{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:20:58Z","timestamp":1784557258845,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["erc.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:p>Thyroid carcinoma incidence rates in western societies are among the fastest rising, compared to all malignant tumors over the past two decades. While risk factors such as age and exposure to ionizing radiation are known, early-state carcinogenic processes or pre-lesions are poorly understood or unknown. This study aims at the identification and characterization of early-state radiation-associated neoplastic processes by histologic and transcriptomic analyses of thyroid tissues derived from a mouse model. Comprehensive histological examination of 246 thyroids (164 exposed, 82 non-exposed) was carried out. Proliferative and normal tissues from exposed cases and normal tissue from non-exposed cases were collected by laser-capture microdissection, followed by RNAseq transcriptomic profiling using a low input 3\u2032-library preparation protocol, differential gene expression analysis and functional association by gene set enrichment analysis. Nine exposed samples exhibited proliferative lesions, while none of the non-exposed samples showed histological abnormalities, indicating an association of ionizing radiation exposure with histological abnormalities. Activated immune response signaling and deregulated metabolic processes were observed in irradiated tissue with normal histology compared to normal tissue from non-exposed samples. Proliferative lesions compared to corresponding normal tissues showed enrichment for mainly proliferation-associated gene sets. Consistently, proliferative lesion samples from exposed mice showed elevated proliferation-associated signaling and deregulated metabolic processes compared to normal samples from non-exposed mice. Our findings suggest that a molecular deregulation may be detectable in histologically normal thyroid tissues and in early proliferative lesions in the frame of multi-step progression from irradiated normal tissue to tumorous lesions.<\/jats:p>","DOI":"10.1530\/erc-21-0019","type":"journal-article","created":{"date-parts":[[2021,2,19]],"date-time":"2021-02-19T11:25:50Z","timestamp":1613733950000},"page":"213-224","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-8","source":"Crossref","is-referenced-by-count":1,"title":["Transcriptomic landscape of radiation-induced murine thyroid proliferative lesions"],"prefix":"10.1530","volume":"28","author":[{"given":"Elena","family":"Stauffer","sequence":"first","affiliation":[{"name":"1Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health, Research Unit Radiation Cytogenetics, Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Weber","sequence":"additional","affiliation":[{"name":"1Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health, Research Unit Radiation Cytogenetics, Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Heider","sequence":"additional","affiliation":[{"name":"1Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health, Research Unit Radiation Cytogenetics, Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Dalke","sequence":"additional","affiliation":[{"name":"2Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health, Institute of Metabolism and Cell Death, Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Blutke","sequence":"additional","affiliation":[{"name":"3Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health, Research Unit Analytical Pathology, Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Walch","sequence":"additional","affiliation":[{"name":"3Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health, Research Unit Analytical Pathology, Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald","family":"Burgstaller","sequence":"additional","affiliation":[{"name":"4Helmholtz Zentrum M\u00fcnchen \u2013 Institute of Lung Biology and Disease (ILBD) and Comprehensive Pneumology Center (CPC), Member of the German Center for Lung Research (DZL), Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikko","family":"Brix","sequence":"additional","affiliation":[{"name":"5Department of Radiation Oncology, University Hospital, LMU Munich, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Lauber","sequence":"additional","affiliation":[{"name":"5Department of Radiation Oncology, University Hospital, LMU Munich, Munich, Germany"},{"name":"6Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health GmbH, Clinical Cooperation Group \u2018Personalized Radiotherapy in Head and Neck Cancer\u2019, Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horst","family":"Zitzelsberger","sequence":"additional","affiliation":[{"name":"1Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health, Research Unit Radiation Cytogenetics, Neuherberg, Germany"},{"name":"6Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health GmbH, Clinical Cooperation Group \u2018Personalized Radiotherapy in Head and Neck Cancer\u2019, Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristian","family":"Unger","sequence":"additional","affiliation":[{"name":"1Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health, Research Unit Radiation Cytogenetics, Neuherberg, Germany"},{"name":"5Department of Radiation Oncology, University Hospital, LMU Munich, Munich, Germany"},{"name":"6Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health GmbH, Clinical Cooperation Group \u2018Personalized Radiotherapy in Head and Neck Cancer\u2019, Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Selmansberger","sequence":"additional","affiliation":[{"name":"1Helmholtz Zentrum M\u00fcnchen \u2013 German Research Center for Environmental Health, Research Unit Radiation Cytogenetics, Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Endocrine-Related Cancer"],"original-title":[],"link":[{"URL":"https:\/\/erc.bioscientifica.com\/view\/journals\/erc\/28\/3\/ERC-21-0019.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/erc.bioscientifica.com\/downloadpdf\/journals\/erc\/28\/3\/ERC-21-0019.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:55:57Z","timestamp":1784555757000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/erc\/article\/28\/3\/213-224\/25547"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1530\/erc-21-0019","relation":{},"ISSN":["1351-0088","1479-6821"],"issn-type":[{"value":"1351-0088","type":"print"},{"value":"1479-6821","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3]]}}}