{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T06:54:16Z","timestamp":1676962456802},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,7,1]]},"abstract":"<jats:sec>\n<jats:title>Objective<\/jats:title>\n<jats:p>Autoimmune Addison\u2019s disease (AD) results from a combination of the genetic predisposition, unclear environmental triggers and ensuing immune dysfunction. MicroRNA molecules (miRNAs) are involved in post-transcriptional regulation of numerous target genes, hence may affect the immune function and promote autoimmunity. A deregulated miRNAs profile was reported in several autoimmune conditions. Our study was aimed at a global analysis of miRNA expression in CD4+ T cells from patients with AD.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>CD4+ T cells were separated from peripheral blood, total RNA enriched in miRNAs extracted, and miRNA expression determined by small RNA sequencing. Global miRNA was investigated in 11 AD subjects and 9 age-matched healthy controls, with subsequent validation of the differentially expressed miRNAs by RT-qPCR in 29 patients and 28 controls.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>The analysis revealed upregulation of 9 miRNAs and downregulation of miR-509-3p in CD4+ T cells from patients with AD (cut-off fold change (FC) &gt;2, Benjamini\u2013Hochberg <jats:italic>P<\/jats:italic>\u2009 &lt; 0.05). RT-qPCR validation confirmed overexpression of miR-7977 (<jats:italic>P<\/jats:italic>\u2009&lt; 0.0001, FC\u2009=\u20092.7), miR-374a-5p and miR-1260b (<jats:italic>P<\/jats:italic>\u2009&lt; 0.05, FC\u2009=\u20091.3 and 1.2, respectively). miR-7977 was upregulated in patients with coexisting autoimmune conditions vs those with isolated AD (<jats:italic>P<\/jats:italic>\u2009= 0.005, mean FC\u2009=\u20092.2). Moreover, miR-7977 abundance appeared correlated with the number of autoimmune comorbidities (<jats:italic>P<\/jats:italic>\u2009&lt;0.0001, r\u2009=\u20090.736) and serum autoantibodies against thyroid peroxidase (<jats:italic>P<\/jats:italic>\u2009&lt; 0.001, r\u2009=\u20090.588).<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>Our study demonstrates upregulated expression of miR-7977 in CD4+ T cells from patients with AD, especially with its polyendocrine form. Further analyses are warranted to replicate our results, establish the marker utility of miR-7977, and elucidate its functional role in autoimmunity.<\/jats:p>\n<\/jats:sec>","DOI":"10.1530\/eje-21-0007","type":"journal-article","created":{"date-parts":[[2021,5,7]],"date-time":"2021-05-07T13:29:03Z","timestamp":1620394143000},"page":"145-154","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-7","source":"Crossref","is-referenced-by-count":2,"title":["Overexpression of miR-7977 in CD4+ T cells is associated with multiplex autoimmunity in patients with Addison\u2019s disease"],"prefix":"10.1093","volume":"185","author":[{"given":"Marta","family":"Fichna","sequence":"first","affiliation":[{"name":"1Department of Endocrinology, Metabolism and Internal Medicine, Pozna\u0144 University of Medical Sciences, Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magdalena","family":"\u017burawek","sequence":"additional","affiliation":[{"name":"2Institute of Human Genetics, Polish Academy of Sciences, Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jakub","family":"P Fichna","sequence":"additional","affiliation":[{"name":"3Department of Neurodegenerative Disorders, Mossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marek","family":"Rucha\u0142a","sequence":"additional","affiliation":[{"name":"1Department of Endocrinology, Metabolism and Internal Medicine, Pozna\u0144 University of Medical Sciences, Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","container-title":["European Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/185\/1\/EJE-21-0007.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/downloadpdf\/journals\/eje\/185\/1\/EJE-21-0007.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:16:15Z","timestamp":1676391375000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/185\/1\/145\/6654381"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,1]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/eje-21-0007","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,1]]}}}