{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:19:28Z","timestamp":1784557168283,"version":"3.55.0"},"reference-count":59,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:p>\n                    EGR1 and PITX1 are transcription factors required for gonadotroph cell\n                    <jats:italic>Lhb<\/jats:italic>\n                    promoter activation. To determine changes in\n                    <jats:italic>Egr1<\/jats:italic>\n                    and\n                    <jats:italic>Pitx1<\/jats:italic>\n                    mRNA levels in central and peripheral pituitary stimulations, an\n                    <jats:italic>in vivo<\/jats:italic>\n                    model based on i.c.v. pulsatile (1 pulse\/0.5\u200ah over 2\u200ah) GnRH agonist (1.5\u200anM buserelin) or antagonist (2\u200anM antide) microinjections was used. The microinjections were given to ovariectomised and 17\u03b2-oestradiol (E\n                    <jats:sub>2<\/jats:sub>\n                    ) (3\u00d720\u200a\u03bcg), ERA (ESR1) agonist propyl pyrazole triol (PPT) (3\u00d70.5\u200amg), ERB (ESR2) agonist diarylpropionitrile (DPN) (3\u00d70.5\u200amg) s.c. pre-treated rats 30\u200amin after last pulse anterior pituitaries were excised. Relative mRNA expression was determined by quantitative RT-PCR (qRT-PCR). Results revealed a gene-specific response for GnRH and\/or oestrogenic stimulations\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Buserelin pulses enhanced\n                    <jats:italic>Egr1<\/jats:italic>\n                    expression by 66% in ovariectomised rats, whereas the oestradiol-supplemented+i.c.v. NaCl-microinjected group showed a 50% increase in\n                    <jats:italic>Egr1<\/jats:italic>\n                    mRNA expression. The oestrogenic signal was transmitted via ERA (ESR1) and ERB (ESR2) activation as administration of PPT and DPN resulted in 97 and 62%, respectively, elevation in\n                    <jats:italic>Egr1<\/jats:italic>\n                    mRNA expression. A synergistic action of GnRH agonist and 17\u03b2-oestradiol (E\n                    <jats:sub>2<\/jats:sub>\n                    ) stimulation of the\n                    <jats:italic>Egr1<\/jats:italic>\n                    gene transcription\n                    <jats:italic>in vivo<\/jats:italic>\n                    were found. GnRHR activity did not affect\n                    <jats:italic>Pitx1<\/jats:italic>\n                    mRNA expression; regardless of NaCl, buserelin or antide i.c.v. pulses, s.c. oestrogenic supplementation (with E\n                    <jats:sub>2<\/jats:sub>\n                    , PPT or DPN) consistently decreased (by \u221246, \u221248 and \u221241% respectively) the\n                    <jats:italic>Pitx1<\/jats:italic>\n                    mRNA in the anterior pituitary gland. Orchestrated\n                    <jats:italic>Egr1<\/jats:italic>\n                    and\n                    <jats:italic>Pitx1<\/jats:italic>\n                    activities depending on specific central and peripheral regulatory inputs could be responsible for physiologically variable\n                    <jats:italic>Lhb<\/jats:italic>\n                    gene promoter activation\n                    <jats:italic>in vivo<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1530\/jme-14-0092","type":"journal-article","created":{"date-parts":[[2014,9,26]],"date-time":"2014-09-26T03:17:56Z","timestamp":1411701476000},"page":"355-366","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":10,"title":["In vivo oestrogenic modulation of Egr1 and Pitx1 gene expression in female rat pituitary 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