{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:41:07Z","timestamp":1790052067271,"version":"4.0.1"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>To explore underlying molecular mechanisms in the pathogenesis of symptomatic sporadic primary hyperparathyroidism (PHPT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Forty-one parathyroid adenomas from patients with symptomatic PHPT and ten normal parathyroid glands either from patients with PHPT (<jats:italic>n<\/jats:italic>=3) or from euthyroid patients without PHPT during thyroid surgery (<jats:italic>n<\/jats:italic>=7) were analyzed for vitamin D receptor (VDR), calcium-sensing receptor (CASR), cyclin D1 (CD1), and parathyroid hormone (PTH) expressions. The protein expressions were assessed semiquantitatively by immunohistochemistry, based on percentage of positive cells and staining intensity, and confirmed by quantitative real-time PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunohistochemistry revealed significant reductions in VDR (both nuclear and cytoplasmic) and CASR expressions and significant increases in CD1 and PTH expressions in adenomatous compared with normal parathyroid tissue. Consistent with immunohistochemistry findings, both<jats:italic>VDR<\/jats:italic>and<jats:italic>CASR<\/jats:italic>mRNAs were reduced by 0.36- and 0.45-fold change (<jats:italic>P<\/jats:italic>&lt;0.001) and<jats:italic>CD1<\/jats:italic>and<jats:italic>PTH<\/jats:italic>mRNAs were increased by 9.4- and 17.4-fold change respectively (<jats:italic>P<\/jats:italic>&lt;0.001) in adenomatous parathyroid tissue.<jats:italic>PTH<\/jats:italic>mRNA correlated with plasma PTH (<jats:italic>r<\/jats:italic>=0.864;<jats:italic>P<\/jats:italic>&lt;0.001), but not with adenoma weight, while<jats:italic>CD1<\/jats:italic>mRNA correlated with adenoma weight (<jats:italic>r<\/jats:italic>=0.715;<jats:italic>P<\/jats:italic>&lt;0.001). There were no correlations between<jats:italic>VDR<\/jats:italic>and<jats:italic>CASR<\/jats:italic>mRNA levels and serum Ca, plasma intact PTH, or 25-hydroxyvitamin D levels. In addition, there was no relationship between the decreases in<jats:italic>VDR<\/jats:italic>and<jats:italic>CASR<\/jats:italic>mRNA expressions and the increases in<jats:italic>PTH<\/jats:italic>and<jats:italic>CD1<\/jats:italic>mRNA expressions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The expression of both VDR and CASR are reduced in symptomatic PHPT in Asian Indians. In addition,<jats:italic>CD1<\/jats:italic>expression was greatly increased and correlated with adenoma weight, implying a potential role for CD1 in adenoma growth and differential clinical expression of PHPT.<\/jats:p><\/jats:sec>","DOI":"10.1530\/eje-13-0085","type":"journal-article","created":{"date-parts":[[2013,5,10]],"date-time":"2013-05-10T01:35:40Z","timestamp":1368149740000},"page":"109-116","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":50,"title":["Simultaneous expression analysis of vitamin D receptor, calcium-sensing receptor, cyclin D1, and PTH in symptomatic primary hyperparathyroidism in Asian Indians"],"prefix":"10.1093","volume":"169","author":[{"given":"Shweta","family":"Varshney","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay Kumar","family":"Bhadada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uma Nahar","family":"Saikia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naresh","family":"Sachdeva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arunanshu","family":"Behera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashutosh Kumar","family":"Arya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadhna","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil","family":"Bhansali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ambrish","family":"Mithal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudhaker D","family":"Rao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"key":"1_29037933","first-page":"N37","volume":"17","year":"2002","journal-title":"Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research","ISSN":"https:\/\/id.crossref.org\/issn\/0884-0431","issn-type":"print"},{"issue":"4","key":"2_15917066","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1056\/NEJM199507273330407","volume":"333","author":"Brown","year":"1995","journal-title":"New England Journal of Medicine","ISSN":"https:\/\/id.crossref.org\/issn\/0028-4793","issn-type":"print"},{"issue":"4","key":"3_8483864","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1016\/0002-9343(81)90207-2","volume":"71","author":"Brown","year":"1981","journal-title":"The American journal of medicine","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9343","issn-type":"print"},{"issue":"5","key":"4_13197902","first-page":"2103","volume":"44","author":"Dokoh","year":"1984","journal-title":"Cancer Research","ISSN":"https:\/\/id.crossref.org\/issn\/0008-5472","issn-type":"print"},{"issue":"7","key":"5_12310904","doi-asserted-by":"publisher","first-page":"1673","DOI":"10.1073\/pnas.69.7.1673","volume":"69","author":"Garabedian","year":"1972","journal-title":"PNAS","ISSN":"https:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"4","key":"6_5891943","doi-asserted-by":"publisher","first-page":"1387","DOI":"10.1073\/pnas.95.4.1387","volume":"95","author":"Brenza","year":"1998","journal-title":"PNAS","ISSN":"https:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"2 Pt 2","key":"7_5979381","first-page":"S4","volume":"56","author":"DeLuca","year":"1998","journal-title":"Nutrition reviews","ISSN":"https:\/\/id.crossref.org\/issn\/0029-6643","issn-type":"print"},{"issue":"17","key":"8_9957182","doi-asserted-by":"publisher","first-page":"8097","DOI":"10.1073\/pnas.89.17.8097","volume":"89","author":"Demay","year":"1992","journal-title":"PNAS","ISSN":"https:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"6","key":"9_5260781","doi-asserted-by":"crossref","first-page":"2034","DOI":"10.1172\/JCI114114","volume":"83","author":"Arnold","year":"1989","journal-title":"Journal of Clinical Investigation","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9738","issn-type":"print"},{"issue":"5","key":"10_6004190","doi-asserted-by":"publisher","first-page":"1766","DOI":"10.1210\/jc.83.5.1766","volume":"83","author":"Palanisamy","year":"1998","journal-title":"Journal of Clinical Endocrinology &amp; 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