{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:17:02Z","timestamp":1761707822879},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"4","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":["eur j endocrinol"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>: Androgens are critical for the development and maintenance of male sexual characteristics. Their action is mediated through the androgen receptor (AR). Ligand-bound AR interacts with coactivator proteins that mediate transcriptional activation. Such coactivators include three members of the 160 kDa proteins (p160s): SRC-1, TIF2\/GRIP1, and p\/CIP\/RAC3\/ACTR\/AIB1\/TRAM-1. The aim of this study was to investigate the expression of the three p160 coactivators and their association with AR in testis and epididymis.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>: We determined the localization of these three p160 coactivators in immature and mature rat testis, and epididymis by immunohistochemistry using the specific monoclonal antibodies. We also performed double immunofluorescence staining to examine whether p160s are colocalized with AR in these tissues.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>: In seminiferous tubules of mature rat testis, SRC-1 and TRAM-1 immunoreactivity was found predominantly in spermatogonia and spermatocytes. In contrast, TIF2 was expressed predominantly in Sertoli cells. AR was coexpressed with TIF2 in this cell type. In immature rat testis, however, all three coactivators were expressed in both germ cells and Sertoli cells. In the epididymis, SRC-1 and TIF2 immunoreactivities were localized in nuclei of epithelial cells. However, TRAM-1 immunostaining was observed in the luminal portion of the cytoplasm with greater intensity than in the nucleus, especially in the caput epididymidis.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>: The cell-type-specific expression of p160 coactivators suggests specific roles in male reproductive organs. Further, the strong cytoplasmic localization of TRAM-1 protein in epithelial cells of epididymis suggests that TRAM-1 may have additional role(s) in transcriptional regulation.<\/jats:p>","DOI":"10.1530\/eje.1.01990","type":"journal-article","created":{"date-parts":[[2005,9,27]],"date-time":"2005-09-27T16:52:45Z","timestamp":1127839965000},"page":"595-604","update-policy":"http:\/\/dx.doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":9,"title":["Differential expression of p160 steroid receptor coactivators in the rat testis and epididymis"],"prefix":"10.1093","volume":"153","author":[{"given":"Junko","family":"Igarashi-Migitaka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Takeshita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriyuki","family":"Koibuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shozo","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ritsuko","family":"Ohtani-Kaneko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuaki","family":"Hirata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","reference":[{"issue":"12","key":"1_5124296","doi-asserted-by":"publisher","first-page":"1265","DOI":"10.1210\/mend-2-12-1265","volume":"2","author":"Lubahn","year":"1988","journal-title":"Molecular Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0888-8809","issn-type":"print"},{"issue":"4850","key":"2_4881419","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1126\/science.3353726","volume":"240","author":"Chang","year":"1988","journal-title":"Science","ISSN":"http:\/\/id.crossref.org\/issn\/0036-8075","issn-type":"print"},{"issue":"2","key":"3_16999995","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1210\/er.23.2.175","volume":"23","author":"Heinlein","year":"2002","journal-title":"Endocrine Reviews","ISSN":"http:\/\/id.crossref.org\/issn\/0163-769X","issn-type":"print"},{"issue":"5240","key":"4_16096224","doi-asserted-by":"publisher","first-page":"1354","DOI":"10.1126\/science.270.5240.1354","volume":"270","author":"O\u00a0ate","year":"1995","journal-title":"Science","ISSN":"http:\/\/id.crossref.org\/issn\/0036-8075","issn-type":"print"},{"issue":"3","key":"5_5705763","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1016\/S0092-8674(00)80516-4","volume":"90","author":"Chen","year":"1997","journal-title":"Cell","ISSN":"http:\/\/id.crossref.org\/issn\/0092-8674","issn-type":"print"},{"issue":"5328","key":"6_16876852","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1126\/science.277.5328.965","volume":"277","author":"Anzick","year":"1997","journal-title":"Science","ISSN":"http:\/\/id.crossref.org\/issn\/0036-8075","issn-type":"print"},{"issue":"14","key":"7_23311197","doi-asserted-by":"crossref","first-page":"3667","DOI":"10.1002\/j.1460-2075.1996.tb00736.x","volume":"15","author":"Voegel","year":"1996","journal-title":"The EMBO Journal","ISSN":"http:\/\/id.crossref.org\/issn\/0261-4189","issn-type":"print"},{"issue":"8","key":"8_16293871","doi-asserted-by":"publisher","first-page":"3594","DOI":"10.1210\/en.137.8.3594","volume":"137","author":"Takeshita","year":"1996","journal-title":"Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"3","key":"9_16208642","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0092-8674(00)81118-6","volume":"85","author":"Kamei","year":"1996","journal-title":"Cell","ISSN":"http:\/\/id.crossref.org\/issn\/0092-8674","issn-type":"print"},{"issue":"10","key":"10_16216327","doi-asserted-by":"publisher","first-page":"4948","DOI":"10.1073\/pnas.93.10.4948","volume":"93","author":"Hong","year":"1996","journal-title":"PNAS","ISSN":"http:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"16","key":"11_16862847","doi-asserted-by":"publisher","first-page":"8479","DOI":"10.1073\/pnas.94.16.8479","volume":"94","author":"Li","year":"1997","journal-title":"PNAS","ISSN":"http:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"6634","key":"12_16817950","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1038\/42652","volume":"387","author":"Torchia","year":"1997","journal-title":"Nature; Physical Science (London)","ISSN":"http:\/\/id.crossref.org\/issn\/1476-4687","issn-type":"print"},{"issue":"44","key":"13_5778585","doi-asserted-by":"publisher","first-page":"27629","DOI":"10.1074\/jbc.272.44.27629","volume":"272","author":"Takeshita","year":"1997","journal-title":"Journal of Biological Chemistry","ISSN":"http:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"issue":"2","key":"14_19547623","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1074\/jbc.M004228200","volume":"276","year":"2001","journal-title":"Journal of Biological Chemistry","ISSN":"http:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"issue":"5358","key":"15_5932820","doi-asserted-by":"publisher","first-page":"1922","DOI":"10.1126\/science.279.5358.1922","volume":"279","author":"Xu","year":"1998","journal-title":"Science","ISSN":"http:\/\/id.crossref.org\/issn\/0036-8075","issn-type":"print"},{"issue":"1","key":"16_17464111","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1523\/JNEUROSCI.23-01-00213.2003","volume":"23","author":"Nishihara","year":"2003","journal-title":"Journal of Neuroscience","ISSN":"http:\/\/id.crossref.org\/issn\/0270-6474","issn-type":"print"},{"issue":"16","key":"17_17163196","doi-asserted-by":"publisher","first-page":"5923","DOI":"10.1128\/MCB.22.16.5923-5937.2002","volume":"22","author":"Gehin","year":"2002","journal-title":"Molecular and Cellular Biology","ISSN":"http:\/\/id.crossref.org\/issn\/0270-7306","issn-type":"print"},{"issue":"12","key":"18_19669620","doi-asserted-by":"publisher","first-page":"6379","DOI":"10.1073\/pnas.120166297","volume":"97","year":"2000","journal-title":"PNAS","ISSN":"http:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"25","key":"19_19670374","doi-asserted-by":"publisher","first-page":"13549","DOI":"10.1073\/pnas.260463097","volume":"97","year":"2000","journal-title":"PNAS","ISSN":"http:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"22","key":"20_17082661","doi-asserted-by":"publisher","first-page":"2653","DOI":"10.1161\/01.CIR.0000018947.95555.65","volume":"105","year":"2002","journal-title":"Circulation","ISSN":"http:\/\/id.crossref.org\/issn\/0009-7322","issn-type":"print"},{"issue":"3","key":"21_11154167","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1055\/s-2001-14938","volume":"33","author":"Glaeser","year":"2001","journal-title":"Hormone and metabolic research. Hormon- und Stoffwechselforschung. Hormones et m&#x00A0;&#x00A0;tabolisme","ISSN":"http:\/\/id.crossref.org\/issn\/0018-5043","issn-type":"print"},{"issue":"2","key":"22_10731020","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/S0002-9440(10)65298-4","volume":"154","author":"Ghadimi","year":"1999","journal-title":"American Journal Of Pathology","ISSN":"http:\/\/id.crossref.org\/issn\/0002-9440","issn-type":"print"},{"issue":"18","key":"23_18447883","doi-asserted-by":"publisher","first-page":"6134","DOI":"10.1158\/1078-0432.CCR-04-0561","volume":"10","author":"Henke","year":"2004","journal-title":"Clinical Cancer Research","ISSN":"http:\/\/id.crossref.org\/issn\/1078-0432","issn-type":"print"},{"key":"24_25994548","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1002\/1097-0215(20000520)89:3<217::AID-IJC2>3.0.CO;2-6","volume":"89","year":"2000","journal-title":"INTERNATIONAL JOURNAL OF CANCER"},{"issue":"12","key":"25_11444839","doi-asserted-by":"publisher","first-page":"1928","DOI":"10.1054\/bjoc.2001.2179","volume":"85","author":"Gnanapragasam","year":"2001","journal-title":"British Journal of Cancer","ISSN":"http:\/\/id.crossref.org\/issn\/1532-1827","issn-type":"print"},{"issue":"6","key":"26_17087466","doi-asserted-by":"publisher","first-page":"2960","DOI":"10.1210\/jc.87.6.2960","volume":"87","author":"Gregory","year":"2002","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"http:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"3","key":"27_17501823","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1210\/en.2002-220726","volume":"144","author":"Doisneau-Sixou","year":"2003","journal-title":"Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"6","key":"28_9641705","doi-asserted-by":"publisher","first-page":"3187","DOI":"10.1210\/endo-129-6-3187","volume":"129","author":"PRINS","year":"1991","journal-title":"Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"1","key":"29_10713034","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1210\/jc.84.1.350","volume":"84","author":"Suarez-Quian","year":"1999","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"http:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"6","key":"30_17299917","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1002\/j.1939-4640.2002.tb02345.x","volume":"23","author":"Zhou","year":"2002","journal-title":"Journal of Andrology","ISSN":"http:\/\/id.crossref.org\/issn\/0196-3635","issn-type":"print"},{"issue":"5","key":"31_16754707","doi-asserted-by":"crossref","first-page":"2735","DOI":"10.1128\/MCB.17.5.2735","volume":"17","author":"Hong","year":"1997","journal-title":"Molecular and Cellular Biology","ISSN":"http:\/\/id.crossref.org\/issn\/0270-7306","issn-type":"print"},{"issue":"11","key":"32_11178595","first-page":"4315","volume":"61","author":"Gregory","year":"2001","journal-title":"Cancer Research","ISSN":"http:\/\/id.crossref.org\/issn\/0008-5472","issn-type":"print"},{"issue":"6","key":"33_9240054","doi-asserted-by":"publisher","first-page":"3180","DOI":"10.1210\/endo-127-6-3180","volume":"127","author":"SAR","year":"1990","journal-title":"Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"1","key":"34_9129204","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1677\/joe.0.1260017","volume":"126","author":"Takeda","year":"1990","journal-title":"Journal of Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0022-0795","issn-type":"print"},{"issue":"3","key":"35_15588813","doi-asserted-by":"publisher","first-page":"1227","DOI":"10.1210\/en.135.3.1227","volume":"135","author":"Bremner","year":"1994","journal-title":"Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"5","key":"36_15464378","doi-asserted-by":"publisher","first-page":"2307","DOI":"10.1210\/en.134.5.2307","volume":"134","author":"Vornberger","year":"1994","journal-title":"Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"9","key":"37_17783838","doi-asserted-by":"publisher","first-page":"1681","DOI":"10.1210\/me.2003-0116","volume":"17","year":"2003","journal-title":"Molecular Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0888-8809","issn-type":"print"},{"issue":"11","key":"38_10845293","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1172\/JCI4289","volume":"103","author":"Ghadessy","year":"1999","journal-title":"Journal of Clinical Investigation","ISSN":"http:\/\/id.crossref.org\/issn\/0021-9738","issn-type":"print"},{"issue":"8","key":"39_10423239","doi-asserted-by":"publisher","first-page":"1187","DOI":"10.1210\/me.14.8.1187","volume":"14","author":"Lim","year":"2000","journal-title":"Molecular Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0888-8809","issn-type":"print"},{"issue":"13","key":"40_19685467","doi-asserted-by":"publisher","first-page":"4453","DOI":"10.1073\/pnas.0400234101","volume":"101","year":"2004","journal-title":"PNAS","ISSN":"http:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"5","key":"41_10305680","doi-asserted-by":"publisher","first-page":"1693","DOI":"10.1210\/en.141.5.1693","volume":"141","author":"de Arrieta","year":"2000","journal-title":"Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"4","key":"42_10766419","doi-asserted-by":"publisher","first-page":"1957","DOI":"10.1210\/en.140.4.1957","volume":"140","author":"Misiti","year":"1999","journal-title":"Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"5","key":"43_11132165","doi-asserted-by":"publisher","first-page":"783","DOI":"10.1210\/me.15.5.783","volume":"15","author":"Chen","year":"2001","journal-title":"Molecular Endocrinology","ISSN":"http:\/\/id.crossref.org\/issn\/0888-8809","issn-type":"print"},{"issue":"18","key":"44_17205617","doi-asserted-by":"publisher","first-page":"6611","DOI":"10.1128\/MCB.22.18.6611-6626.2002","volume":"22","author":"Qutob","year":"2002","journal-title":"Molecular and Cellular Biology","ISSN":"http:\/\/id.crossref.org\/issn\/0270-7306","issn-type":"print"},{"issue":"34","key":"45_19563107","doi-asserted-by":"publisher","first-page":"32195","DOI":"10.1074\/jbc.M300730200","volume":"278","year":"2003","journal-title":"Journal of Biological Chemistry","ISSN":"http:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"}],"container-title":["European Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/153\/4\/1530595.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eje.bioscientifica.com\/view\/journals\/eje\/153\/4\/1530595.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,30]],"date-time":"2024-01-30T10:54:49Z","timestamp":1706612089000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejendo\/article\/153\/4\/595\/6694688"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,10]]},"references-count":45,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1530\/eje.1.01990","relation":{},"ISSN":["0804-4643","1479-683X"],"issn-type":[{"value":"0804-4643","type":"print"},{"value":"1479-683X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,10]]}}}