{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:22:16Z","timestamp":1792113736846,"version":"4.4.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n        <jats:p><jats:italic>Objective<\/jats:italic>: Human islet amyloid polypeptide (hIAPP), also named amylin, is a pancreatic \u03b2 cell protein implicated in the pathogenesis of pancreatic islet amyloid formation and type 2 diabetes mellitus. To study the (patho)physiological roles of hIAPP, we have generated transgenic mice that overexpress hIAPP mRNA, in relation to endogenous mouse IAPP (mIAPP) mRNA, in pancreatic \u03b2 cells. The biological activity of human and mouse IAPP derived from pancreatic extracts was determined.<\/jats:p>\n        <jats:p><jats:italic>Methods<\/jats:italic>: Pancreatic and plasma extracts of transgenic and control mice were analyzed by reversedphase high-performance liquid chromatography (HPLC) and radioimmunoassay, yielding a separation of hIAPP from mIAPP. Biological activity of immunoreactive human and mouse IAPP components derived from pancreatic extracts was assessed by calcitonin receptor-mediated stimulation of cyclic AMP accumulation in T47D human breast carcinoma cells.<\/jats:p>\n        <jats:p><jats:italic>Results<\/jats:italic>: The predominant immunoreactive human and mouse IAPP gene products had the retention times on HPLC analysis of the corresponding synthetic peptides. The ratio of bioactive over immunoreactive hIAPP and mIAPP was 0\u00b793 \u00b10\u00b718 and 1\u00b719 \u00b10\u00b756 respectively. In extracts of two plasma pools from 4 transgenic animals, hIAPP was 4\u00b76- to 7-fold more abundant than mIAPP.<\/jats:p>\n        <jats:p><jats:italic>Conclusion<\/jats:italic>; This study has shown that correctly processed hIAPP produced in transgenic mouse pancreatic \u03b2 cells exhibits full biological activity. The results validate these transgenic mice for the study of (patho)physiological roles of hIAPP <jats:italic>in vivo<\/jats:italic>.<\/jats:p>\n        <jats:p><jats:italic>European Journal of Endocrinology<\/jats:italic> <jats:bold>136<\/jats:bold> 107\u2013113<\/jats:p>","DOI":"10.1530\/eje.0.1360107","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T22:12:49Z","timestamp":1229379169000},"page":"107-113","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":8,"title":["Biologically active human islet amyloid polypeptide\/amylin in transgenic mice"],"prefix":"10.1093","volume":"136","author":[{"given":"Karen L","family":"van Hulst","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter","family":"Born","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Muff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cor","family":"Oosterwijk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marinus A","family":"Blankenstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelis JM","family":"Lips","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan A","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo WM","family":"H\u00f6ppener","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","container-title":["European Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/136\/1\/eje_136_1_021.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/136\/1\/eje_136_1_021.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T16:23:15Z","timestamp":1676391795000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/136\/1\/107\/6753646"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,1]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/eje.0.1360107","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,1]]}}}