{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T17:43:22Z","timestamp":1790099002545,"version":"4.0.1"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["erc.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,1]]},"abstract":"\n Sorafenib, a small-molecule tyrosine kinase inhibitor with antiangiogenic activity, has been used in liver cancer and kidney cancer treatments. However, clinical trials with sorafenib for breast cancer were stopped in phase III due to limited efficacy. The existence of heterogeneous vasculatures involving tumor cells, such as vessel-like structures formed by vasculogenic mimicry and mosaic vessels, and their resistance to antiangiogenic therapy are thought to be a possible reason for failure of sorafenib therapy. Nevertheless, the features and mechanism of vasculogenesis by tumor cells remain unclear. In the present study, we found that breast cancer stem-like cells (BCSLCs, ALDH1\n +<\/jats:sup>\n cells) were involved in vasculogenic mimicry and mosaic vessel formation in triple-negative breast cancer tissues. Further, only ALDH1\n +<\/jats:sup>\n BCSLCs sorted from MDA-MB-231 could exhibit the tube formation and angiogenesis ability. Sorafenib could inhibit vascularization from endothelial cells rather than that from ALDH1\n +<\/jats:sup>\n cells. \u03b1-SMA was identified as a key molecule in vascular formation of BCSLCs. Mechanistically, HIF-1\u03b1 enhanced the mRNA and protein levels of \u03b1-SMA by binding to the HRE element in the promoter directly and meanwhile increased the BCSLCs population. Interestingly, pigment epithelium-derived factor (PEDF), an endogenous angiogenesis inhibitor, could inhibit both endothelial cell-derived and tumor cell-derived angiogenesis by downregulating HIF-1\u03b1 in breast cancer. Our finding clarified the possible reason for the poor outcome of anti-angiogenesis therapy and PEDF may have the therapeutic potential.\n <\/jats:p>","DOI":"10.1530\/erc-19-0054","type":"journal-article","created":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T09:56:49Z","timestamp":1573120609000},"page":"23-39","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":26,"title":["Stem-like tumor cells involved in heterogeneous vasculogenesis in breast cancer"],"prefix":"10.1530","volume":"27","author":[{"given":"Yuling","family":"Mao","sequence":"first","affiliation":[{"name":"1Program of Molecular Medicine, Affiliated Guangzhou Women and Children\u2019s Medical Center, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"},{"name":"2Department of Reproductive Medicine Center, Key Laboratory for Reproductive Medicine of Guangdong Province, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuqing","family":"Zhu","sequence":"additional","affiliation":[{"name":"1Program of Molecular Medicine, Affiliated Guangzhou Women and Children\u2019s Medical Center, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"},{"name":"3Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijian","family":"Huang","sequence":"additional","affiliation":[{"name":"3Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanghua","family":"Luo","sequence":"additional","affiliation":[{"name":"3Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ti","family":"Zhou","sequence":"additional","affiliation":[{"name":"3Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Li","sequence":"additional","affiliation":[{"name":"2Department of Reproductive Medicine Center, Key Laboratory for Reproductive Medicine of Guangdong Province, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guannan","family":"Wang","sequence":"additional","affiliation":[{"name":"3Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"},{"name":"4Sun Yat-sen University Cancer Center, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhonghan","family":"Yang","sequence":"additional","affiliation":[{"name":"3Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Qi","sequence":"additional","affiliation":[{"name":"1Program of Molecular Medicine, Affiliated Guangzhou Women and Children\u2019s Medical Center, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"},{"name":"3Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Yang","sequence":"additional","affiliation":[{"name":"1Program of Molecular Medicine, Affiliated Guangzhou Women and Children\u2019s Medical Center, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"},{"name":"3Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"},{"name":"5China Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoquan","family":"Gao","sequence":"additional","affiliation":[{"name":"1Program of Molecular Medicine, Affiliated Guangzhou Women and Children\u2019s Medical Center, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"},{"name":"3Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"},{"name":"6Guangdong Engineering & Technology Research Center for Gene Manipulation and Biomacromolecular Products (Sun Yat-sen University), Guangzhou, China"},{"name":"7Guangdong Province Key Laboratory of Brain Function and Disease, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Endocrine-Related Cancer"],"original-title":[],"link":[{"URL":"https:\/\/erc.bioscientifica.com\/view\/journals\/erc\/27\/1\/ERC-19-0054.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/erc.bioscientifica.com\/downloadpdf\/journals\/erc\/27\/1\/ERC-19-0054.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:55:16Z","timestamp":1784555716000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/erc\/article\/27\/1\/23-39\/25334"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/erc-19-0054","relation":{},"ISSN":["1351-0088","1479-6821"],"issn-type":[{"value":"1351-0088","type":"print"},{"value":"1479-6821","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1]]}}}