{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T05:17:25Z","timestamp":1785043045128,"version":"3.55.0"},"reference-count":44,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:p>\n                    Neuromedin U (NMU) and neuromedin S (NMS) play inhibitory roles in the regulation of food intake and energy homeostasis in mammals. However, their functions are not clearly established in teleost fish. In the present study,\n                    <jats:italic>nmu<\/jats:italic>\n                    and\n                    <jats:italic>nms<\/jats:italic>\n                    homologs were identified in several fish species. Subsequently, their cDNA sequences were cloned from the orange-spotted grouper (\n                    <jats:italic>Epinephelus coioides<\/jats:italic>\n                    ). Sequence analysis showed that the orange-spotted grouper Nmu proprotein contains a 21-amino acid mature Nmu peptide (Nmu-21). The Nms proprotein lost the typical mature Nms peptide, but it retains a putative 34-amino acid peptide (Nmsrp).\n                    <jats:italic>In situ<\/jats:italic>\n                    hybridization revealed that\n                    <jats:italic>nmu<\/jats:italic>\n                    - and\n                    <jats:italic>nms<\/jats:italic>\n                    -expressing cells are mainly localized in the hypothalamic regions associated with appetite regulation. Food deprivation decreased the hypothalamic\n                    <jats:italic>nmu<\/jats:italic>\n                    mRNA levels but induced an increase of\n                    <jats:italic>nms<\/jats:italic>\n                    mRNA levels. Periprandial expression analysis showed that hypothalamic expression of\n                    <jats:italic>nmu<\/jats:italic>\n                    increased significantly at 3\u200ah post-feeding, while\n                    <jats:italic>nms<\/jats:italic>\n                    expression was elevated at the normal feeding time. I.p. injection of synthetic Nmu-21 peptide suppressed the hypothalamic neuropeptide y (\n                    <jats:italic>npy<\/jats:italic>\n                    ) expression, while Nmsrp administration significantly increased the expression of\n                    <jats:italic>npy<\/jats:italic>\n                    and\n                    <jats:italic>o<\/jats:italic>\n                    <jats:italic>rexin<\/jats:italic>\n                    in orange-spotted grouper. Furthermore, the mRNA levels of LH beta subunit (\n                    <jats:italic>lh<\/jats:italic>\n                    <jats:italic>\u03b2<\/jats:italic>\n                    ) and\n                    <jats:italic>gh<\/jats:italic>\n                    in the pituitary were significantly down-regulated after Nmu-21 peptide administration, while Nmsrp was able to significantly stimulate the expression of FSH beta subunit (\n                    <jats:italic>fsh<\/jats:italic>\n                    <jats:italic>\u03b2<\/jats:italic>\n                    ), prolactin (\n                    <jats:italic>prl<\/jats:italic>\n                    ), and somatolaction (\n                    <jats:italic>sl<\/jats:italic>\n                    ). Our results indicate that\n                    <jats:italic>nmu<\/jats:italic>\n                    and\n                    <jats:italic>nms<\/jats:italic>\n                    possess distinct neuroendocrine functions and pituitary functions in the orange spotted grouper.\n                  <\/jats:p>","DOI":"10.1530\/jme-15-0018","type":"journal-article","created":{"date-parts":[[2015,7,10]],"date-time":"2015-07-10T22:14:58Z","timestamp":1436566498000},"page":"95-106","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":13,"title":["Distinct functions of neuromedin u and neuromedin s in orange-spotted grouper"],"prefix":"10.1530","volume":"55","author":[{"given":"Shuisheng","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiongyu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binbin","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huapu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoran","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"2015110216260193000_55.2.95.1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21332"},{"key":"2015110216260193000_55.2.95.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2009.06.067"},{"key":"2015110216260193000_55.2.95.3","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0140157"},{"key":"2015110216260193000_55.2.95.4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00255.2009"},{"key":"2015110216260193000_55.2.95.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.05.028"},{"key":"2015110216260193000_55.2.95.6","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22171"},{"key":"2015110216260193000_55.2.95.7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-8206.2009.00667.x"},{"key":"2015110216260193000_55.2.95.8","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19991108)414:1<13::AID-CNE2>3.0.CO;2-R"},{"key":"2015110216260193000_55.2.95.9","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-0618(00)00063-6"},{"key":"2015110216260193000_55.2.95.10","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-0115(03)00144-7"},{"key":"2015110216260193000_55.2.95.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.04.103"},{"key":"2015110216260193000_55.2.95.12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M001546200"},{"key":"2015110216260193000_55.2.95.13","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2003.02079.x"},{"key":"2015110216260193000_55.2.95.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2003.10.098"},{"key":"2015110216260193000_55.2.95.15","doi-asserted-by":"publisher","DOI":"10.1038\/nm1106"},{"key":"2015110216260193000_55.2.95.16","doi-asserted-by":"publisher","DOI":"10.1038\/35017610"},{"key":"2015110216260193000_55.2.95.17","doi-asserted-by":"publisher","DOI":"10.1210\/en.2005-0107"},{"key":"2015110216260193000_55.2.95.18","doi-asserted-by":"publisher","DOI":"10.1210\/en.2002-220121"},{"key":"2015110216260193000_55.2.95.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2011.11.004"},{"key":"2015110216260193000_55.2.95.20","doi-asserted-by":"publisher","DOI":"10.1677\/joe.1.05948"},{"key":"2015110216260193000_55.2.95.21","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/5.2.150"},{"key":"2015110216260193000_55.2.95.22","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(00)00230-0"},{"key":"2015110216260193000_55.2.95.23","doi-asserted-by":"crossref","unstructured":"Malendowicz LK Ziolkowska A Rucinski M 2012 Neuromedins U and S involvement in the regulation of the hypothalamo-pituitary\u2013adrenal axis. 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