{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T22:04:46Z","timestamp":1783634686685,"version":"3.55.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"4","content-domain":{"domain":["eje.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":["eur j endocrinol"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:p>We have recently demonstrated that the antiproliferative activity of GnRH-II on human endometrial and ovarian cancer cell lines is not mediated through the GnRH-I receptor. A functional receptor for human GnRH-II has not yet been identified. In this study, we have generated a polyclonal antiserum to the putative human GnRH-II receptor using a peptide (YSPTMLTEVPPC) corresponding to the third extracellular domain coupled to keyhole limpet haemocyanin via the Cys residue. A database search showed no identical peptide sequences in any other human gene. To avoid cross-reactions against two similar amino acid sequences the antiserum was pre-absorbed using these peptides. Immune histological sections of human placenta and human endometrial, ovarian and prostate cancers using rabbit anti-human GnRH-II receptor antiserum showed GnRH-II receptor-like staining. Western blot analysis of cell membrane preparations of human endometrial and ovarian cancer cell lines yielded a band at approximately 43 kDa whereas Western blot analysis of cell membrane preparations of ovaries obtained from the marmoset monkey (<jats:italic>Callithrix jacchus<\/jats:italic>) yielded a band at approximately 54 kDa. To identify the GnRH-II receptor-like antigen we used the photo-affinity labelling technique. Photochemical reaction of <jats:sup>125<\/jats:sup>I-labelled (4-azidobenzoyl)-N-hydroxysuccinimide-[<jats:sc>d<\/jats:sc>-Lys<jats:sup>6<\/jats:sup>]-GnRH-II (10<jats:sup>\u22129<\/jats:sup> M) with cell membrane preparations of human endometrial and ovarian cancer cells yielded a band at approximately 43 kDa. In competition experiments, the GnRH-I agonist Triptorelin (10<jats:sup>\u22127<\/jats:sup> M) showed a weak decrease of <jats:sup>125<\/jats:sup>I-labelled (4-azidobenzoyl)-N-hydroxysuccinimide-[<jats:sc>d<\/jats:sc>-Lys<jats:sup>6<\/jats:sup>]-GnRH-II binding to its binding site. The GnRH-I antagonist Cetrorelix (10<jats:sup>\u22127<\/jats:sup> M) showed a clearly stronger decrease, whereas GnRH-II agonist [<jats:sc>d<\/jats:sc>-Lys<jats:sup>6<\/jats:sup>]-GnRH-II (10<jats:sup>\u22127<\/jats:sup> M) was the most potent competitor. Western blot analysis of the same gel using rabbit anti-human GnRH-II receptor antiserum identified this band as GnRH-II receptor-like antigen.<\/jats:p>","DOI":"10.1530\/eje.1.02005","type":"journal-article","created":{"date-parts":[[2005,9,27]],"date-time":"2005-09-27T16:52:45Z","timestamp":1127839965000},"page":"605-612","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":27,"title":["GnRH-II receptor-like antigenicity in human placenta and in cancers of the human reproductive organs"],"prefix":"10.1093","volume":"153","author":[{"given":"Nicola","family":"Eicke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas R","family":"Gu\u0308nthert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Viereck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doreen","family":"Siebold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Be\u0301he\u0301","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Becker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gu\u0308nter","family":"Emons","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carsten","family":"Gru\u0308ndker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"issue":"1","key":"1_11451951","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1530\/eje.0.1460001","volume":"146","author":"Grundker","year":"2002","journal-title":"European Journal of Endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0804-4643","issn-type":"print"},{"key":"2_24289040","first-page":"5439","volume":"54","year":"1993","journal-title":"Cancer Research","ISSN":"https:\/\/id.crossref.org\/issn\/0008-5472","issn-type":"print"},{"issue":"6","key":"3_14542687","doi-asserted-by":"publisher","first-page":"1458","DOI":"10.1210\/jc.77.6.1458","volume":"77","author":"Emons","year":"1993","journal-title":"Journal of Clinical Endocrinology &amp; 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