{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T00:32:17Z","timestamp":1793320337796,"version":"4.5.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:sec>\n<jats:title>Objective<\/jats:title>\n<jats:p>Liquid chromatography coupled to tandem mass spectrometry (LC-MS\/MS) has become state of the art for the quantitative analysis of steroid hormones. Although method comparisons show that aldosterone measurement using LC-MS\/MS yields considerably lower levels than immunoassays (IAs), method-specific cutoff values for primary aldosteronism (PA) are largely missing. Objective of this study was to analyze the diagnostic accuracy of proposed LC-MS\/MS-specific cutoff values for the saline infusion test (SIT).<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Design and methods<\/jats:title>\n<jats:p>From 2016 to 2019, 104 consecutive patients suspected of PA underwent the SIT and captopril challenge test in the tertiary medical center at the University Hospital of Leipzig, Germany. Patients with positive case confirmation underwent adrenal imaging and adrenal venous sampling for subtype classification.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Overall, proposed assay-specific PAC<jats:sub>LC-MS\/MS<\/jats:sub> cutoff values for the SIT achieved higher diagnostic accuracy than established PAC<jats:sub>IA<\/jats:sub> values with a sensitivity and specificity of 87.5% (95% CI: 71.0\u201396.5) and 97% (95% CI: 89.6\u201399.6) for a cutoff of 120 pmol\/L and 93.8% (95% CI: 79.2\u201399.2) and 92.5% (95% CI: 83.4\u201397.5) for a cutoff of 94 pmol\/L. The most accurate post-SIT PAC<jats:sub>LC-MS\/MS<\/jats:sub> cutoff value in this study was 83 pmol\/L, yielding a sensitivity and specificity of 96.9% (95% CI: 83.8\u201399.9) and 92.5% (95% CI: 83.4\u201397.5), respectively.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>The present data confirm the need for the implication of lower method-specific aldosterone cutoff values for the diagnosis of PA with LC-MS\/MS based aldosterone measurement.<\/jats:p>\n<\/jats:sec>","DOI":"10.1530\/eje-20-0030","type":"journal-article","created":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T15:24:03Z","timestamp":1590593043000},"page":"191-201","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":16,"title":["Prospective evaluation of aldosterone LC-MS\/MS-specific cutoffs for the saline infusion test"],"prefix":"10.1093","volume":"183","author":[{"given":"Charlotte Michaela","family":"Fries","sequence":"first","affiliation":[{"name":"1Medical Department III, Endocrinology, Nephrology, Rheumatology, University Hospital of Leipzig, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon Ju","family":"Bae","sequence":"additional","affiliation":[{"name":"2Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University of Leipzig, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nada","family":"Rayes","sequence":"additional","affiliation":[{"name":"3Endocrine Surgery, Department for Visceral, Transplant, Thoracic and Vascular Surgery, University Hospital of Leipzig, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Sandner","sequence":"additional","affiliation":[{"name":"1Medical Department III, Endocrinology, Nephrology, Rheumatology, University Hospital of Leipzig, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berend","family":"Isermann","sequence":"additional","affiliation":[{"name":"2Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University of Leipzig, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Stumvoll","sequence":"additional","affiliation":[{"name":"1Medical Department III, Endocrinology, Nephrology, Rheumatology, University Hospital of Leipzig, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Fagotto","sequence":"additional","affiliation":[{"name":"4Department of Medicine IV, Klinikum der Universit\u00e4t, Ludwig-Maximilians-Universit\u00e4t, M\u00fcnchen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Reincke","sequence":"additional","affiliation":[{"name":"4Department of Medicine IV, Klinikum der Universit\u00e4t, Ludwig-Maximilians-Universit\u00e4t, M\u00fcnchen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Bidlingmaier","sequence":"additional","affiliation":[{"name":"4Department of Medicine IV, Klinikum der Universit\u00e4t, Ludwig-Maximilians-Universit\u00e4t, M\u00fcnchen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vogel","family":"Mandy","sequence":"additional","affiliation":[{"name":"5Leipzig Research Center for Civilization Diseases (LIFE Child), University of Leipzig, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Kratzsch","sequence":"additional","affiliation":[{"name":"2Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University of Leipzig, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiebke Kristin","family":"Fenske","sequence":"additional","affiliation":[{"name":"1Medical Department III, Endocrinology, Nephrology, Rheumatology, University Hospital of Leipzig, Leipzig, Germany"},{"name":"6Integrated Research and Treatment Centre for Adiposity Diseases, Department of Medicine, University of Leipzig, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","container-title":["European Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/183\/2\/EJE-20-0030.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/183\/2\/EJE-20-0030.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:14:26Z","timestamp":1676391266000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/183\/2\/191\/6653755"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1530\/eje-20-0030","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8]]}}}