{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:34:30Z","timestamp":1791074070454,"version":"4.1.0"},"reference-count":40,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:p>\n                    Metallothioneins (MTs) are cytoprotective proteins acting as scavengers of toxic metal ions or reactive oxygen species. MTs are upregulated in follicular thyroid carcinoma and are regarded as a marker of thyroid stress in Graves' disease. However, the mechanism of MT regulation in thyrocytes is still elusive. In other cellular systems, cAMP-, calcium-, or protein kinase C (PKC)-dependent signaling cascades have been shown to induce MT expression. Of note, all of these three pathways are activated following the stimulation of the TSH receptor (TSHR). Thus, we hypothesized that TSH represents a key regulator of MT expression in thyrocytes. In fact, TSHR stimulation induced expression of MT isoform 1X (MT1X) in human follicular carcinoma cells. In these cells, Induction of MT1X expression critically relied on intact G\n                    <jats:sub>q\/11<\/jats:sub>\n                    signaling of the TSHR and was blocked by chelation of intracellular calcium and inhibition of PKC. TSHR-independent stimulation of cAMP formation by treating cells with forskolin also led to an upregulation of MT1X, which was completely dependent on PKA. However, inhibition of PKA did not affect the regulation of MT1X by TSH. As in follicular thyroid carcinoma cells, TSH also induced MT1 protein in primary human thyrocytes, which was PKC dependent as well. In summary, these findings indicate that TSH stimulation induces MT1X expression via G\n                    <jats:sub>q\/11<\/jats:sub>\n                    and PKC, whereas cAMP\u2013PKA signaling does not play a predominant role. To date, little has been known regarding cAMP-independent effects of TSHR signaling. Our findings extend the knowledge about the PKC-mediated functions of the TSHR.\n                  <\/jats:p>","DOI":"10.1530\/jme-12-0200","type":"journal-article","created":{"date-parts":[[2013,4,23]],"date-time":"2013-04-23T23:55:40Z","timestamp":1366761340000},"page":"79-90","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":13,"title":["TSH induces metallothionein 1 in thyrocytes via Gq\/11- and PKC-dependent signaling"],"prefix":"10.1530","volume":"51","author":[{"given":"Christer M","family":"B\u00e4ck","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie","family":"Stohr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva A M","family":"Sch\u00e4fer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heike","family":"Biebermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Boekhoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Breit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Gudermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas R H","family":"B\u00fcch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"issue":"Pt 1","key":"1_21534490","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1042\/BJ20051143","volume":"394","author":"Baird","year":"2006","journal-title":"The Biochemical journal","ISSN":"https:\/\/id.crossref.org\/issn\/0264-6021","issn-type":"print"},{"issue":"15","key":"2_15584254","doi-asserted-by":"publisher","first-page":"3167","DOI":"10.1093\/nar\/22.15.3167","volume":"22","author":"Brugnera","year":"1994","journal-title":"Nucleic Acids Research","ISSN":"https:\/\/id.crossref.org\/issn\/0305-1048","issn-type":"print"},{"issue":"29","key":"3_30986997","doi-asserted-by":"publisher","first-page":"20330","DOI":"10.1074\/jbc.M800211200","volume":"283","year":"2008","journal-title":"Journal of Biological Chemistry","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"issue":"8","key":"4_35479908","doi-asserted-by":"publisher","first-page":"e1000172","DOI":"10.1371\/journal.pbio.1000172","volume":"7","author":"Calebiro","year":"2009","journal-title":"PLoS biology","ISSN":"https:\/\/id.crossref.org\/issn\/1544-9173","issn-type":"print"},{"issue":"1-2","key":"5_17934303","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.mrfmmm.2003.07.013","volume":"533","author":"Cherian","year":"2003","journal-title":"Mutation Research","ISSN":"https:\/\/id.crossref.org\/issn\/0027-5107","issn-type":"print"},{"key":"6_34834489","first-page":"E688","volume":"251","year":"1986","journal-title":"American Journal of Physiology -- Legacy Content","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9513","issn-type":"print"},{"issue":"7","key":"7_37621305","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1089\/thy.2010.1638","volume":"20","author":"Davies","year":"2010","journal-title":"Thyroid : official journal of the American Thyroid Association","ISSN":"https:\/\/id.crossref.org\/issn\/1050-7256","issn-type":"print"},{"issue":"9","key":"8_18297304","doi-asserted-by":"publisher","first-page":"4062","DOI":"10.1210\/en.2004-0501","volume":"145","year":"2004","journal-title":"Endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"3","key":"9_22037459","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.bbamcr.2005.12.001","volume":"1763","author":"Eszlinger","year":"2006","journal-title":"Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology","ISSN":"https:\/\/id.crossref.org\/issn\/0006-3002","issn-type":"print"},{"issue":"5","key":"10_30959030","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1038\/labinvest.2008.17","volume":"88","author":"Ferrario","year":"2008","journal-title":"Laboratory investigation; a journal of technical methods and pathology","ISSN":"https:\/\/id.crossref.org\/issn\/0023-6837","issn-type":"print"},{"issue":"7","key":"11_28455904","doi-asserted-by":"publisher","first-page":"2816","DOI":"10.1210\/jc.2007-0366","volume":"92","year":"2007","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"https:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"12","key":"12_23309831","doi-asserted-by":"crossref","first-page":"2870","DOI":"10.1002\/j.1460-2075.1994.tb06581.x","volume":"13","author":"Heuchel","year":"1994","journal-title":"The EMBO Journal","ISSN":"https:\/\/id.crossref.org\/issn\/0261-4189","issn-type":"print"},{"issue":"6","key":"13_17541036","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1002\/ijc.11006","volume":"104","author":"Huang","year":"2003","journal-title":"International journal of cancer. Journal international du cancer","ISSN":"https:\/\/id.crossref.org\/issn\/0020-7136","issn-type":"print"},{"issue":"9","key":"14_29200869","doi-asserted-by":"publisher","first-page":"2399","DOI":"10.1172\/JCI30380","volume":"117","year":"2007","journal-title":"Journal of Clinical Investigation","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9738","issn-type":"print"},{"issue":"1","key":"15_15737822","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1210\/en.136.1.116","volume":"136","author":"Kimura","year":"1995","journal-title":"Endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"12","key":"16_38895762","first-page":"4945","volume":"30","author":"KROLICKA","year":"2010","journal-title":"Anticancer Research","ISSN":"https:\/\/id.crossref.org\/issn\/0250-7005","issn-type":"print"},{"issue":"45","key":"17_19551200","doi-asserted-by":"publisher","first-page":"41879","DOI":"10.1074\/jbc.M108313200","volume":"276","year":"2001","journal-title":"Journal of Biological Chemistry","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"issue":"1","key":"18_16144487","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1073\/pnas.93.1.116","volume":"93","author":"Laugwitz","year":"1996","journal-title":"PNAS","ISSN":"https:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"1","key":"19_19516295","doi-asserted-by":"publisher","first-page":"765","DOI":"10.1074\/jbc.M307907200","volume":"279","year":"2004","journal-title":"Journal of Biological Chemistry","ISSN":"https:\/\/id.crossref.org\/issn\/0021-9258","issn-type":"print"},{"issue":"13","key":"20_28910025","doi-asserted-by":"publisher","first-page":"2465","DOI":"10.1016\/j.febslet.2007.04.049","volume":"581","author":"Liu","year":"2007","journal-title":"FEBS Letters","ISSN":"https:\/\/id.crossref.org\/issn\/1873-3468","issn-type":"print"},{"issue":"1","key":"21_34561293","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/j.mce.2008.12.017","volume":"302","author":"Liu","year":"2009","journal-title":"Molecular and cellular endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0303-7207","issn-type":"print"},{"issue":"1","key":"22_22827229","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.tox.2006.08.017","volume":"228","author":"Mori","year":"2006","journal-title":"Toxicology","ISSN":"https:\/\/id.crossref.org\/issn\/0300-483X","issn-type":"print"},{"issue":"1","key":"23_14275715","first-page":"177","volume":"129","author":"Nartey","year":"1987","journal-title":"American Journal Of Pathology","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9440","issn-type":"print"},{"issue":"2-3","key":"24_11290724","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/S0300-483X(01)00375-4","volume":"163","author":"Park","year":"2001","journal-title":"Toxicology","ISSN":"https:\/\/id.crossref.org\/issn\/0300-483X","issn-type":"print"},{"issue":"5","key":"25_18807502","doi-asserted-by":"publisher","first-page":"2473","DOI":"10.1210\/en.2004-1588","volume":"146","year":"2005","journal-title":"Endocrinology","ISSN":"https:\/\/id.crossref.org\/issn\/0013-7227","issn-type":"print"},{"issue":"1","key":"26_34544907","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.proghi.2008.10.001","volume":"44","author":"Pedersen","year":"2009","journal-title":"Progress in histochemistry and cytochemistry","ISSN":"https:\/\/id.crossref.org\/issn\/0079-6336","issn-type":"print"},{"issue":"3","key":"27_36412203","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.2353\/ajpath.2010.090682","volume":"176","year":"2010","journal-title":"American Journal Of Pathology","ISSN":"https:\/\/id.crossref.org\/issn\/0002-9440","issn-type":"print"},{"issue":"3-4","key":"28_39357092","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/j.jaut.2011.01.002","volume":"36","author":"Ruiz-Riol","year":"2011","journal-title":"Journal of autoimmunity","ISSN":"https:\/\/id.crossref.org\/issn\/0896-8411","issn-type":"print"},{"issue":"2","key":"29_41206023","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1210\/jc.2011-1429","volume":"97","year":"2012","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"https:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"key":"30_45952157","first-page":"133","volume":"3","year":"2012","journal-title":"FRONTIERS IN ENDOCRINOLOGY"},{"issue":"9","key":"31_31435301","doi-asserted-by":"publisher","first-page":"1577","DOI":"10.1089\/ars.2008.2054","volume":"10","author":"Schweizer","year":"2008","journal-title":"Antioxidants &amp; redox signaling","ISSN":"https:\/\/id.crossref.org\/issn\/1523-0864","issn-type":"print"},{"issue":"2","key":"32_21288251","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1073\/pnas.0507354102","volume":"103","year":"2006","journal-title":"PNAS","ISSN":"https:\/\/id.crossref.org\/issn\/0027-8424","issn-type":"print"},{"issue":"17","key":"33_29336007","doi-asserted-by":"publisher","first-page":"8113","DOI":"10.1158\/0008-5472.CAN-06-4026","volume":"67","author":"van Staveren","year":"2007","journal-title":"Cancer Research","ISSN":"https:\/\/id.crossref.org\/issn\/0008-5472","issn-type":"print"},{"issue":"3","key":"34_21289172","doi-asserted-by":"publisher","first-page":"1099","DOI":"10.1210\/jc.2005-1324","volume":"91","year":"2006","journal-title":"Journal of Clinical Endocrinology &amp; Metabolism","ISSN":"https:\/\/id.crossref.org\/issn\/0021-972X","issn-type":"print"},{"issue":"3","key":"35_10023428","doi-asserted-by":"publisher","first-page":"596","DOI":"10.1210\/edrv-13-3-596","volume":"13","author":"VASSART","year":"1992","journal-title":"Endocrine Reviews","ISSN":"https:\/\/id.crossref.org\/issn\/0163-769X","issn-type":"print"},{"issue":"2","key":"36_6559200","first-page":"407","volume":"46","author":"Viarengo","year":"2000","journal-title":"Cellular and molecular biology","ISSN":"https:\/\/id.crossref.org\/issn\/0145-5680","issn-type":"print"},{"issue":"6","key":"37_41007810","doi-asserted-by":"publisher","first-page":"773","DOI":"10.1530\/ERC-11-0230","volume":"18","year":"2011","journal-title":"Endocrine-Related Cancer","ISSN":"https:\/\/id.crossref.org\/issn\/1351-0088","issn-type":"print"},{"issue":"9","key":"38_40445386","first-page":"2833","volume":"31","author":"WERYNSKA","year":"2011","journal-title":"Anticancer Research","ISSN":"https:\/\/id.crossref.org\/issn\/0250-7005","issn-type":"print"},{"issue":"1","key":"39_41350814","doi-asserted-by":"publisher","first-page":"C7","DOI":"10.1530\/ERC-11-0360","volume":"19","year":"2012","journal-title":"Endocrine-Related Cancer","ISSN":"https:\/\/id.crossref.org\/issn\/1351-0088","issn-type":"print"},{"issue":"4","key":"40_30200737","doi-asserted-by":"crossref","first-page":"291","DOI":"10.4081\/1154","volume":"51","author":"Xu","year":"2007","journal-title":"European journal of histochemistry : EJH","ISSN":"https:\/\/id.crossref.org\/issn\/1121-760X","issn-type":"print"}],"container-title":["Journal of Molecular Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/jme.bioscientifica.com\/view\/journals\/jme\/51\/1\/79.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/jme.bioscientifica.com\/downloadpdf\/journals\/jme\/51\/1\/79.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:52:00Z","timestamp":1784555520000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/jme\/article\/51\/1\/79-90\/11377"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,23]]},"references-count":40,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1530\/jme-12-0200","relation":{},"ISSN":["0952-5041","1479-6813"],"issn-type":[{"value":"0952-5041","type":"print"},{"value":"1479-6813","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,23]]}}}