{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T07:17:46Z","timestamp":1789543066264,"version":"build-2803163510"},"reference-count":47,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:p>\n                    Neonatal \u03b2 cells are considered developmentally immature and hence less glucose responsive. To study the acquisition of mature glucose responsiveness, we compared glucose-regulated redox state, insulin synthesis, and secretion of \u03b2 cells purified from neonatal or 10-week-old rats with their transcriptomes and proteomes measured by oligonucleotide and LC-MS\/MS profiling. Lower glucose responsiveness of neonatal \u03b2 cells was explained by two distinct properties: higher activity at low glucose and lower activity at high glucose. Basal hyperactivity was associated with higher NAD(P)H, a higher fraction of neonatal \u03b2 cells actively incorporating\n                    <jats:sup>3<\/jats:sup>\n                    H-tyrosine, and persistently increased insulin secretion below 5\u200amM glucose. Neonatal \u03b2 cells lacked the steep glucose-responsive NAD(P)H rise between 5 and 10\u200amM glucose characteristic for adult \u03b2 cells and accumulated less NAD(P)H at high glucose. They had twofold lower expression of malate\/aspartate-NADH shuttle and most glycolytic enzymes. Genome-wide profiling situated neonatal \u03b2 cells at a developmental crossroad: they showed advanced endocrine differentiation when specifically analyzed for their mRNA\/protein level of classical neuroendocrine markers. On the other hand, discrete neonatal \u03b2 cell subpopulations still expressed mRNAs\/proteins typical for developing\/proliferating tissues. One example, delta-like 1 homolog (DLK1) was used to investigate whether neonatal \u03b2 cells with basal hyperactivity corresponded to a more immature subset with high DLK1, but no association was found. In conclusion, the current study supports the importance of glycolytic NADH-shuttling in stimulus function coupling, presents basal hyperactivity as novel property of neonatal \u03b2 cells, and provides potential markers to recognize intercellular developmental differences in the endocrine pancreas.\n                  <\/jats:p>","DOI":"10.1530\/jme-13-0106","type":"journal-article","created":{"date-parts":[[2013,9,18]],"date-time":"2013-09-18T23:29:34Z","timestamp":1379546974000},"page":"11-28","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":38,"title":["Functional characteristics of neonatal rat \u03b2 cells with distinct markers"],"prefix":"10.1530","volume":"52","author":[{"given":"G 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