{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:55:18Z","timestamp":1790510118786,"version":"4.1.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"5","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Adipose tissue-derived factors link non-alcoholic fatty liver disease (NAFLD) with obesity, which has also been reported for circulating chemerin. On the other hand, hepatic chemerin and chemokine-like receptor 1 (<jats:italic>CMKLR1<\/jats:italic>) mRNA expression has not yet been studied in an extensively characterized patient collective.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>This study was cross-sectional and experimental in design.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Liver tissue samples were harvested from 47 subjects and histologically examined according to the NAFLD activity score (NAS). The concentrations of chemerin and CMKLR1 were measured using semi-quantitative real-time PCR, and the concentration of serum chemerin was measured using ELISA. To evaluate potential effects of chemerin and CMKLR1, cultured primary human hepatocytes (PHHs) were exposed to selected metabolites known to play a role in NAFLD (insulin, glucagon, palmitoic acid, and interleukin-6 (IL6)).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Chemerin and<jats:italic>CMKLR1<\/jats:italic>mRNA levels were elevated in the human liver. Their expression was correlated with the NAS (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>=0.543;<jats:italic>P<\/jats:italic>&lt;0.001 and<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>=0.355;<jats:italic>P<\/jats:italic>=0.014 respectively) and was significantly elevated in patients with definite non-alcoholic steatohepatitis (NASH) (<jats:italic>P<\/jats:italic>&lt;0.05 respectively). Linear regression analysis confirmed an independent association of liver fibrosis, steatosis, inflammation, and hepatocyte ballooning with hepatic chemerin mRNA expression (<jats:italic>P<\/jats:italic>&lt;0.05 respectively). The expression of hepatic chemerin and CMKLR1 was correlated with the measures of obesity (<jats:italic>P<\/jats:italic>&lt;0.05). The incubation of PHHs with IL6 significantly increased the expression of<jats:italic>CMKLR1<\/jats:italic>mRNA (<jats:italic>P<\/jats:italic>=0.027), while that of chemerin remained unaffected (<jats:italic>P<\/jats:italic>&gt;0.05). None of the other metabolites showed an influence (<jats:italic>P<\/jats:italic>&gt;0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This is the first study to show that chemerin mRNA expression is significantly elevated in the liver of NASH patients and that CMKLR1 expression is upregulated in liver inflammation, whereby IL6 could play a causal role.<\/jats:p><\/jats:sec>","DOI":"10.1530\/eje-13-0112","type":"journal-article","created":{"date-parts":[[2013,8,10]],"date-time":"2013-08-10T01:32:57Z","timestamp":1376098377000},"page":"547-557","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":64,"title":["Elevated hepatic chemerin mRNA expression in human non-alcoholic fatty liver disease"],"prefix":"10.1093","volume":"169","author":[{"given":"S","family":"D\u00f6cke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J F","family":"Lock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A L","family":"Birkenfeld","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Hoppe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Lieske","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Rieger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Raschzok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I M","family":"Sauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Florian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M A","family":"Osterhoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Heller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Herrmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Lindenm\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Horn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Bauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M O","family":"Weickert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Neuhaus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Stockmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"M\u00f6hlig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A F H","family":"Pfeiffer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"von Loeffelholz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"issue":"1","key":"1_32862281","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1002\/hep.22603","volume":"49","author":"Vuppalanchi","year":"2009","journal-title":"Hepatology (Baltimore, Md.)","ISSN":"https:\/\/id.crossref.org\/issn\/0270-9139","issn-type":"print"},{"key":"2_36634152","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1146\/annurev-physiol-021909-135846","volume":"72","author":"Olefsky","year":"2010","journal-title":"Annual Review of Physiology","ISSN":"https:\/\/id.crossref.org\/issn\/0066-4278","issn-type":"print"},{"issue":"4","key":"3_30811827","doi-asserted-by":"publisher","first-page":"1036","DOI":"10.1111\/j.1572-0241.2007.01709.x","volume":"103","author":"Carter-Kent","year":"2008","journal-title":"The American journal of gastroenterology","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9270","issn-type":"print"},{"key":"4_46601246","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1038\/ncpgasthep0186","volume":"2","year":"2005","journal-title":"NATURE CLINICAL PRACTICE GASTROENTEROLOGY HEPATOLOGY"},{"key":"5_46601247","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/ncpendmet0190","volume":"2","year":"2006","journal-title":"NATURE CLINICAL PRACTICE ENDOCRINOLOGY METABOLISM"},{"issue":"4","key":"6_23594184","doi-asserted-by":"publisher","first-page":"1010","DOI":"10.2337\/db06-1656","volume":"56","year":"2007","journal-title":"Diabetes","ISSN":"https:\/\/id.crossref.org\/issn\/0012-1797","issn-type":"print"},{"key":"7_46601248","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/2050-7771-1-11","volume":"1","year":"2013","journal-title":"BIOMARKER RESEARCH"},{"issue":"1","key":"8_16830170","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1111\/1523-1747.ep12276660","volume":"109","author":"Nagpal","year":"1997","journal-title":"Journal of Investigative Dermatology","ISSN":"https:\/\/id.crossref.org\/issn\/0022-202X","issn-type":"print"},{"issue":"5","key":"9_36794295","doi-asserted-by":"publisher","first-page":"1998","DOI":"10.1210\/en.2009-1098","volume":"151","year":"2010","journal-title":"Endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"5","key":"10_30536996","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1016\/j.febslet.2008.01.023","volume":"582","author":"Takahashi","year":"2008","journal-title":"FEBS Letters","ISSN":"https:\/\/id.crossref.org\/issn\/1873-3468","issn-type":"print"},{"issue":"10","key":"11_29056811","doi-asserted-by":"publisher","first-page":"4687","DOI":"10.1210\/en.2007-0175","volume":"148","year":"2007","journal-title":"Endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"3","key":"12_37491401","doi-asserted-by":"publisher","first-page":"E506","DOI":"10.1152\/ajpendo.00586.2009","volume":"299","year":"2010","journal-title":"American Journal of Physiology - Endocrinology And Metabolism","ISSN":"https:\/\/id.crossref.org\/issn\/0193-1849","issn-type":"print"},{"issue":"9","key":"13_34986188","doi-asserted-by":"publisher","first-page":"1971","DOI":"10.2337\/db08-1528","volume":"58","year":"2009","journal-title":"Diabetes","ISSN":"https:\/\/id.crossref.org\/issn\/0012-1797","issn-type":"print"},{"issue":"38","key":"14_29046646","doi-asserted-by":"publisher","first-page":"28175","DOI":"10.1074\/jbc.M700793200","volume":"282","year":"2007","journal-title":"Journal of Biological Chemistry","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"issue":"5","key":"15_41379300","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1016\/j.metabol.2011.10.008","volume":"61","author":"Chakaroun","year":"2012","journal-title":"Metabolism: clinical and experimental","ISSN":"https:\/\/id.crossref.org\/issn\/0026-0495","issn-type":"print"},{"issue":"10","key":"16_31894130","doi-asserted-by":"publisher","first-page":"2207","DOI":"10.1084\/jem.20080300","volume":"205","year":"2008","journal-title":"Journal of Experimental Medicine","ISSN":"https:\/\/id.crossref.org\/issn\/0022-1007","issn-type":"print"},{"issue":"1","key":"17_30141575","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1073\/pnas.0710487105","volume":"105","year":"2008","journal-title":"PNAS","ISSN":"https:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"7","key":"18_17846407","doi-asserted-by":"publisher","first-page":"977","DOI":"10.1084\/jem.20030382","volume":"198","year":"2003","journal-title":"Journal of Experimental Medicine","ISSN":"https:\/\/id.crossref.org\/issn\/0022-1007","issn-type":"print"},{"issue":"1","key":"19_18582235","doi-asserted-by":"crossref","first-page":"244","DOI":"10.4049\/jimmunol.174.1.244","volume":"174","author":"Zabel","year":"2005","journal-title":"The Journal of Immunology","ISSN":"https:\/\/id.crossref.org\/issn\/0022-1767","issn-type":"print"},{"issue":"4","key":"20_18697770","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1084\/jem.20041310","volume":"201","year":"2005","journal-title":"Journal of Experimental Medicine","ISSN":"https:\/\/id.crossref.org\/issn\/0022-1007","issn-type":"print"},{"issue":"2","key":"21_41334669","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.mce.2011.10.032","volume":"349","author":"Wanninger","year":"2012","journal-title":"Molecular and cellular endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0303-7207","issn-type":"print"},{"issue":"2","key":"22_41452102","doi-asserted-by":"publisher","first-page":"672","DOI":"10.1210\/en.2011-1490","volume":"153","year":"2012","journal-title":"Endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"6","key":"23_18886708","doi-asserted-by":"publisher","first-page":"1313","DOI":"10.1002\/hep.20701","volume":"41","author":"Kleiner","year":"2005","journal-title":"Hepatology (Baltimore, Md.)","ISSN":"https:\/\/id.crossref.org\/issn\/0270-9139","issn-type":"print"},{"issue":"11","key":"24_38091060","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1016\/j.tem.2010.08.001","volume":"21","author":"Ernst","year":"2010","journal-title":"Trends in endocrinology and metabolism: TEM","ISSN":"https:\/\/id.crossref.org\/issn\/1043-2760","issn-type":"print"},{"issue":"5-6","key":"25_41334711","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1016\/j.cytogfr.2011.11.004","volume":"22","author":"Bondue","year":"2011","journal-title":"Cytokine &amp; growth factor reviews","ISSN":"https:\/\/id.crossref.org\/issn\/1359-6101","issn-type":"print"},{"issue":"3","key":"26_35208484","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1111\/j.1365-2265.2009.03664.x","volume":"72","author":"Weigert","year":"2010","journal-title":"Clinical endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0300-0664","issn-type":"print"},{"issue":"8","key":"27_34918319","doi-asserted-by":"publisher","first-page":"3085","DOI":"10.1210\/jc.2008-1833","volume":"94","year":"2009","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"https:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"6","key":"28_37042772","doi-asserted-by":"publisher","first-page":"2892","DOI":"10.1210\/jc.2009-2374","volume":"95","year":"2010","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"https:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"3","key":"29_36621663","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1111\/j.1365-2362.2010.02255.x","volume":"40","author":"Ress","year":"2010","journal-title":"European journal of clinical investigation","ISSN":"https:\/\/id.crossref.org\/issn\/0014-2972","issn-type":"print"},{"key":"30_46601249","doi-asserted-by":"crossref","first-page":"235","DOI":"10.3109\/00365520903443852","volume":"45","year":"2010","journal-title":"SCANDINAVIAN JOURNAL OF GASTEROENTEROLOGY"},{"key":"31_46601250","doi-asserted-by":"crossref","first-page":"91","DOI":"10.3109\/00365521.2010.516452","volume":"46","year":"2011","journal-title":"SCANDINAVIAN JOURNAL OF GASTEROENTEROLOGY"},{"issue":"3","key":"32_32165048","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1124\/pr.108.00001","volume":"60","author":"Anderson","year":"2008","journal-title":"Pharmacological Reviews","ISSN":"https:\/\/id.crossref.org\/issn\/0031-6997","issn-type":"print"},{"key":"33_18745148","first-page":"1475","volume":"10","author":"Brunt","year":"2005","journal-title":"Frontiers in bioscience : a journal and virtual library","ISSN":"https:\/\/id.crossref.org\/issn\/1093-9946","issn-type":"print"},{"issue":"3","key":"34_22215023","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1159\/000092601","volume":"50","author":"Garcia","year":"2006","journal-title":"Annals of nutrition &amp; metabolism","ISSN":"https:\/\/id.crossref.org\/issn\/0250-6807","issn-type":"print"},{"issue":"Supplement_1","key":"35_36243106","doi-asserted-by":"publisher","first-page":"S11","DOI":"10.2337\/dc10-S011","volume":"33","author":"American Diabetes Association","year":"2010","journal-title":"Diabetes Care","ISSN":"https:\/\/id.crossref.org\/issn\/0149-5992","issn-type":"print"},{"issue":"25","key":"36_17375709","doi-asserted-by":"crossref","first-page":"3143","DOI":"10.1161\/circ.106.25.3143","volume":"106","year":"2002","journal-title":"Circulation","ISSN":"https:\/\/id.crossref.org\/issn\/0009-7322","issn-type":"print"},{"issue":"3","key":"37_36084866","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1530\/EJE-09-0737","volume":"162","year":"2010","journal-title":"European Journal of Endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0804-4643","issn-type":"print"},{"issue":"7","key":"38_13617443","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1007\/BF00280883","volume":"28","author":"Matthews","year":"1985","journal-title":"Diabetologia","ISSN":"https:\/\/id.crossref.org\/issn\/0012-186X","issn-type":"print"},{"key":"39_46601251","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1007\/BF00362995","volume":"270","year":"1960","journal-title":"PFLUGERS ARCHIV FR DIE GESAMTE PHYSIOLOGIE DES MENSCHEN UND DER TIERE"},{"issue":"6","key":"40_16047439","doi-asserted-by":"publisher","first-page":"696","DOI":"10.1016\/0168-8278(95)80226-6","volume":"22","author":"Ishak","year":"1995","journal-title":"Journal of hepatology","ISSN":"https:\/\/id.crossref.org\/issn\/0168-8278","issn-type":"print"},{"issue":"1","key":"41_14196913","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","volume":"162","author":"Chomczynski","year":"1987","journal-title":"Analytical biochemistry","ISSN":"https:\/\/id.crossref.org\/issn\/0003-2697","issn-type":"print"},{"key":"42_28904472","doi-asserted-by":"crossref","first-page":"S186","DOI":"10.1046\/j.1440-1746.17.s1.10.x","volume":"17","year":"2002","journal-title":"Journal of gastroenterology and hepatology","ISSN":"https:\/\/id.crossref.org\/issn\/0815-9319","issn-type":"print"},{"issue":"6","key":"43_10337021","doi-asserted-by":"publisher","first-page":"1117","DOI":"10.1016\/S0016-5085(00)70364-7","volume":"118","author":"RATZIU","year":"2000","journal-title":"Gastroenterology","ISSN":"https:\/\/id.crossref.org\/issn\/0016-5085","issn-type":"print"},{"issue":"5","key":"44_23368460","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1002\/hep.1840120505","volume":"12","author":"Wanless","year":"1990","journal-title":"Hepatology (Baltimore, Md.)","ISSN":"https:\/\/id.crossref.org\/issn\/0270-9139","issn-type":"print"},{"issue":"2","key":"45_33544466","first-page":"363","volume":"21","author":"Martignoni","year":"2009","journal-title":"Oncology reports","ISSN":"https:\/\/id.crossref.org\/issn\/1021-335X","issn-type":"print"},{"issue":"5","key":"46_45422477","doi-asserted-by":"publisher","first-page":"1794","DOI":"10.1210\/en.2012-2126","volume":"154","year":"2013","journal-title":"Endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"8","key":"47_22422615","doi-asserted-by":"publisher","first-page":"1106","DOI":"10.1016\/j.exphem.2006.03.011","volume":"34","author":"Zabel","year":"2006","journal-title":"Experimental hematology","ISSN":"https:\/\/id.crossref.org\/issn\/0301-472X","issn-type":"print"}],"container-title":["European Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/169\/5\/547.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/169\/5\/547.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:29:18Z","timestamp":1676392158000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/169\/5\/547\/6661774"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11]]},"references-count":47,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.1530\/eje-13-0112","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11]]}}}