{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T03:59:41Z","timestamp":1790049581705,"version":"4.0.1"},"reference-count":46,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["erc.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:p>\n                    Adrenocortical carcinoma (ACC) is an aggressive malignancy with high rates of recurrence following surgical resection. Long noncoding RNAs (lncRNAs) play an important role in cancer development. Pathogenesis of adrenal tumours have been characterised by mRNA, microRNA and methylation expression signatures, but it is unknown if this extends to lncRNAs. This study describes lncRNA expression signatures in ACC, adrenal cortical adenoma (ACA) and normal adrenal cortex (NAC) and presents lncRNAs associated with ACC recurrence to identify novel prognostic and therapeutic targets. RNA was extracted from freshly frozen tissue with confirmation of diagnosis by histopathology. Focused lncRNA and mRNA transcriptome analysis was performed using the ArrayStar Human LncRNA V3.0 microarray. Differentially expressed lncRNAs were validated using quantitative reverse transcriptase-PCR and correlated with clinical outcomes. Microarray of 21 samples (ten ACCs, five ACAs and six NACs) showed distinct patterns of lncRNA expression between each group. A total of 956 lncRNAs were differentially expressed between ACC and NAC, including known carcinogenesis-related lncRNAs such as\n                    <jats:italic>H19<\/jats:italic>\n                    ,\n                    <jats:italic>GAS5<\/jats:italic>\n                    ,\n                    <jats:italic>MALAT1<\/jats:italic>\n                    and\n                    <jats:italic>PRINS<\/jats:italic>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    \u22640.05); 85 lncRNAs were differentially expressed between ACC and ACA (\n                    <jats:italic>P<\/jats:italic>\n                    \u22640.05). Hierarchical clustering and heat mapping showed ACC samples correctly grouped compared with NAC and ACA. Sixty-six differentially expressed lncRNAs were found to be associated with ACC recurrence (\n                    <jats:italic>P<\/jats:italic>\n                    \u22640.05), one of which,\n                    <jats:italic>PRINS<\/jats:italic>\n                    , was validated in a group of 20 ACCs and also found to be associated with metastatic disease on presentation. The pathogenesis of adrenal tumours extends to lncRNA dysregulation and low expression of the lncRNA\n                    <jats:italic>PRINS<\/jats:italic>\n                    is associated with ACC recurrence.\n                  <\/jats:p>","DOI":"10.1530\/erc-14-0457","type":"journal-article","created":{"date-parts":[[2015,1,17]],"date-time":"2015-01-17T08:04:43Z","timestamp":1421481883000},"page":"99-109","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":52,"title":["Long noncoding RNA profiles of adrenocortical cancer can be used to predict recurrence"],"prefix":"10.1530","volume":"22","author":[{"given":"A R","family":"Glover","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J T","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J C","family":"Ip","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J C","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B G","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A J","family":"Gill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P S H","family":"Soon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S B","family":"Sidhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"issue":"7","key":"1_22058472","doi-asserted-by":"publisher","first-page":"2650","DOI":"10.1210\/jc.2005-2730","volume":"91","year":"2006","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"https:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"1","key":"2_41178433","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/j.mce.2011.09.044","volume":"351","author":"Assie","year":"2012","journal-title":"Molecular and cellular endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0303-7207","issn-type":"print"},{"key":"3_48277351","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1038\/ng.2953","volume":"46","year":"2014","journal-title":"Nature genetics","ISSN":"https:\/\/id.crossref.org\/issn\/1061-4036","issn-type":"print"},{"key":"4_48289340","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.cell.2014.03.008","volume":"157","year":"2014","journal-title":"Cell","ISSN":"https:\/\/id.crossref.org\/issn\/0092-8674","issn-type":"print"},{"key":"5_47390049","doi-asserted-by":"crossref","first-page":"2419","DOI":"10.1038\/bjc.2013.233","volume":"108","year":"2013","journal-title":"British Journal of Cancer","ISSN":"https:\/\/id.crossref.org\/issn\/1532-1827","issn-type":"print"},{"issue":"9","key":"6_43444869","doi-asserted-by":"publisher","first-page":"1775","DOI":"10.1101\/gr.132159.111","volume":"22","author":"Derrien","year":"2012","journal-title":"Genome Research","ISSN":"https:\/\/id.crossref.org\/issn\/1088-9051","issn-type":"print"},{"key":"7_49574366","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1038\/nsmb.2591","volume":"20","year":"2013","journal-title":"NATURE STRUCTURAL MOLECULAR BIOLOGY"},{"issue":"4","key":"8_22637891","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1016\/j.ygeno.2006.05.012","volume":"88","author":"Emtage","year":"2006","journal-title":"Genomics","ISSN":"https:\/\/id.crossref.org\/issn\/0888-7543","issn-type":"print"},{"issue":"3","key":"9_16953476","doi-asserted-by":"publisher","first-page":"1170","DOI":"10.1210\/jc.87.3.1170","volume":"87","author":"Gao","year":"2002","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"https:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"8","key":"10_16873741","doi-asserted-by":"publisher","first-page":"2559","DOI":"10.1210\/jc.82.8.2559","volume":"82","author":"Gicquel","year":"1997","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"https:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"2","key":"11_33393790","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1158\/1078-0432.CCR-08-1067","volume":"15","author":"Giordano","year":"2009","journal-title":"Clinical Cancer Research","ISSN":"https:\/\/id.crossref.org\/issn\/1078-0432","issn-type":"print"},{"key":"12_49574367","first-page":"635","volume":"6","year":"2013","journal-title":"ONCOTARGETS AND THERAPY"},{"issue":"3","key":"13_43241009","doi-asserted-by":"publisher","first-page":"1017","DOI":"10.1016\/j.ajpath.2012.05.026","volume":"181","author":"Heaton","year":"2012","journal-title":"American Journal Of Pathology","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9440","issn-type":"print"},{"issue":"107","key":"14_36509719","doi-asserted-by":"publisher","first-page":"ra8","DOI":"10.1126\/scisignal.2000568","volume":"3","author":"Kino","year":"2010","journal-title":"Science Signaling","ISSN":"https:\/\/id.crossref.org\/issn\/1945-0877","issn-type":"print"},{"issue":"6","key":"15_45941754","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1634\/theoncologist.2012-0461","volume":"18","year":"2013","journal-title":"The Oncologist","ISSN":"https:\/\/id.crossref.org\/issn\/1083-7159","issn-type":"print"},{"key":"16_48277346","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.mce.2013.10.028","volume":"386","year":"2014","journal-title":"Molecular and cellular endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0303-7207","issn-type":"print"},{"key":"17_49574368","doi-asserted-by":"crossref","first-page":"p594","DOI":"10.1016\/j.tcb.2014.06.003","volume":"24","year":"2014","journal-title":"Trends in cell biology","ISSN":"https:\/\/id.crossref.org\/issn\/0962-8924","issn-type":"print"},{"key":"18_35355429","first-page":"851","volume":"26","year":"2006","journal-title":"Oncogene","ISSN":"https:\/\/id.crossref.org\/issn\/0950-9232","issn-type":"print"},{"key":"19_49574369","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1093\/carcin\/bgn232","volume":"30","year":"2008","journal-title":"Carcinogenesis","ISSN":"https:\/\/id.crossref.org\/issn\/0143-3334","issn-type":"print"},{"issue":"19","key":"20_35555727","doi-asserted-by":"publisher","first-page":"7577","DOI":"10.1158\/0008-5472.CAN-09-0773","volume":"69","year":"2009","journal-title":"Cancer Research","ISSN":"https:\/\/id.crossref.org\/issn\/0008-5472","issn-type":"print"},{"key":"21_49574370","doi-asserted-by":"crossref","first-page":"56e","DOI":"10.1038\/oncsis.2013.21","volume":"2","year":"2013","journal-title":"ONCOGENESIS"},{"key":"22_49574371","first-page":"423","volume":"15","year":"2014","journal-title":"Nature reviews. Cancer","ISSN":"https:\/\/id.crossref.org\/issn\/1474-175X","issn-type":"print"},{"issue":"1","key":"23_38231140","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1016\/j.cell.2010.09.001","volume":"143","author":"Orom","year":"2010","journal-title":"Cell","ISSN":"https:\/\/id.crossref.org\/issn\/0092-8674","issn-type":"print"},{"key":"24_49574372","doi-asserted-by":"crossref","first-page":"3541","DOI":"10.1245\/s10434-014-3768-5","volume":"21","year":"2014","journal-title":"Annals of Surgical Oncology","ISSN":"https:\/\/id.crossref.org\/issn\/1068-9265","issn-type":"print"},{"key":"25_49574373","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s10549-014-2974-y","volume":"145","year":"2014","journal-title":"Breast cancer research and treatment","ISSN":"https:\/\/id.crossref.org\/issn\/0167-6806","issn-type":"print"},{"issue":"5","key":"26_40948031","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1158\/2159-8290.CD-11-0209","volume":"1","author":"Prensner","year":"2011","journal-title":"Cancer Discovery","ISSN":"https:\/\/id.crossref.org\/issn\/2159-8274","issn-type":"print"},{"issue":"34","key":"27_15581905","doi-asserted-by":"crossref","first-page":"21650","DOI":"10.1016\/S0021-9258(17)31855-0","volume":"269","author":"Probst","year":"1994","journal-title":"Journal of Biological Chemistry","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"issue":"5","key":"28_42662131","doi-asserted-by":"publisher","first-page":"e37225","DOI":"10.1371\/journal.pone.0037225","volume":"7","author":"Qian","year":"2012","ISSN":"https:\/\/id.crossref.org\/issn\/1932-6203","issn-type":"print"},{"issue":"21","key":"29_38331912","doi-asserted-by":"publisher","first-page":"8276","DOI":"10.1158\/0008-5472.CAN-10-2014","volume":"70","year":"2010","journal-title":"Cancer Research","ISSN":"https:\/\/id.crossref.org\/issn\/0008-5472","issn-type":"print"},{"issue":"6","key":"30_42316487","doi-asserted-by":"publisher","first-page":"E1004","DOI":"10.1210\/jc.2011-3298","volume":"97","year":"2012","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"https:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"7","key":"31_33362937","doi-asserted-by":"publisher","first-page":"1108","DOI":"10.1200\/JCO.2008.18.5678","volume":"27","year":"2009","journal-title":"Journal of Clinical Oncology","ISSN":"https:\/\/id.crossref.org\/issn\/0732-183X","issn-type":"print"},{"issue":"11","key":"32_31846267","doi-asserted-by":"publisher","first-page":"2106","DOI":"10.1093\/carcin\/bgn206","volume":"29","year":"2008","journal-title":"Carcinogenesis","ISSN":"https:\/\/id.crossref.org\/issn\/0143-3334","issn-type":"print"},{"issue":"1","key":"33_47702103","doi-asserted-by":"publisher","first-page":"R1","DOI":"10.1530\/EJE-14-0273","volume":"171","year":"2014","journal-title":"European Journal of Endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0804-4643","issn-type":"print"},{"key":"34_49574374","first-page":"431","volume":"27","year":"2011","journal-title":"Journal of Gerontology","ISSN":"https:\/\/id.crossref.org\/issn\/0022-1422","issn-type":"print"},{"issue":"25","key":"35_19575084","doi-asserted-by":"publisher","first-page":"24159","DOI":"10.1074\/jbc.M501704200","volume":"280","year":"2005","journal-title":"Journal of Biological Chemistry","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"issue":"24","key":"36_36122417","doi-asserted-by":"publisher","first-page":"7684","DOI":"10.1158\/1078-0432.CCR-09-1587","volume":"15","year":"2009","journal-title":"Clinical Cancer Research","ISSN":"https:\/\/id.crossref.org\/issn\/1078-0432","issn-type":"print"},{"issue":"1","key":"37_36473772","doi-asserted-by":"publisher","first-page":"e8658","DOI":"10.1371\/journal.pone.0008658","volume":"5","author":"Spigoni","year":"2010","ISSN":"https:\/\/id.crossref.org\/issn\/1932-6203","issn-type":"print"},{"issue":"43","key":"38_41770585","doi-asserted-by":"publisher","first-page":"4577","DOI":"10.1038\/onc.2011.621","volume":"31","author":"Spizzo","year":"2012","journal-title":"Oncogene","ISSN":"https:\/\/id.crossref.org\/issn\/0950-9232","issn-type":"print"},{"issue":"3","key":"39_37078999","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1111\/j.1600-0625.2010.01066.x","volume":"19","author":"Szegedi","year":"2010","journal-title":"Experimental dermatology","ISSN":"https:\/\/id.crossref.org\/issn\/0906-6705","issn-type":"print"},{"issue":"11","key":"40_35727874","doi-asserted-by":"publisher","first-page":"4591","DOI":"10.1210\/jc.2009-0546","volume":"94","year":"2009","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"https:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"key":"41_47247679","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1038\/nrd4018","volume":"12","year":"2013","journal-title":"Nature reviews. Drug discovery","ISSN":"https:\/\/id.crossref.org\/issn\/1474-1776","issn-type":"print"},{"issue":"3","key":"42_13175981","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1097\/00000478-198403000-00001","volume":"8","author":"Weiss","year":"1984","journal-title":"The American journal of surgical pathology","ISSN":"https:\/\/id.crossref.org\/issn\/0147-5185","issn-type":"print"},{"issue":"3","key":"43_5158240","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1097\/00000478-198903000-00004","volume":"13","author":"Weiss","year":"1989","journal-title":"The American journal of surgical pathology","ISSN":"https:\/\/id.crossref.org\/issn\/0147-5185","issn-type":"print"},{"issue":"11","key":"44_33464333","doi-asserted-by":"publisher","first-page":"2526","DOI":"10.1182\/blood-2008-06-162164","volume":"113","year":"2009","journal-title":"Blood","ISSN":"https:\/\/id.crossref.org\/issn\/0006-4971","issn-type":"print"},{"key":"45_49574375","doi-asserted-by":"crossref","first-page":"777","DOI":"10.4161\/rna.28828","volume":"11","year":"2014","journal-title":"RNA BIOLOGY"},{"issue":"3","key":"46_42016155","doi-asserted-by":"publisher","first-page":"R45","DOI":"10.1530\/JME-12-0008","volume":"48","year":"2012","journal-title":"Journal of Molecular Endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0952-5041","issn-type":"print"}],"container-title":["Endocrine-Related Cancer"],"original-title":[],"link":[{"URL":"https:\/\/erc.bioscientifica.com\/view\/journals\/erc\/22\/1\/99.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/erc.bioscientifica.com\/downloadpdf\/journals\/erc\/22\/1\/99.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:52:48Z","timestamp":1784555568000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/erc\/article\/22\/1\/99-109\/33770"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2]]},"references-count":46,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/erc-14-0457","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.725325721.793508349","asserted-by":"object"}]},"ISSN":["1351-0088","1479-6821"],"issn-type":[{"value":"1351-0088","type":"print"},{"value":"1479-6821","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2]]}}}