{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:40:32Z","timestamp":1787920832095,"version":"build-2784847793"},"reference-count":35,"publisher":"Bioscientifica","issue":"1","license":[{"start":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T00:00:00Z","timestamp":1772236800000},"content-version":"vor","delay-in-days":493,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The molecular biology of pituitary neuroendocrine tumors (PitNETs) revealed few recurrent mutations and extensive chromosomal alterations, with the latter being the driving force in a subset of these lesions. Addressing the need for an easily applicable diagnostic tool, we conducted a retrospective study of 61 PitNETs operated at a tertiary care center. All cases were subtyped according to the 2022 WHO Classification of Endocrine Tumors. A genome-wide next-generation sequencing panel targeting 1500 single nucleotide polymorphisms (SNPs) was used to classify chromosomal imbalances, loss of heterozygosity, and copy number variations in DNA from formalin-fixed paraffin-embedded tissues. We identified four distinct chromosomal patterns, with varying distribution among different tumor lineages. Forty-two of 61 (69%) PitNETs showed chromosomal alterations. Gonadotroph PitNETs showed mostly quiet genomes. The majority of lactotroph PitNETs (19\/20, 95%) were altered, exhibiting a gained genome and a remarkably low recurrence rate. Nine of ten (90%) corticotroph PitNETs harbored chromosomal alterations, of which two aggressive corticotroph tumors and one metastatic corticotroph PitNET showed massive chromosomal losses, leading to near-haploid\/near-homozygous genomes. The comparison of the molecular profile of primary and recurrent PitNETs of five patients showed no significant accumulation of alterations over time. A simple genome-wide 1500-SNP test can be used in the identification of outspoken aggressive subsets of PitNETs by the occurrence of a near-haploid\/near-homozygous genome. Furthermore, the presence of neoplastic tissue in the resected material can be potentially confirmed for non-gonadotroph PitNETs under suboptimal histological assessment conditions.<\/jats:p>","DOI":"10.1530\/erc-24-0070","type":"journal-article","created":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T13:39:53Z","timestamp":1729690793000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-8","source":"Crossref","is-referenced-by-count":10,"title":["Chromosomal alteration patterns in PitNETs: massive losses in aggressive tumors"],"prefix":"10.1530","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5724-7643","authenticated-orcid":true,"given":"Maaia Margo","family":"Jentus","sequence":"first","affiliation":[{"name":"Department of Pathology, Leiden University Medical Center 1 , ,","place":["Leiden, The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leontine","family":"Bakker","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Endocrinology, Leiden University Medical Center 2 , ,","place":["Leiden, The Netherlands"]},{"name":"Center for Endocrine Tumors Leiden (CETL), Pituitary Center, Leiden University Medical Center 3 , ,","place":["Leiden, The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Verstegen","sequence":"additional","affiliation":[{"name":"Center for Endocrine Tumors Leiden (CETL), Pituitary Center, Leiden University Medical Center 3 , ,","place":["Leiden, The Netherlands"]},{"name":"Department of Neurosurgery, Center for Endocrine Tumors Leiden (CETL), Leiden University Medical Center 4 , ,","place":["Leiden, The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iris","family":"Pelsma","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Endocrinology, Leiden University Medical Center 2 , ,","place":["Leiden, The Netherlands"]},{"name":"Center for Endocrine Tumors Leiden (CETL), Pituitary Center, Leiden University Medical Center 3 , ,","place":["Leiden, The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"van Wezel","sequence":"additional","affiliation":[{"name":"Department of Pathology, Leiden University Medical Center 1 , ,","place":["Leiden, The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dina","family":"Ruano","sequence":"additional","affiliation":[{"name":"Department of Pathology, Leiden University Medical Center 1 , ,","place":["Leiden, The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Kapiteijn","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Leiden University Medical Center 5 , ,","place":["Leiden, The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stijn","family":"Crobach","sequence":"additional","affiliation":[{"name":"Department of Pathology, Leiden University Medical Center 1 , ,","place":["Leiden, The Netherlands"]},{"name":"Center for Endocrine Tumors Leiden (CETL), Pituitary Center, Leiden University Medical Center 3 , ,","place":["Leiden, The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nienke","family":"Biermasz","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Endocrinology, Leiden University Medical Center 2 , ,","place":["Leiden, The Netherlands"]},{"name":"Center for Endocrine Tumors Leiden (CETL), Pituitary Center, Leiden University Medical Center 3 , ,","place":["Leiden, The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Morreau","sequence":"additional","affiliation":[{"name":"Department of Pathology, Leiden University Medical Center 1 , ,","place":["Leiden, The Netherlands"]},{"name":"Center for Endocrine Tumors Leiden (CETL), Pituitary Center, Leiden University Medical Center 3 , ,","place":["Leiden, The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","published-online":{"date-parts":[[2024,10,23]]},"reference":[{"key":"2026070905431660000_bib1","doi-asserted-by":"crossref","first-page":"R14","DOI":"10.1530\/EO-22-0041","article-title":"Pituitary carcinoma: reclassification and implications in the NET schema","volume":"2","author":"Asa","year":"2022","journal-title":"Endocr 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