{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:16:00Z","timestamp":1762917360375},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.bioscientifica.com\/journals\/reuselicenceeje\/"}],"content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Lymphocytic hypophysitis is an organ-specific autoimmune disease of the pituitary gland. A specific and sensitive serological test currently does not exist to aid in the diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To identify target autoantigens in lymphocytic hypophysitis and develop a diagnostic assay for these proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design\/methods<\/jats:title><jats:p>A pituitary cDNA expression library was immunoscreened using sera from four patients with lymphocytic hypophysitis. Relevant cDNA clones from screening, along with previously identified autoantigens pituitary gland-specific factor 1a and 2 (PGSF1a and PGSF2) and neuron-specific enolase (NSE) were tested in an<jats:italic>in vitro<\/jats:italic>transcription and translation immunoprecipitation assay. The corticotroph-specific transcription factor, TPIT, was investigated separately as a candidate autoantigen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significantly positive autoantibody reactivity against TPIT was found in 9\/86 hypophysitis patients vs 1\/90 controls (<jats:italic>P<\/jats:italic>=0.018). The reactivity against TPIT was not specific for lymphocytic hypophysitis with autoantibodies detectable in the sera from patients with other autoimmune endocrine diseases. Autoantibodies were also detected against chromodomain-helicase-DNA binding protein 8, presynaptic cytomatrix protein (piccolo), Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup>-dependent secretion activator, PGSF2 and NSE in serum samples from patients with lymphocytic hypophysitis, but at a frequency that did not differ from healthy controls. Importantly, 8\/86 patients with lymphocytic hypophysitis had autoantibodies against any two autoantigens in comparison with 0\/90 controls (<jats:italic>P<\/jats:italic>=0.0093).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>TPIT, a corticotroph-specific transcription factor, was identified as a target autoantigen in 10.5% of patients with lymphocytic hypophysitis. Further autoantigens related to vesicle processing were also identified as potential autoantigens with different immunoreactivity patterns in patients and controls.<\/jats:p><\/jats:sec>","DOI":"10.1530\/eje-11-1015","type":"journal-article","created":{"date-parts":[[2011,12,23]],"date-time":"2011-12-23T05:35:12Z","timestamp":1324618512000},"page":"391-398","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":49,"title":["Identification of TPIT and other novel autoantigens in lymphocytic hypophysitis; immunoscreening of a pituitary cDNA library and development of immunoprecipitation assays"],"prefix":"10.1093","volume":"166","author":[{"given":"Casey Jo Anne","family":"Smith","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Bensing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Burns","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phillip J","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna A","family":"Kasperlik-Zaluska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodney J","family":"Scott","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olle","family":"K\u00e4mpe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia A","family":"Crock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","reference":[{"issue":"5","key":"1_39931173","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1210\/er.2004-0011","volume":"26","year":"2005","journal-title":"Endocrine Reviews","ISSN":"http:\/\/id.crossref.org\/issn\/0163-769X","issn-type":"print"},{"issue":"8","key":"2_15960077","doi-asserted-by":"publisher","first-page":"2302","DOI":"10.1210\/jc.80.8.2302","volume":"80","author":"Thodou","year":"1995","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"http:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"2","key":"3_18637501","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1111\/j.1365-2362.2005.01459.x","volume":"35","author":"Bensing","year":"2005","journal-title":"European journal of clinical investigation","ISSN":"http:\/\/id.crossref.org\/issn\/0014-2972","issn-type":"print"},{"issue":"2","key":"4_5888278","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1210\/jc.83.2.609","volume":"83","author":"Crock","year":"1998","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"http:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"2","key":"5_13875385","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1059\/0003-4819-105-2-200","volume":"105","author":"JENSEN","year":"1986","journal-title":"Annals of Internal Medicine","ISSN":"http:\/\/id.crossref.org\/issn\/0003-4819","issn-type":"print"},{"issue":"5","key":"6_22911362","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1507\/endocrj.K06-055","volume":"53","author":"Hashimoto","year":"2006","journal-title":"Endocrine journal","ISSN":"http:\/\/id.crossref.org\/issn\/0918-8959","issn-type":"print"},{"issue":"4","key":"7_16521841","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1507\/endocrj.43.433","volume":"43","author":"Ishihara","year":"1996","journal-title":"Endocrine journal","ISSN":"http:\/\/id.crossref.org\/issn\/0918-8959","issn-type":"print"},{"issue":"3","key":"8_15883602","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/j.1365-2265.1995.tb01881.x","volume":"42","author":"Powrie","year":"1995","journal-title":"Clinical endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0300-0664","issn-type":"print"},{"issue":"6","key":"9_29271900","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/j.autrev.2007.01.006","volume":"6","author":"Bizzaro","year":"2007","journal-title":"Autoimmunity reviews","ISSN":"http:\/\/id.crossref.org\/issn\/1568-9972","issn-type":"print"},{"issue":"9420","key":"10_18177174","doi-asserted-by":"publisher","first-page":"1544","DOI":"10.1016\/S0140-6736(04)16154-0","volume":"363","author":"Scofield","year":"2004","journal-title":"Lancet","ISSN":"http:\/\/id.crossref.org\/issn\/0140-6736","issn-type":"print"},{"issue":"10","key":"11_9314574","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1055\/s-2007-1004967","volume":"22","author":"Gl\u00a0\u00a0ck","year":"1990","journal-title":"Hormone and metabolic research. Hormon- und Stoffwechselforschung. Hormones et m&#x00A0;&#x00A0;tabolisme","ISSN":"http:\/\/id.crossref.org\/issn\/0018-5043","issn-type":"print"},{"key":"12_41979556","doi-asserted-by":"crossref","first-page":"299","DOI":"10.3109\/08916939309079232","volume":"14","year":"1993","ISSN":"http:\/\/id.crossref.org\/issn\/1607-842X","issn-type":"print"},{"issue":"5556","key":"13_4006652","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1038\/261142a0","volume":"261","author":"Pouplard","year":"1976","journal-title":"Nature; Physical Science (London)","ISSN":"http:\/\/id.crossref.org\/issn\/1476-4687","issn-type":"print"},{"issue":"8330","key":"14_8910211","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/S0140-6736(83)91328-4","volume":"1","author":"Scherbaum","year":"1983","journal-title":"Lancet","ISSN":"http:\/\/id.crossref.org\/issn\/0140-6736","issn-type":"print"},{"issue":"8547","key":"15_14156618","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1016\/S0140-6736(87)90592-7","volume":"1","author":"Scherbaum","year":"1987","journal-title":"Lancet","ISSN":"http:\/\/id.crossref.org\/issn\/0140-6736","issn-type":"print"},{"issue":"1","key":"16_10259298","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/0022-1759(93)90404-U","volume":"162","author":"Crock","year":"1993","journal-title":"Journal of immunological methods","ISSN":"http:\/\/id.crossref.org\/issn\/0022-1759","issn-type":"print"},{"issue":"6","key":"17_17353188","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1530\/eje.0.1470767","volume":"147","author":"Tanaka","year":"2002","journal-title":"European Journal of Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0804-4643","issn-type":"print"},{"issue":"6","key":"18_17985101","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1507\/endocrj.50.697","volume":"50","author":"Tanaka","year":"2003","journal-title":"Endocrine journal","ISSN":"http:\/\/id.crossref.org\/issn\/0918-8959","issn-type":"print"},{"issue":"2","key":"19_16908737","doi-asserted-by":"publisher","first-page":"752","DOI":"10.1210\/jc.87.2.752","volume":"87","author":"O'Dwyer","year":"2002","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"http:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"1-2","key":"20_16993290","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1076\/apab.110.1.94.897","volume":"110","author":"O'Dwyer","year":"2002","journal-title":"Archives of physiology and biochemistry","ISSN":"http:\/\/id.crossref.org\/issn\/1381-3455","issn-type":"print"},{"issue":"6","key":"21_16882111","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1159\/000050015","volume":"55","author":"Takao","year":"2001","journal-title":"Hormone research","ISSN":"http:\/\/id.crossref.org\/issn\/0301-0163","issn-type":"print"},{"issue":"4","key":"22_10544865","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1507\/endocrj.47.407","volume":"47","author":"Kikuchi","year":"2000","journal-title":"Endocrine journal","ISSN":"http:\/\/id.crossref.org\/issn\/0918-8959","issn-type":"print"},{"issue":"2","key":"23_28007235","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/BF03347415","volume":"30","author":"Bensing","year":"2007","journal-title":"Journal of endocrinological investigation","ISSN":"http:\/\/id.crossref.org\/issn\/0391-4097","issn-type":"print"},{"issue":"2","key":"24_31761393","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1111\/j.1365-2265.2008.03180.x","volume":"69","author":"Lupi","year":"2008","journal-title":"Clinical endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0300-0664","issn-type":"print"},{"issue":"6","key":"25_11099978","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1016\/S0092-8674(01)00282-3","volume":"104","author":"Lamolet","year":"2001","journal-title":"Cell","ISSN":"http:\/\/id.crossref.org\/issn\/0092-8674","issn-type":"print"},{"issue":"6","key":"26_17550587","doi-asserted-by":"publisher","first-page":"738","DOI":"10.1101\/gad.1065703","volume":"17","year":"2003","journal-title":"Genes &amp; Development","ISSN":"http:\/\/id.crossref.org\/issn\/0890-9369","issn-type":"print"},{"key":"27_34475611","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1080\/0891693031000095871","volume":"36","year":"2003","ISSN":"http:\/\/id.crossref.org\/issn\/1607-842X","issn-type":"print"},{"issue":"3","key":"28_23508185","doi-asserted-by":"publisher","first-page":"949","DOI":"10.1073\/pnas.0610070104","volume":"104","year":"2007","journal-title":"PNAS","ISSN":"http:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"19","key":"29_16017232","doi-asserted-by":"publisher","first-page":"8626","DOI":"10.1073\/pnas.92.19.8626","volume":"92","author":"Rorsman","year":"1995","journal-title":"PNAS","ISSN":"http:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"1","key":"30_16669305","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1210\/jc.82.1.147","volume":"82","author":"Husebye","year":"1997","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"http:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"3","key":"31_18727618","doi-asserted-by":"publisher","first-page":"1323","DOI":"10.1210\/jc.2004-1300","volume":"90","year":"2005","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"http:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"4","key":"32_14230518","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1111\/j.1365-2265.1986.tb01707.x","volume":"25","author":"Scherbaum","year":"1986","journal-title":"Clinical endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0300-0664","issn-type":"print"},{"issue":"6","key":"33_18939843","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1507\/endocrj.52.691","volume":"52","author":"Chan","year":"2005","journal-title":"Endocrine journal","ISSN":"http:\/\/id.crossref.org\/issn\/0918-8959","issn-type":"print"},{"issue":"6","key":"34_23718510","doi-asserted-by":"publisher","first-page":"2176","DOI":"10.1210\/jc.2006-2748","volume":"92","year":"2007","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"http:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"38","key":"35_19550258","doi-asserted-by":"publisher","first-page":"35390","DOI":"10.1074\/jbc.M102391200","volume":"276","year":"2001","journal-title":"Journal of Biological Chemistry","ISSN":"http:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"issue":"1","key":"36_38782574","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1172\/JCI44073","volume":"121","author":"Yamamoto","year":"2011","journal-title":"Journal of Clinical Investigation","ISSN":"http:\/\/id.crossref.org\/issn\/0021-9738","issn-type":"print"},{"issue":"2","key":"37_33561713","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1038\/ncb1831","volume":"11","author":"Nishiyama","year":"2009","journal-title":"Nature Cell Biology","ISSN":"http:\/\/id.crossref.org\/issn\/1476-4679","issn-type":"print"},{"issue":"6533","key":"38_15917129","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1038\/375645a0","volume":"375","author":"S\u00a0\u00a0dhof","year":"1995","journal-title":"Nature; Physical Science (London)","ISSN":"http:\/\/id.crossref.org\/issn\/1476-4687","issn-type":"print"},{"issue":"9","key":"39_15520448","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1096\/fasebj.8.9.8005390","volume":"8","author":"Bean","year":"1994","journal-title":"The FASEB Journal","ISSN":"http:\/\/id.crossref.org\/issn\/0892-6638","issn-type":"print"},{"issue":"3","key":"40_16745414","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/S0167-0115(96)02110-6","volume":"68","author":"Vallet","year":"1997","journal-title":"Regulatory peptides","ISSN":"http:\/\/id.crossref.org\/issn\/0167-0115","issn-type":"print"},{"issue":"20","key":"41_5312188","doi-asserted-by":"crossref","first-page":"12009","DOI":"10.1016\/S0021-9258(18)80167-3","volume":"264","author":"Gerdes","year":"1989","journal-title":"Journal of Biological Chemistry","ISSN":"http:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"issue":"2","key":"42_31610694","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1007\/s00441-008-0627-3","volume":"333","author":"Spiwoks-Becker","year":"2008","journal-title":"Cell and tissue research","ISSN":"http:\/\/id.crossref.org\/issn\/0302-766X","issn-type":"print"},{"issue":"14","key":"43_36911482","doi-asserted-by":"publisher","first-page":"6504","DOI":"10.1073\/pnas.1002307107","volume":"107","year":"2010","journal-title":"PNAS","ISSN":"http:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"52","key":"44_19558941","doi-asserted-by":"publisher","first-page":"50497","DOI":"10.1074\/jbc.M210146200","volume":"277","year":"2002","journal-title":"Journal of Biological Chemistry","ISSN":"http:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"key":"45_35191917","first-page":"421","volume":"31","year":"2001","ISSN":"http:\/\/id.crossref.org\/issn\/1097-4199","issn-type":"print"},{"issue":"52","key":"46_19565768","doi-asserted-by":"publisher","first-page":"52802","DOI":"10.1074\/jbc.M304727200","volume":"278","year":"2003","journal-title":"Journal of Biological Chemistry","ISSN":"http:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"issue":"32","key":"47_5720056","doi-asserted-by":"publisher","first-page":"19637","DOI":"10.1074\/jbc.272.32.19637","volume":"272","author":"Ann","year":"1997","journal-title":"Journal of Biological Chemistry","ISSN":"http:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"key":"48_33081900","first-page":"551","volume":"43","year":"2004","ISSN":"http:\/\/id.crossref.org\/issn\/1097-4199","issn-type":"print"},{"issue":"2","key":"49_17509415","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1093\/rheumatology\/keg088","volume":"42","author":"Tanaka","year":"2003","journal-title":"Rheumatology","ISSN":"http:\/\/id.crossref.org\/issn\/0080-2727","issn-type":"print"}],"container-title":["European Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/166\/3\/391.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/166\/3\/391.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:27:41Z","timestamp":1676392061000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/166\/3\/391\/6659295"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3]]},"references-count":49,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1530\/eje-11-1015","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3]]}}}