{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:53:38Z","timestamp":1789606418352,"version":"build-2803163510"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"2","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Late diagnosis hinders proper management of small intestine neuroendocrine carcinoma (SI-NEC) patients. The olfactory receptor, family 51, subfamily E, member 1 (OR51E1) has been reported as a potential novel SI-NEC marker, without protein expression recognition. Thus, we further studied whether the encoded protein may be a novel SI-NEC clinical biomarker.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>OR51E1 coding sequence was cloned using total RNA from SI-NEC patient specimens. Quantitative real-time PCR analysis explored OR51E1 expression in laser capture microdissected SI-NEC cells and adjacent microenvironment cells. Moreover, immunohistochemistry investigated OR51E1 protein expression on operation and biopsy material from primary SI-NECs, mesentery, and liver metastases from 70 patients. Furthermore, double immunofluorescence studies explored the potential co-localization of the vesicular monoamine transporter 1 (SLC18A1, generally referred to as VMAT1) and OR51E1 in the neoplastic cells and in the intestinal mucosa adjacent to the tumor.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>OR51E1 coding sequence analysis showed absence of mutation in SI-NEC patients at different stages of disease. OR51E1 expression was higher in microdissected SI-NEC cells than in the adjacent microenvironment cells. Furthermore, both membranous and cytoplasmic OR51E1 immunostaining patterns were detected in both primary SI-NECs and metastases. Briefly, 18\/43 primary tumors, 7\/28 mesentery metastases, and 6\/18 liver metastases were \u2018positive\u2019 for OR51E1 in more than 50% of the tumor cells. In addition, co-localization studies showed that OR51E1 was expressed in &gt;50% of the VMAT1 immunoreactive tumor cells and of the enterochromaffin cells in the intestinal mucosa adjacent to the tumor.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>OR51E1 protein is a potential novel clinical tissue biomarker for SI-NECs. Moreover, we suggest its potential therapeutic molecular target development using solid tumor radioimmunotherapy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/eje-12-0814","type":"journal-article","created":{"date-parts":[[2012,11,26]],"date-time":"2012-11-26T23:37:39Z","timestamp":1353973059000},"page":"253-261","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":68,"title":["Olfactory receptor 51E1 protein as a potential novel tissue biomarker for small intestine neuroendocrine carcinomas"],"prefix":"10.1093","volume":"168","author":[{"given":"Tao","family":"Cui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apostolos V","family":"Tsolakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su-Chen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet L","family":"Cunningham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Lind","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kjell","family":"\u00d6berg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Giandomenico","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"issue":"1","key":"1_30240149","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/S1470-2045(07)70410-2","volume":"9","author":"Modlin","year":"2008","journal-title":"The lancet oncology","ISSN":"https:\/\/id.crossref.org\/issn\/1470-2045","issn-type":"print"},{"issue":"9808","key":"2_41335197","doi-asserted-by":"publisher","first-page":"2005","DOI":"10.1016\/S0140-6736(11)61742-X","volume":"378","author":"Pavel","year":"2011","journal-title":"Lancet","ISSN":"https:\/\/id.crossref.org\/issn\/0140-6736","issn-type":"print"},{"issue":"28","key":"3_35434264","doi-asserted-by":"publisher","first-page":"4656","DOI":"10.1200\/JCO.2009.22.8510","volume":"27","year":"2009","journal-title":"Journal of Clinical Oncology","ISSN":"https:\/\/id.crossref.org\/issn\/0732-183X","issn-type":"print"},{"issue":"1","key":"4_39332997","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.ecl.2010.12.003","volume":"40","author":"Kwekkeboom","year":"2011","journal-title":"Endocrinology and metabolism clinics of North America","ISSN":"https:\/\/id.crossref.org\/issn\/0889-8529","issn-type":"print"},{"key":"5_44952959","first-page":"3","volume":"30","year":"2011","journal-title":"Cancer metastasis reviews","ISSN":"https:\/\/id.crossref.org\/issn\/0167-7659","issn-type":"print"},{"issue":"5","key":"6_43212085","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1530\/ERC-11-0367","volume":"19","year":"2012","journal-title":"Endocrine-Related Cancer","ISSN":"https:\/\/id.crossref.org\/issn\/1351-0088","issn-type":"print"},{"issue":"1","key":"7_33487423","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/1479-7364-3-1-87","volume":"3","author":"Olender","year":"2008","journal-title":"Human genomics","ISSN":"https:\/\/id.crossref.org\/issn\/1473-9542","issn-type":"print"},{"key":"8_33092860","first-page":"417","volume":"48","year":"2005","ISSN":"https:\/\/id.crossref.org\/issn\/1097-4199","issn-type":"print"},{"issue":"1","key":"9_39061653","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1038\/nrd3320","volume":"10","author":"Lappano","year":"2011","journal-title":"Nature reviews. 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