{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T20:42:11Z","timestamp":1770324131738,"version":"3.49.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:sec>\n<jats:title>Objective<\/jats:title>\n<jats:p>Pregnancy-associated plasma protein-A2 (PAPP-A2) is a metalloproteinase that cleaves IGFBP-3 and IGFBP-5. Human mutations in <jats:italic>PAPPA2<\/jats:italic> result in short stature with a low percentage of free IGF-I. Little is known about PAPP-A2 levels and the regulation of free IGF-I throughout childhood. We examined PAPP-A2 and intact IGFBP-3 levels in childhood and explored associations between PAPP-A2, free and total IGF-I, and total and intact IGFBP-3 and their relationship to the percentage of free to total IGF-I and anthropometric factors.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Design<\/jats:title>\n<jats:p>Cross-sectional study at a single center.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>PAPP-A2, free IGF-I, and intact IGFBP-3 levels were measured in childhood (3\u201318 years old) and an evaluation of the relationship between these proteins and anthropometric factors.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>In 838 children, PAPP-A2 consistently decreased throughout childhood. In contrast, free IGF-I increased. A pubertal peak in free IGF-I was present in females but was less evident in males. Intact and total IGFBP-3 increased throughout childhood; however, intact IGFBP-3 had a more marked rise than total IGFBP-3. Percent free IGF-I decreased with no distinct pubertal peak. PAPP-A2 levels positively correlated with the percent free IGF-I (Male, Female; <jats:italic>r<\/jats:italic>\u2009=\u20090.18, 0.38; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) and negatively with intact IGFBP-3 (Male, Female; <jats:italic>r<\/jats:italic>\u2009=\u2009\u22120.58, \u22120.65; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001).<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>This is the first study to describe serum PAPP-A2 and intact IGFBP-3 in children between 3 and 18 years of age. Our correlative findings suggest that PAPP-A2 is an important regulator of the percent free IGF-I which can be a marker of perturbations in the GH\/IGF-I axis.<\/jats:p>\n<\/jats:sec>","DOI":"10.1530\/eje-19-0859","type":"journal-article","created":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T16:57:08Z","timestamp":1579625828000},"page":"363-374","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":17,"title":["Anthropometric and biochemical correlates of PAPP-A2, free IGF-I, and IGFBP-3 in childhood"],"prefix":"10.1093","volume":"182","author":[{"given":"Masanobu","family":"Fujimoto","sequence":"first","affiliation":[{"name":"1Cincinnati Center for Growth Disorders, Division of Endocrinology, Cincinnati Children\u2019s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA"},{"name":"2Division of Pediatrics and Perinatology, Tottori University Faculty of Medicine, Yonago, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane C","family":"Khoury","sequence":"additional","affiliation":[{"name":"1Cincinnati Center for Growth Disorders, Division of Endocrinology, Cincinnati Children\u2019s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA"},{"name":"3Division of Biostatistics and Epidemiology, Cincinnati Children\u2019s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip R","family":"Khoury","sequence":"additional","affiliation":[{"name":"4Heart Institute Research Core, Cincinnati Children\u2019s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhanu","family":"Kalra","sequence":"additional","affiliation":[{"name":"5Ansh Labs, Webster, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ajay","family":"Kumar","sequence":"additional","affiliation":[{"name":"5Ansh Labs, Webster, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Sluss","sequence":"additional","affiliation":[{"name":"5Ansh Labs, Webster, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus","family":"Oxvig","sequence":"additional","affiliation":[{"name":"6Department of Molecular Biology and Genetics, Aarhus University, Aarhus C, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivian","family":"Hwa","sequence":"additional","affiliation":[{"name":"1Cincinnati Center for Growth Disorders, Division of Endocrinology, Cincinnati Children\u2019s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Dauber","sequence":"additional","affiliation":[{"name":"1Cincinnati Center for Growth Disorders, Division of Endocrinology, Cincinnati Children\u2019s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA"},{"name":"7Division of Endocrinology, Children\u2019s National Hospital, Washington, District of Columbia, USA"},{"name":"8Department of Pediatrics, George Washington University School of Medicine and Health Sciences, Washington, District of Columbia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["European Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/182\/3\/EJE-19-0859.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/182\/3\/EJE-19-0859.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:14:08Z","timestamp":1676391248000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/182\/3\/363\/6653938"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1530\/eje-19-0859","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3]]}}}