{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T13:39:35Z","timestamp":1791639575380,"version":"4.3.4"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The use of late-night salivary cortisol (LNSalC) for diagnosing subclinical hypercortisolism (SH) is debated. No data are available regarding the role of LNSalC as measured by liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) in SH diagnosis. The aim of this study was to evaluate the diagnostic accuracy of LNSalC measured by LC\u2013MS\/MS in SH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Cross-sectional prospective study of outpatients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In 70 consecutive patients with adrenal incidentalomas (AI), without signs and symptoms of hypercortisolism, we diagnosed SH in the presence of at least two of the following: cortisol after 1\u200amg overnight dexamethasone suppression test (1\u200amg DST) &gt;83\u200anmol\/l, 24-h urinary free cortisol (UFC) &gt;193\u200anmol\/24\u200ah, and morning ACTH &lt;2.2\u200apmol\/l. The LNSalC levels by LC\u2013MS\/MS at 2300\u200ah (normal values &lt;2.8\u200anmol\/l) and the presence of hypertension, type 2 diabetes mellitus (T2DM), and osteoporosis (OP) were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The increased LNSalC levels (&gt;2.8\u200anmol\/l) had an 83.3% specificity (SP) and a 31.3% sensitivity (SN) for predicting the biochemical diagnosis of SH. The increased LNSalC had an 85.2% SP and a 55.6% SN for predicting the presence of hypertension, T2DM, and OP, while the combination of LNSalC &gt;1.4\u200anmol\/l (cutoff with 100% SN) plus 1\u200amg DST &gt;50\u200anmol\/l had an 88.9% SN and an 85.2% SP (similar to SH criterion at enrollment).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In AI patients, LNSalC measured by LC\u2013MS\/MS appears to be useful in combination with 1\u200amg DST for diagnosing SH, while it is not useful as a single criterion.<\/jats:p><\/jats:sec>","DOI":"10.1530\/eje-12-0803","type":"journal-article","created":{"date-parts":[[2012,12,5]],"date-time":"2012-12-05T15:23:12Z","timestamp":1354720992000},"page":"289-296","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":50,"title":["The role of salivary cortisol measured by liquid chromatography\u2013tandem mass spectrometry in the diagnosis of subclinical hypercortisolism"],"prefix":"10.1093","volume":"168","author":[{"given":"S","family":"Palmieri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"Morelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Polledri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Fustinoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Mercadante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Olgiati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Eller Vainicher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Cairoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V V","family":"Zhukouskaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Beck-Peccoz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Chiodini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"issue":"2","key":"1_18824698","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1016\/j.ecl.2005.01.008","volume":"34","author":"Terzolo","year":"2005","journal-title":"Endocrinology and metabolism clinics of North America","ISSN":"https:\/\/id.crossref.org\/issn\/0889-8529","issn-type":"print"},{"issue":"5","key":"2_30600398","doi-asserted-by":"publisher","first-page":"1526","DOI":"10.1210\/jc.2008-0125","volume":"93","year":"2008","journal-title":"Journal of Clinical Endocrinology &amp; 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