{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:19:38Z","timestamp":1784557178371,"version":"3.55.0"},"reference-count":33,"publisher":"Bioscientifica","issue":"6","content-domain":{"domain":["erc.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:p>\n                    The\n                    <jats:italic>BRAF<\/jats:italic>\n                    V600E mutation is the most common genetic alteration in thyroid cancer. However, its clinicopathological significance and clonal mutation frequency remain unclear. To clarify the inconsistent results, we investigated the association between the allelic frequency of\n                    <jats:italic>BRAF<\/jats:italic>\n                    V600E and the clinicopathological features of classic papillary thyroid carcinoma (PTC). Tumour tissues from two independent sets of patients with classic PTC were manually microdissected and analysed for the presence or absence of the\n                    <jats:italic>BRAF<\/jats:italic>\n                    mutation and the mutant allelic frequency using quantitative pyrosequencing. For external validation, the Cancer Genome Atlas (TCGA) data were analysed. The\n                    <jats:italic>BRAF<\/jats:italic>\n                    V600E mutation was found in 264 (82.2%) out of 321 classic PTCs in the training set. The presence of\n                    <jats:italic>BRAF<\/jats:italic>\n                    V600E was only associated with extrathyroidal extension and the absence of thyroiditis. In\n                    <jats:italic>BRAF<\/jats:italic>\n                    V600E-positive tumours, the mutant allelic frequency varied from 8 to 41% of the total\n                    <jats:italic>BRAF<\/jats:italic>\n                    alleles (median, 20%) and directly correlated with tumour size and the number of metastatic lymph nodes. Lymph node metastases were more frequent in PTCs with a high (\u226520%) abundance of mutant alleles than in those with a low abundance of mutant alleles (\n                    <jats:italic>P<\/jats:italic>\n                    =0.010). These results were reinforced by validation dataset (\n                    <jats:italic>n<\/jats:italic>\n                    =348) analysis but were not reproduced in the TCGA dataset. In a population with prevalent\n                    <jats:italic>BRAF<\/jats:italic>\n                    mutations, quantitative analysis of the\n                    <jats:italic>BRAF<\/jats:italic>\n                    mutation could provide additional information regarding tumour behaviour, which is not reflected by qualitative analysis. Nonetheless, prospective studies are needed before the mutated allele percentage can be considered as a prognostic factor.\n                  <\/jats:p>","DOI":"10.1530\/erc-14-0147","type":"journal-article","created":{"date-parts":[[2014,9,29]],"date-time":"2014-09-29T22:28:17Z","timestamp":1412029697000},"page":"891-902","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":28,"title":["Quantification of BRAF V600E alleles predicts papillary thyroid cancer progression"],"prefix":"10.1530","volume":"21","author":[{"given":"Min-Hee","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ja Seong","family":"Bae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Jun","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyoungnam","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"So Ra","family":"Jeon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyeong Sin","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chan Kwon","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"1_48950271","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/1755-8794-6-40","volume":"6","year":"2013","journal-title":"BMC MEDICAL GENOMICS"},{"key":"2_48950272","doi-asserted-by":"crossref","first-page":"E1530","DOI":"10.1210\/jc.2013-4389","volume":"99","year":"2014","journal-title":"Journal of Clinical Endocrinology &amp; 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