF7 bound to the PDE4 promoter and then repressed its transcription and protein levels. PDE4 overexpression neutralized the effects of KLF7 overexpression on DHT-treated HGL5 cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>KLF7 inhibits apoptosis and promotes proliferation of PCOS cells by transcriptionally inhibiting PDE4 expression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/ec-25-0608","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T17:36:13Z","timestamp":1760117773000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-10","source":"Crossref","is-referenced-by-count":0,"title":["Mechanism of KLF7 in regulating the proliferation and apoptosis of granulosa cells in polycystic ovarian syndrome"],"prefix":"10.1530","volume":"14","author":[{"given":"Honghong","family":"Wang","sequence":"first","affiliation":[{"name":"Center for Reproductive Medicine, Children\u2019s Hospital of Shanxi and Women Health Center of Shanxi 1 , ,","place":["Taiyuan, China"]},{"name":"2 Shanxi Center of Technology Innovation for Fertility Optimination, Taiyuan, Shanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhua","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Children\u2019s Hospital of Shanxi and Women Health Center of Shanxi 1 , ,","place":["Taiyuan, China"]},{"name":"2 Shanxi Center of Technology Innovation for Fertility Optimination, Taiyuan, Shanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiumei","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Children\u2019s Hospital of Shanxi and Women Health Center of Shanxi 1 , ,","place":["Taiyuan, China"]},{"name":"2 Shanxi Center of Technology Innovation for Fertility Optimination, Taiyuan, Shanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suming","family":"Xu","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Children\u2019s Hospital of Shanxi and Women Health Center of Shanxi 1 , ,","place":["Taiyuan, China"]},{"name":"2 Shanxi Center of Technology Innovation for Fertility Optimination, Taiyuan, Shanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6649-9050","authenticated-orcid":true,"given":"Xueqing","family":"Wu","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Children\u2019s Hospital of Shanxi and Women Health Center of Shanxi 1 , ,","place":["Taiyuan, China"]},{"name":"2 Shanxi Center of Technology Innovation for Fertility Optimination, Taiyuan, Shanxi, 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