{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T07:53:57Z","timestamp":1772697237551,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"5","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Mice with osteoblast-specific deletion of parathyroid hormone-related protein (PTHrP) exhibit impaired recruitment and increased apoptosis of osteogenic cells resulting in decreased bone formation and premature osteoporosis. The PTHrP levels within the bone microenvironment are therefore critical in influencing bone mass acquisition. Whether this is applicable in humans has not been established. Here, we studied the association of a variable number of tandem repeats (VNTR) polymorphism in <jats:italic>PTHrP<\/jats:italic> with peak bone mass.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Enrolled in the study were 234 young Finnish males, with median age of 19.6 years (range 18.3\u201320.6 years). Lifestyle factors, serum bone markers, osteodensitometric measurements (lumbar spine and hip) and calcaneal quantitative ultrasound readings were obtained. The <jats:italic>PTHrP<\/jats:italic> VNTR length was determined by the PCR amplification of genomic DNA extracted from peripheral blood and correlated to bone parameters by the multiple regression models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The presence of at least one 252\u200abp allele was associated with increased lumbar spine bone mineral density (BMD; <jats:italic>P<\/jats:italic>&lt;0.0034), broadband ultrasound attenuation (BUA; <jats:italic>P<\/jats:italic>&lt;0.0012) and speed-of-sound (SOS; <jats:italic>P<\/jats:italic>&lt;0.0023) measurements. The correlation with increased lumbar spine BMD (<jats:italic>P<\/jats:italic>=0.0008), BUA (<jats:italic>P<\/jats:italic>=0.005) and SOS (<jats:italic>P<\/jats:italic>=0.001) was further strengthened by the pairing of the 252\u200abp allele with a 460\u200abp allele in comparison with those without any 252\u200abp allele. Electrophoretic mobility shift assays were used to illustrate the potential transcriptional functionality of the VNTR sequence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results indicate that the <jats:italic>PTHrP<\/jats:italic> VNTR sequence likely modulates local <jats:italic>PTHrP<\/jats:italic> expression within the skeletal microenvironment and could serve as a diagnostic predictor of peak bone mass acquisition.<\/jats:p><\/jats:sec>","DOI":"10.1530\/eje-07-0886","type":"journal-article","created":{"date-parts":[[2008,4,22]],"date-time":"2008-04-22T00:59:47Z","timestamp":1208825987000},"page":"755-764","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":10,"title":["Variable number of tandem repeats polymorphism in parathyroid hormone-related protein as predictor of peak bone mass in young healthy Finnish males"],"prefix":"10.1093","volume":"158","author":[{"given":"Ajay","family":"Gupta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ville-Valtteri","family":"V\u00e4lim\u00e4ki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matti","family":"J V\u00e4lim\u00e4ki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliisa","family":"L\u00f6yttyniemi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilyn","family":"Richard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prasanna","family":"L Bukka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Goltzman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"C Karaplis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["European Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/158\/5\/755.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/158\/5\/755.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:21:33Z","timestamp":1676391693000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/158\/5\/755\/6676046"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5]]},"references-count":0,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.1530\/eje-07-0886","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,5]]}}}