{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:13:33Z","timestamp":1787022813374,"version":"build-2736575974"},"reference-count":52,"publisher":"Bioscientifica","issue":"1","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["eo.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,2,2]]},"abstract":"<jats:p>\n                    Curcumin has been ascribed with countless therapeutic effects, but its impact on testicular function has been scarcely researched. Leydig cells comprise the androgen-secreting population of the testis and may give rise to Leydig cell tumours (LCTs). Due to their steroid-secreting nature, LCTs entail endocrine, reproductive, and psychological disorders. Approximately 10% are malignant and do not respond to chemotherapy and radiotherapy. The aim of this study was to assess curcumin\u2019s impact on Leydig cells\u2019 functions and its potential effect on LCT growth.\n                    <jats:italic>In vitro<\/jats:italic>\n                    assays on MA-10 Leydig cells showed that curcumin (20\u201380 \u00b5mol\/L) stimulates acute steroidogenesis, both in the presence and absence of db-cAMP. This effect is accompanied by an increase in StAR expression. Regarding curcumin\u2019s\n                    <jats:italic>in vitro<\/jats:italic>\n                    cytostatic capacity, we show that 40\u201380 \u00b5mol\/L curcumin reduces MA-10 Leydig cells\u2019 proliferative capacity, which could be explained by the arrest in G2\/M and the reduced viability due to the activation of the apoptotic pathway. Finally, CB6F1 mice were inoculated with MA-10 cells to generate ectopic LCT in both flanks. They received i.p. injections of 20 mg\/kg curcumin or vehicle every other day for 15 days. We unveiled curcumin\u2019s capacity to inhibit LCT growth as evidenced by reduced tumour volume, weight, and area under the growth curves. No detrimental effects on general health parameters or testicular integrity were observed. These results provide novel evidence of curcumin\u2019s effects on the endocrine cell population of the testis and propose this natural compound as a therapeutic agent for LCT.\n                  <\/jats:p>","DOI":"10.1530\/eo-22-0075","type":"journal-article","created":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T06:37:15Z","timestamp":1675319835000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-15","source":"Crossref","is-referenced-by-count":2,"title":["Curcumin effects on Leydig cell functions and potential therapeutic uses"],"prefix":"10.1530","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7874-4101","authenticated-orcid":true,"given":"Trinidad","family":"Raices","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Endocrinology and Signal Transduction, Instituto de Biolog\u00eda y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina"},{"name":"Centro de Investigaciones Endocrinol\u00f3gicas \u2019Dr. C\u00e9sar Bergad\u00e1\u2019 (CEDIE-CONICET-FEI), Divisi\u00f3n de Endocrinolog\u00eda, Hospital de Ni\u00f1os Ricardo Guti\u00e9rrez, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4878-3211","authenticated-orcid":true,"given":"Mar\u00eda","family":"Luisa Varela","sequence":"additional","affiliation":[{"name":"Departments of Neurosurgery, and Cell and Developmental Biology, University of Michigan, School of Medicine, Ann Arbor, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2288-6012","authenticated-orcid":true,"given":"Adriana Mar\u00eda Bel\u00e9n","family":"Abiuso","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Endocrinology and Signal Transduction, Instituto de Biolog\u00eda y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elba N","family":"Pereyra","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Endocrinology and Signal Transduction, Instituto de Biolog\u00eda y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8236-8321","authenticated-orcid":true,"given":"Carolina","family":"Mondillo","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Endocrinology and Signal Transduction, Instituto de Biolog\u00eda y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar P","family":"Pignataro","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Endocrinology and Signal Transduction, Instituto de Biolog\u00eda y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Fernanda","family":"Riera","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Endocrinol\u00f3gicas \u2019Dr. C\u00e9sar Bergad\u00e1\u2019 (CEDIE-CONICET-FEI), Divisi\u00f3n de Endocrinolog\u00eda, Hospital de Ni\u00f1os Ricardo Guti\u00e9rrez, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"bib1","series-title":"Journal of Steroid Biochemistry and Molecular Biology","first-page":"49","article-title":"Combined administration of curcumin and gallic acid inhibits gallic acid-induced suppression of steroidogenesis, sperm output, antioxidant defenses and inflammatory responsive genes","volume":"143","author":"Abarikwu","year":"2014","unstructured":"Abarikwu SOAkiri OFDurojaiye MAAlabi AF2014Combined administration of curcumin and gallic acid inhibits gallic acid-induced suppression of steroidogenesis, sperm output, antioxidant defenses and inflammatory responsive genes. Journal of Steroid Biochemistry and Molecular Biology14349\u201360. (https:\/\/doi.org\/10.1016\/j.jsbmb.2014.02.008)"},{"key":"bib2","series-title":"General and Comparative Endocrinology","first-page":"45","article-title":"Resveratrol and curcumin ameliorate di-(2-ethylhexyl) phthalate induced testicular injury in rats","volume":"225","author":"Abd El-Fattah","year":"2016","unstructured":"Abd El-Fattah AAFahim ATSadik NAHAli BM2016Resveratrol and curcumin ameliorate di-(2-ethylhexyl) phthalate induced testicular injury in rats. General and Comparative Endocrinology22545\u201354. (https:\/\/doi.org\/10.1016\/j.ygcen.2015.09.006)"},{"key":"bib3","series-title":"Reproductive Sciences (Thousand Oaks, Calif.)","first-page":"371","article-title":"Curcumin-loaded iron particle improvement of spermatogenesis in azoospermic mouse induced by long-term scrotal hyperthermia","volume":"28","author":"Afshar","year":"2021","unstructured":"Afshar AAliaghaei ANazarian HAbbaszadeh HANaserzadeh PFathabadi FFAbdi SRaee PAghajanpour FNorouzian M2021Curcumin-loaded iron particle improvement of spermatogenesis in azoospermic mouse induced by long-term scrotal hyperthermia. Reproductive Sciences (Thousand Oaks, Calif.)28371\u2013380. (https:\/\/doi.org\/10.1007\/s43032-020-00288-2)"},{"key":"bib4","series-title":"Nutrition Research","first-page":"67","article-title":"Curcumin induces apoptosis and cell cycle arrest via the activation of reactive oxygen species-independent mitochondrial apoptotic pathway in Smad4 and p53 mutated colon adenocarcinoma HT29 cells","volume":"51","author":"Agarwal","year":"2018","unstructured":"Agarwal AKasinathan AGanesan RBalasubramanian ABhaskaran JSuresh SSrinivasan RAravind KBSivalingam N2018Curcumin induces apoptosis and cell cycle arrest via the activation of reactive oxygen species-independent mitochondrial apoptotic pathway in Smad4 and p53 mutated colon adenocarcinoma HT29 cells. Nutrition Research5167\u201381. (https:\/\/doi.org\/10.1016\/j.nutres.2017.12.011)"},{"key":"bib5","series-title":"Archives of Pathology and Laboratory Medicine","first-page":"311","article-title":"An in-depth look at leydig cell tumor of the testis","volume":"131","author":"Al-Agha","year":"2007","unstructured":"Al-Agha OMAxiotis CA2007An in-depth look at leydig cell tumor of the testis. Archives of Pathology and Laboratory Medicine131311\u2013317. (https:\/\/doi.org\/10.5858\/2007-131-311-AILALC)"},{"key":"bib6","series-title":"Guidelines on Testicular Cancer","author":"Albers","year":"2017","unstructured":"Albers PAlbrecht WAlgaba FBokemeyer CCohn-Cedermark GFizazi KHorwich ALaguna MNicolai N & Oldenburg J2017Guidelines on Testicular Cancer. Arnhem, The Netherlands: European Association of Urology. (available at: https:\/\/uroweb.org\/guidelines\/testicular-cancer\/chapter\/introduction)"},{"key":"bib7","series-title":"Journal of Biological Chemistry","first-page":"42971","article-title":"Involvement of the interaction between p21 and proliferating cell nuclear antigen for the maintenance of G2\/M arrest after DNA damage","volume":"276","author":"Ando","year":"2001","unstructured":"Ando TKawabe TOhara HDucommun BItoh MOkamoto T2001Involvement of the interaction between p21 and proliferating cell nuclear antigen for the maintenance of G2\/M arrest after DNA damage. Journal of Biological Chemistry27642971\u201342977. (https:\/\/doi.org\/10.1074\/jbc.M106460200)"},{"key":"bib8","series-title":"Current Molecular Medicine","first-page":"116","article-title":"Curcumin activates the Nrf2 pathway and induces cellular protection against oxidative injury","volume":"20","author":"Ashrafizadeh","year":"2020","unstructured":"Ashrafizadeh MAhmadi ZMohammadinejad RFarkhondeh TSamarghandian S2020Curcumin activates the Nrf2 pathway and induces cellular protection against oxidative injury. Current Molecular Medicine20116\u2013133. (https:\/\/doi.org\/10.2174\/1566524019666191016150757)"},{"key":"bib9","series-title":"Scientific Reports","article-title":"QuPath: open source software for digital pathology image analysis","volume":"7","author":"Bankhead","year":"2017","unstructured":"Bankhead PLoughrey MBFern\u00e1ndez JADombrowski YMcArt DGDunne PDMcQuaid SGray RTMurray LJColeman HG2017QuPath: open source software for digital pathology image analysis. Scientific Reports7 16878. (https:\/\/doi.org\/10.1038\/s41598-017-17204-5)"},{"key":"bib10","series-title":"International Journal of Molecular Sciences","article-title":"Effects of curcumin and its different formulations in preclinical and clinical studies of peripheral neuropathic and postoperative pain: A comprehensive review","volume":"22","author":"Basu","year":"2021","unstructured":"Basu PMaier C & Basu A2021Effects of curcumin and its different formulations in preclinical and clinical studies of peripheral neuropathic and postoperative pain: A comprehensive review. International Journal of Molecular Sciences224666. (https:\/\/doi.org\/10.3390\/ijms22094666)"},{"key":"bib11","series-title":"Analytical Biochemistry","first-page":"248","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","unstructured":"Bradford MM1976A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry72248\u2013254. (https:\/\/doi.org\/10.1006\/abio.1976.9999)"},{"key":"bib12","series-title":"Molecular Biology of the Cell","first-page":"3965","article-title":"p21-Mediated nuclear retention of cyclin B1-Cdk1 in response to genotoxic stress","volume":"15","author":"Charrier-Savournin","year":"2004","unstructured":"Charrier-Savournin FBCh\u00e2teau MTGire VSedivy JPiette JDulic V2004p21-Mediated nuclear retention of cyclin B1-Cdk1 in response to genotoxic stress. Molecular Biology of the Cell153965\u20133976. (https:\/\/doi.org\/10.1091\/mbc.e03-12-0871)"},{"key":"bib13","series-title":"Food and Chemical Toxicology","article-title":"Curcumin inhibits zearalenone-induced apoptosis and oxidative stress in Leydig cells via modulation of the PTEN\/Nrf2\/Bip signaling pathway","volume":"141","author":"Chen","year":"2020","unstructured":"Chen SYang SWang MChen JHuang SWei ZCheng ZWang HLong MLi P2020Curcumin inhibits zearalenone-induced apoptosis and oxidative stress in Leydig cells via modulation of the PTEN\/Nrf2\/Bip signaling pathway. Food and Chemical Toxicology141 111385. (https:\/\/doi.org\/10.1016\/j.fct.2020.111385)"},{"key":"bib14","series-title":"Reproductive Biology and Endocrinology : RB&E","article-title":"Curcumin protects against palmitic acid-induced apoptosis via the inhibition of endoplasmic reticulum stress in testicular Leydig cells","volume":"17","author":"Chen","year":"2019","unstructured":"Chen ZWen DWang FWang CYang L2019Curcumin protects against palmitic acid-induced apoptosis via the inhibition of endoplasmic reticulum stress in testicular Leydig cells. Reproductive Biology and Endocrinology : RB&E17 71. (https:\/\/doi.org\/10.1186\/s12958-019-0517-4)"},{"key":"bib15","series-title":"Nutrients","article-title":"Dietary curcumin: correlation between bioavailability and health potential","volume":"11","author":"Dei Cas","year":"2019","unstructured":"Dei Cas M & Ghidoni R2019Dietary curcumin: correlation between bioavailability and health potential. Nutrients112147. (https:\/\/doi.org\/10.3390\/nu11092147)"},{"key":"bib16","series-title":"Journal of Reproductive Immunology","first-page":"3","article-title":"Testicular macrophage modulation of Leydig cell steroidogenesis","volume":"57","author":"Hales","year":"2002","unstructured":"Hales DB2002Testicular macrophage modulation of Leydig cell steroidogenesis. Journal of Reproductive Immunology573\u201318. (https:\/\/doi.org\/10.1016\/s0165-0378(0200020-7)"},{"key":"bib17","series-title":"Molecules","article-title":"Curcumin nicotinate selectively induces cancer cell apoptosis and cycle arrest through a P53-mediated mechanism","volume":"24","author":"He","year":"2019","unstructured":"He YCHe LKhoshaba RLu FGCai CZhou FLLiao DF & Cao D2019Curcumin nicotinate selectively induces cancer cell apoptosis and cycle arrest through a P53-mediated mechanism. Molecules244179. (https:\/\/doi.org\/10.3390\/molecules24224179)"},{"key":"bib18","series-title":"Foods (Basel, Switzerland)","article-title":"Curcumin: a review of its effects on human health","volume":"6","author":"Hewlings","year":"2017","unstructured":"Hewlings SJ & Kalman DS2017Curcumin: a review of its effects on human health. Foods (Basel, Switzerland)692. (https:\/\/doi.org\/10.3390\/foods6100092)"},{"key":"bib19","series-title":"Journal of Urology","first-page":"186","article-title":"Fertility preservation for pediatric patients: current state and future possibilities","volume":"198","author":"Johnson","year":"2017","unstructured":"Johnson EKFinlayson CRowell EEGosiengfiao YPavone MELockart BOrwig KEBrannigan REWoodruff TK2017Fertility preservation for pediatric patients: current state and future possibilities. Journal of Urology198186\u2013194. (https:\/\/doi.org\/10.1016\/j.juro.2016.09.159)"},{"key":"bib20","series-title":"Animal Reproduction Science","first-page":"77","article-title":"Direct effect of curcumin on porcine ovarian cell functions","volume":"182","author":"K\u00e1dasi","year":"2017","unstructured":"K\u00e1dasi AMaruniakov\u00e1 N\u0160tochma\u013eov\u00e1 ABauer MGrossmann RHarrath AHKoles\u00e1rov\u00e1 ASirotkin AV2017Direct effect of curcumin on porcine ovarian cell functions. Animal Reproduction Science18277\u201383. (https:\/\/doi.org\/10.1016\/j.anireprosci.2017.05.001)"},{"key":"bib21","series-title":"Journal of Microencapsulation","first-page":"372","article-title":"Colon targeted curcumin microspheres laden with ascorbic acid for bioavailability enhancement","volume":"35","author":"Karade","year":"2018","unstructured":"Karade PGJadhav NR2018Colon targeted curcumin microspheres laden with ascorbic acid for bioavailability enhancement. Journal of Microencapsulation35372\u2013380. (https:\/\/doi.org\/10.1080\/02652048.2018.1501111)"},{"key":"bib22","series-title":"Acta Histochemica","article-title":"Ameliorating effects of curcumin-loaded superparamagnetic iron oxide nanoparticles (SPIONs) on the mouse testis exposed to the transient hyperthermia: A molecular and stereological study","volume":"122","author":"Khosravi","year":"2020","unstructured":"Khosravi AHasani ARahimi KAliaghaei APirani MAzad NRamezani FTamimi ABehnam PRaoofi A2020Ameliorating effects of curcumin-loaded superparamagnetic iron oxide nanoparticles (SPIONs) on the mouse testis exposed to the transient hyperthermia: A molecular and stereological study. Acta Histochemica122 151632. (https:\/\/doi.org\/10.1016\/j.acthis.2020.151632)"},{"key":"bib23","series-title":"Cancer Letters","first-page":"199","article-title":"Curcumin inhibits proliferation, invasion, angiogenesis and metastasis of different cancers through interaction with multiple cell signaling proteins","volume":"269","author":"Kunnumakkara","year":"2008","unstructured":"Kunnumakkara ABAnand PAggarwal BB2008Curcumin inhibits proliferation, invasion, angiogenesis and metastasis of different cancers through interaction with multiple cell signaling proteins. Cancer Letters269199\u2013225. (https:\/\/doi.org\/10.1016\/j.canlet.2008.03.009)"},{"key":"bib24","series-title":"Anticancer Research","first-page":"5039","article-title":"Curcumin induces cell cycle arrest and apoptosis in human osteosarcoma (HOS) cells","volume":"29","author":"Lee","year":"2009","unstructured":"Lee DSLee MKKim JH2009Curcumin induces cell cycle arrest and apoptosis in human osteosarcoma (HOS) cells. Anticancer Research295039\u20135044."},{"key":"bib25","series-title":"Food and Function","first-page":"3770","article-title":"Curcumin dose-dependently improves spermatogenic disorders induced by scrotal heat stress in mice","volume":"6","author":"Lin","year":"2015","unstructured":"Lin CShin DGPark SGChu SBGwon LWLee JGYon JMBaek IJNam SY2015Curcumin dose-dependently improves spermatogenic disorders induced by scrotal heat stress in mice. Food and Function63770\u20133777. (https:\/\/doi.org\/10.1039\/c5fo00726g)"},{"key":"bib26","series-title":"Endocrine Journal","first-page":"833","article-title":"Curcumin downregulates 8-br-cAMP-induced steroidogenesis in mouse Leydig cells by suppressing the expression of Cyp11a1 and StAR independently of the PKA-CREB pathway","volume":"65","author":"Lin","year":"2018","unstructured":"Lin YCChiu CHLiu HCWang JY2018Curcumin downregulates 8-br-cAMP-induced steroidogenesis in mouse Leydig cells by suppressing the expression of Cyp11a1 and StAR independently of the PKA-CREB pathway. Endocrine Journal65833\u2013840. (https:\/\/doi.org\/10.1507\/endocrj.EJ18-0010)"},{"key":"bib27","series-title":"Alternatives to Laboratory Animals","first-page":"591","article-title":"An evaluation of the in vitro cytotoxicities of 50 chemicals by using an electrical current exclusion method versus the neutral red uptake and MTT assays","volume":"33","author":"Lindl","year":"2005","unstructured":"Lindl TLewandowski BSchrey\u00f6gg SSt\u00e4udte A2005An evaluation of the in vitro cytotoxicities of 50 chemicals by using an electrical current exclusion method versus the neutral red uptake and MTT assays. Alternatives to Laboratory Animals33591\u2013601. (https:\/\/doi.org\/10.1177\/026119290503300614)"},{"key":"bib28","series-title":"Excli Journal","first-page":"583","article-title":"Using curcumin to prevent structural impairments of testicles in rats induced by sodium metabisulfite","volume":"16","author":"Mahmoudi","year":"2017","unstructured":"Mahmoudi RHonarmand ZKarbalay-Doust SJafari-Barmak MNikseresht MNoorafshan A2017Using curcumin to prevent structural impairments of testicles in rats induced by sodium metabisulfite. Excli Journal16583\u2013592. (https:\/\/doi.org\/10.17179\/excli2017-143)"},{"key":"bib29","series-title":"Cell Cycle","first-page":"2733","article-title":"Nuclear translocation of cyclin B1 marks the restriction point for terminal cell cycle exit in G2 phase","volume":"13","author":"M\u00fcllers","year":"2014","unstructured":"M\u00fcllers ECascales HSJaiswal HSaurin ATLindqvist A2014Nuclear translocation of cyclin B1 marks the restriction point for terminal cell cycle exit in G2 phase. Cell Cycle132733\u20132743. (https:\/\/doi.org\/10.4161\/15384101.2015.945831)"},{"key":"bib30","series-title":"PDQ\u00ae Integrative","article-title":"PDQ curcumin (curcuma, turmeric) and cancer","year":"2022","unstructured":"National Cancer Institute2022PDQ curcumin (curcuma, turmeric) and cancer. In PDQ\u00ae Integrative. Bethesda,MD: Alternative, and Complementary Therapies Editorial Board."},{"key":"bib31","series-title":"Journal of Medicinal Chemistry","first-page":"1620","article-title":"The essential medicinal chemistry of curcumin","volume":"60","author":"Nelson","year":"2017","unstructured":"Nelson KMDahlin JLBisson JGraham JPauli GFWalters MA2017The essential medicinal chemistry of curcumin. Journal of Medicinal Chemistry601620\u20131637. (https:\/\/doi.org\/10.1021\/acs.jmedchem.6b00975)"},{"key":"bib32","series-title":"Experimental and Toxicologic Pathology","first-page":"627","article-title":"Ameliorative effects of curcumin on the structural parameters of seminiferous tubules and Leydig cells in metronidazole-treated mice: a stereological approach","volume":"63","author":"Noorafshan","year":"2011","unstructured":"Noorafshan AKarbalay-Doust SValizadeh AAliabadi E2011Ameliorative effects of curcumin on the structural parameters of seminiferous tubules and Leydig cells in metronidazole-treated mice: a stereological approach. Experimental and Toxicologic Pathology63627\u2013633. (https:\/\/doi.org\/10.1016\/j.etp.2010.05.004)"},{"key":"bib33","series-title":"Critical Reviews in Food Science and Nutrition","first-page":"887","article-title":"Cellular and molecular mechanisms of curcumin in prevention and treatment of disease","volume":"60","author":"Patel","year":"2020","unstructured":"Patel SSAcharya ARay RSAgrawal RRaghuwanshi RJain P2020Cellular and molecular mechanisms of curcumin in prevention and treatment of disease. Critical Reviews in Food Science and Nutrition60887\u2013939. (https:\/\/doi.org\/10.1080\/10408398.2018.1552244)"},{"key":"bib51","series-title":"Journal of Endocrinology","first-page":"155","article-title":"Effects of heme oxygenase isozymes on Leydig cells steroidogenesis","volume":"203","year":"2009","unstructured":"Piotrkowski BMonz\u00f3n CMPagotto RMReche CGBesio MCymeryng CBPignataro OP2009Effects of heme oxygenase isozymes on Leydig cells steroidogenesis. Journal of Endocrinology203155\u201365. (https:\/\/doi.org\/10.1677\/JOE-09-0061)"},{"key":"bib34","series-title":"Human Reproduction","first-page":"1389","article-title":"Clinical presentation, management and follow-up of 83 patients with Leydig cell tumors of the testis: a prospective case-cohort study","volume":"34","author":"Pozza","year":"2019","unstructured":"Pozza CPofi RTenuta MTarsitano MGSbardella EFattorini GCantisani VLenzi AIsidori AMGianfrilli D2019Clinical presentation, management and follow-up of 83 patients with Leydig cell tumors of the testis: a prospective case-cohort study. Human Reproduction341389\u20131403. (https:\/\/doi.org\/10.1093\/humrep\/dez083)"},{"key":"bib35","series-title":"Molecules (Basel, Switzerland)","first-page":"20091","article-title":"The chemistry of curcumin: from extraction to therapeutic agent","volume":"19","author":"Priyadarsini","year":"2014","unstructured":"Priyadarsini KI2014The chemistry of curcumin: from extraction to therapeutic agent. Molecules (Basel, Switzerland)1920091\u201320112. (https:\/\/doi.org\/10.3390\/molecules191220091)"},{"key":"bib36","series-title":"Journal of Molecular Endocrinology","first-page":"187","article-title":"Heme oxygenase-1 arrests Leydig cells functions and impairs their regulation by histamine","volume":"63","author":"Raices","year":"2019","unstructured":"Raices TVarela MLMonz\u00f3n CMCorrea Torrado MFPagotto RMBesio Moreno MMondillo CPignataro OPPereyra EN2019Heme oxygenase-1 arrests Leydig cells functions and impairs their regulation by histamine. Journal of Molecular Endocrinology63187\u2013197. (https:\/\/doi.org\/10.1530\/JME-19-0063)"},{"key":"bib37","series-title":"Cancer Letters","first-page":"227","article-title":"Inhibition of NF-\u03baB translocation by curcumin analogs induces G0\/G1 arrest and downregulates thymidylate synthase in colorectal cancer","volume":"373","author":"Rajitha","year":"2016","unstructured":"Rajitha BBelalcazar ANagaraju GPShaib WLSnyder JPShoji MPattnaik SAlam AEl-Rayes BF2016Inhibition of NF-\u03baB translocation by curcumin analogs induces G0\/G1 arrest and downregulates thymidylate synthase in colorectal cancer. Cancer Letters373227\u2013233. (https:\/\/doi.org\/10.1016\/j.canlet.2016.01.052)"},{"key":"bib52","series-title":"Reproduction","first-page":"93","article-title":"Metformin counteracts the effects of FSH on rat Sertoli cell proliferation","volume":"156","year":"2018","unstructured":"Rindone GMGorga ARegueira MPellizzari EHCigorraga SBGalardo MNMeroni SB & Riera MF2018Metformin counteracts the effects of FSH on rat Sertoli cell proliferation. Reproduction15693\u2013101. (https:\/\/doi.org\/10.1530\/REP-18-0233)"},{"key":"bib38","series-title":"Gynecological Endocrinology","first-page":"230","article-title":"Sertoli-Leydig cell tumor: a rare ovarian neoplasm. Case report and review of literature","volume":"24","author":"Sachdeva","year":"2008","unstructured":"Sachdeva PArora RDubey CSukhija ADaga MSingh DK2008Sertoli-Leydig cell tumor: a rare ovarian neoplasm. Case report and review of literature. Gynecological Endocrinology24230\u2013234. (https:\/\/doi.org\/10.1080\/09513590801953465)"},{"key":"bib39","series-title":"Biomedicine and Pharmacotherapy\u2009=\u2009Biomedecine and Pharmacotherapie","first-page":"177","article-title":"Curcumin induces G0\/G1 arrest and apoptosis in hormone independent prostate cancer DU-145 cells by down regulating Notch signaling","volume":"84","author":"Sha","year":"2016","unstructured":"Sha JLi JWang WPan LCheng JLi LZhao HLin W2016Curcumin induces G0\/G1 arrest and apoptosis in hormone independent prostate cancer DU-145 cells by down regulating Notch signaling. Biomedicine and Pharmacotherapy\u2009=\u2009Biomedecine and Pharmacotherapie84177\u2013184. (https:\/\/doi.org\/10.1016\/j.biopha.2016.09.037)"},{"key":"bib40","series-title":"Biochemical Pharmacology","first-page":"1167","article-title":"Inhibitory effect of curcumin, a food spice from turmeric, on platelet-activating factor- and arachidonic acid-mediated platelet aggregation through inhibition of thromboxane formation and Ca2+ signaling","volume":"58","author":"Shah","year":"1999","unstructured":"Shah BHNawaz ZPertani SARoomi AMahmood HSaeed SAGilani AH1999Inhibitory effect of curcumin, a food spice from turmeric, on platelet-activating factor- and arachidonic acid-mediated platelet aggregation through inhibition of thromboxane formation and Ca2+ signaling. Biochemical Pharmacology581167\u20131172. (https:\/\/doi.org\/10.1016\/s0006-2952(9900206-3)"},{"key":"bib41","series-title":"Biochemical Pharmacology","first-page":"700","article-title":"Curcumin (diferuloylmethane) inhibits constitutive NF-kappaB activation, induces G1\/S arrest, suppresses proliferation, and induces apoptosis in mantle cell lymphoma","volume":"70","author":"Shishodia","year":"2005","unstructured":"Shishodia SAmin HMLai RAggarwal BB2005Curcumin (diferuloylmethane) inhibits constitutive NF-kappaB activation, induces G1\/S arrest, suppresses proliferation, and induces apoptosis in mantle cell lymphoma. Biochemical Pharmacology70700\u2013713. (https:\/\/doi.org\/10.1016\/j.bcp.2005.04.043)"},{"key":"bib42","series-title":"Planta Medica","first-page":"353","article-title":"Influence of piperine on the pharmacokinetics of curcumin in animals and human volunteers","volume":"64","author":"Shoba","year":"1998","unstructured":"Shoba GJoy DJoseph TMajeed MRajendran RSrinivas PS1998Influence of piperine on the pharmacokinetics of curcumin in animals and human volunteers. Planta Medica64353\u2013356. (https:\/\/doi.org\/10.1055\/s-2006-957450)"},{"key":"bib43","series-title":"Annual Review of Physiology","first-page":"193","article-title":"StAR protein and the regulation of steroid hormone biosynthesis","volume":"63","author":"Stocco","year":"2001","unstructured":"Stocco DM2001StAR protein and the regulation of steroid hormone biosynthesis. Annual Review of Physiology63193\u2013213. (https:\/\/doi.org\/10.1146\/annurev.physiol.63.1.193)"},{"key":"bib44","series-title":"Cancer Epidemiology, Biomarkers and Prevention","first-page":"1411","article-title":"Pharmacokinetics of curcumin conjugate metabolites in healthy human subjects","volume":"17","author":"Vareed","year":"2008","unstructured":"Vareed SKKakarala MRuffin MTCrowell JANormolle DPDjuric ZBrenner DE2008Pharmacokinetics of curcumin conjugate metabolites in healthy human subjects. Cancer Epidemiology, Biomarkers and Prevention171411\u20131417. (https:\/\/doi.org\/10.1158\/1055-9965.EPI-07-2693)"},{"key":"bib45","series-title":"Steroids","article-title":"Effects of dexmedetomidine on the steroidogenesis of rat immature Leydig cells","volume":"149","author":"Wang","year":"2019","unstructured":"Wang YChen YNi CFang YWu KZheng WLi XLin HFan LGe RS2019Effects of dexmedetomidine on the steroidogenesis of rat immature Leydig cells. Steroids149 108423. (https:\/\/doi.org\/10.1016\/j.steroids.2019.05.015)"},{"key":"bib46","series-title":"Environmental Science and Pollution Research International","first-page":"34575","article-title":"Protective role of curcumin in cadmium-induced testicular injury in mice by attenuating oxidative stress via Nrf2\/ARE pathway","volume":"26","author":"Yang","year":"2019","unstructured":"Yang SHHe JBYu LHLi LLong MLiu MDLi P2019Protective role of curcumin in cadmium-induced testicular injury in mice by attenuating oxidative stress via Nrf2\/ARE pathway. Environmental Science and Pollution Research International2634575\u201334583. (https:\/\/doi.org\/10.1007\/s11356-019-06587-9)"},{"key":"bib47","series-title":"Journal of Biochemical and Molecular Toxicology","article-title":"Therapeutic effect of curcumin on acrylamide-induced apoptosis mediated by MAPK signaling pathway in Leydig cells","volume":"33","author":"Yildizbayrak","year":"2019","unstructured":"Yildizbayrak NErkan M2019Therapeutic effect of curcumin on acrylamide-induced apoptosis mediated by MAPK signaling pathway in Leydig cells. Journal of Biochemical and Molecular Toxicology33 e22326. (https:\/\/doi.org\/10.1002\/jbt.22326)"},{"key":"bib48","series-title":"Toxicology in Vitro: an International Journal Published in Association with BIBRA","first-page":"950","article-title":"Mono-(2-ethylhexyl) phthalate affects the steroidogenesis in rat Leydig cells through provoking ROS perturbation","volume":"26","author":"Zhao","year":"2012","unstructured":"Zhao YAo HChen LSottas CMGe RSLi LZhang Y2012Mono-(2-ethylhexyl) phthalate affects the steroidogenesis in rat Leydig cells through provoking ROS perturbation. Toxicology in Vitro: an International Journal Published in Association with BIBRA26950\u2013955. (https:\/\/doi.org\/10.1016\/j.tiv.2012.04.003)"},{"key":"bib49","series-title":"Oncology Reports","article-title":"Curcumin promotes cell cycle arrest and apoptosis of acute myeloid leukemia cells by inactivating AKT","volume":"45","author":"Zhou","year":"2021","unstructured":"Zhou HNing YZeng GZhou C & Ding X2021Curcumin promotes cell cycle arrest and apoptosis of acute myeloid leukemia cells by inactivating AKT. Oncology Reports4511. (https:\/\/doi.org\/10.3892\/or.2021.7962)"},{"key":"bib50","series-title":"Evidence-Based Complementary and Alternative Medicine","article-title":"Curcumin induces autophagy, apoptosis, and cell cycle arrest in human pancreatic cancer cells","volume":"2017","author":"Zhu","year":"2017","unstructured":"Zhu YBu S2017Curcumin induces autophagy, apoptosis, and cell cycle arrest in human pancreatic cancer cells. Evidence-Based Complementary and Alternative Medicine2017 5787218. 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