{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,2,18]],"date-time":"2023-02-18T21:06:26Z","timestamp":1676754386603},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"5","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Endothelial nitric oxide synthase (eNOS) intron 4a\/b polymorphism is associated with plasma NO concentrations and coronary artery disease\/hypertension in various populations. GH deficiency in adulthood predisposes to reduced NO concentrations and premature atherosclerosis. Our aim was to determine whether intron 4a\/b polymorphism of eNOS gene influences endothelial function and early atherosclerotic changes in GH-deficient hypopituitary patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Thirty-three hypopituitary GH-deficient patients on conventional replacement therapy other than GH and 43 age-, sex-, and body mass index (BMI)-matched controls were studied in this cross-sectional case\u2013control study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Early atherosclerotic changes were determined by flow-mediated dilation (FMD) of brachial artery and carotid artery intima-media thickness (IMT). eNOS4a\/b polymorphism was detected by PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hypopituitary patients had significantly higher total\/low-density lipoprotein cholesterol and fat mass and lower IGF-I concentrations compared with controls. IMT was significantly higher in patients (0.777\u00b10.23 vs 0.639\u00b10.17\u200amm, <jats:italic>P<\/jats:italic>&lt;0.01). No significant difference was observed with respect to FMD measurements. eNOS4a\/b genotype frequencies were similar between patients and controls. Patients carrying \u2018a\u2019 allele (a\/a and a\/b) had significantly higher IMT compared with controls carrying \u2018a\u2019 allele and bb genotype (<jats:italic>P<\/jats:italic>&lt;0.05). However, logistic regression analysis revealed that presence of hypopituitarism, age\u226545 years, and BMI\u226527.9\u200akg\/m<jats:sup>2<\/jats:sup> were significant independent predictors of IMT\u22650.65\u200amm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>No compelling data are evident to suggest that eNOS4a\/b polymorphism modifies the atherosclerotic process in GH-deficient situations. A large case\u2013control study is needed to confirm our findings.<\/jats:p><\/jats:sec>","DOI":"10.1530\/eje-07-0704","type":"journal-article","created":{"date-parts":[[2008,4,22]],"date-time":"2008-04-22T00:59:47Z","timestamp":1208825987000},"page":"615-622","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":3,"title":["Endothelial nitric oxide synthase intron 4a\/b polymorphism and early atherosclerotic changes in hypopituitary GH-deficient adult patients"],"prefix":"10.1093","volume":"158","author":[{"given":"Fatma","family":"Sen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustafa","family":"Demirturk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neslihan","family":"Abaci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ebru","family":"Golcuk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huseyin","family":"Oflaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Elitok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faruk","family":"Kutluturk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Halim","family":"Issever","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nihan Erginel","family":"Unaltuna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nese Colak","family":"Ozbey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["European Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/158\/5\/615.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/158\/5\/615.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:21:16Z","timestamp":1676391676000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/158\/5\/615\/6675885"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5]]},"references-count":0,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.1530\/eje-07-0704","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,5]]}}}