{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:20:22Z","timestamp":1791829222816,"version":"4.3.4"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"4","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,10]]},"abstract":"<jats:p>Moln\u00e1r M, Hertelendy F. Signal transduction in rat myometrial cells: comparison of the actions of endothelin-1, oxytocin and prostaglandin F<jats:sub>2\u03b1<\/jats:sub>. Eur J Endocrinol 1995;133:467\u201374. ISSN 0804\u20134643<\/jats:p>\n        <jats:p>The objectives of this study were to evaluate and compare the actions of endothelin-1 (ET-1), oxytocin, prostaglandin F<jats:sub>2\u03b1<\/jats:sub> (PGF<jats:sub>2\u03b1<\/jats:sub>) and inositol 1,4,5-trisphosphate (IP<jats:sub>3<\/jats:sub>) on Ca<jats:sup>2+<\/jats:sup> mobilization in permeabilized rat myometrial cells and to examine the activation of the inositol lipid cycle in intact myocytes. Cells were isolated from late pregnant rat myometrium and used as confluent monolayers after a single passage. All four agonists caused a biphasic release of<jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup> from non-mitochondrial pool(s), with the rank order of potency: oxytocin &gt; PGF<jats:sub>2\u03b1<\/jats:sub> &gt; ET-1 &gt; IP<jats:sub>3<\/jats:sub>. Inhibitors of phospholipase C blocked ET-1-and oxytocin-promoted but not PGF<jats:sub>2\u03b1<\/jats:sub>-promoted <jats:sup>45<\/jats:sup>Ca<jats:sub>2+<\/jats:sub> efflux. Similarly, heparin, an IP<jats:sub>3<\/jats:sub> receptor blocker, failed to inhibit PGF<jats:sub>2\u03b1<\/jats:sub>-induced Ca<jats:sub>2+<\/jats:sub> release while inhibiting the action of the other agonists. Endothelin-1 and oxytocin stimulated inositol phosphate accumulation at concentrations similar to those that promoted <jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup> efflux, whereas about 100 times higher concentrations of PGF<jats:sub>2\u03b1<\/jats:sub> were needed to activate this signaling pathway in intact cells. It is concluded that the primary action of PGF<jats:sub>2\u03b1<\/jats:sub> in myometrial cells is to enhance Ca<jats:sup>2+<\/jats:sup> influx, whereas oxytocin and ET-1 receptors are coupled to phospholipase C, generating IP<jats:sub>3<\/jats:sub> and raising the intracellular concentration of free Ca<jats:sup>2+<\/jats:sup> from intracellular as well as extracellular sources.<\/jats:p>\n        <jats:p>Frank Hertelendy, Dept. Ob\/Gyn, St Loufis University Health Sciences Center, 3635 Vista Ave at Grand Blvd, St Louis, MO 63110-0250, USA<\/jats:p>","DOI":"10.1530\/eje.0.1330467","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T22:12:49Z","timestamp":1229379169000},"page":"467-474","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":27,"title":["Signal transduction in rat myometrial cells: comparison of the actions of endothelin-1, oxytocin and prostaglandin F2\u03b1"],"prefix":"10.1093","volume":"133","author":[{"given":"Mikl\u00f3s","family":"Moln\u00e1r","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Hertelendy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","container-title":["European Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/133\/4\/eje_133_4_015.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/133\/4\/eje_133_4_015.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:21:10Z","timestamp":1676391670000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/133\/4\/467\/6754519"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,10]]},"references-count":0,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1530\/eje.0.1330467","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,10]]}}}