{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:19:56Z","timestamp":1784557196644,"version":"3.55.0"},"reference-count":53,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p>\n                    Stress exerts profound inhibitory effects on reproductive function by suppression of the pulsatile release of GnRH and therefore LH. Besides the corticotrophin-releasing factor (CRF), this effect also might be mediated via GABAergic signaling within the arcuate nucleus (ARC) since its inhibitory effects on LH pulses and increased activity during stress. In the present study, we investigated the role of endogenous GABAergic signaling within the ARC in stress-induced suppression of LH pulses. Ovariectomised oestradiol-replaced rats were implanted with bilateral and unilateral cannulae targeting toward the ARC and lateral cerebral ventricle respectively. Blood samples (25\u200a\u03bcl) were taken via chronically implanted cardiac catheters every 5\u200amin for 6\u200ah for measurement of LH pulses. Intra-ARC infusion of GABA\n                    <jats:sub>A<\/jats:sub>\n                    receptor antagonist, bicuculline (0.2\u200apmol in 200\u200anl artificial cerebrospinal fluid (aCSF) each side, three times at 20-min intervals) markedly attenuated the inhibitory effect of lipopolysaccharide (LPS; 25\u200a\u03bcg\/kg i.v.) but not restraint (1\u200ah) stress on pulsatile LH secretion. In contrast, restraint but not LPS stress-induced suppression of LH pulse frequency was reversed by intra-ARC administration of GABA\n                    <jats:sub>B<\/jats:sub>\n                    R antagonist, CGP-35348 (1.5\u200anmol in 200\u200anl aCSF each side, three times at 20-min intervals). Moreover, intra-ARC application of either bicuculline or CGP-35348 attenuated the inhibitory effect of CRF (1\u200anmol in 4\u200a\u03bcl aCSF, i.c.v.) on the LH pulses. These data indicate a pivotal and differential role of endogenous GABA\n                    <jats:sub>A<\/jats:sub>\n                    and GABA\n                    <jats:sub>B<\/jats:sub>\n                    signaling mechanisms in the ARC with respect to mediating immunological and psychological stress-induced suppression of the GnRH pulse generator respectively.\n                  <\/jats:p>","DOI":"10.1530\/jme-15-0084","type":"journal-article","created":{"date-parts":[[2015,5,21]],"date-time":"2015-05-21T21:56:05Z","timestamp":1432245365000},"page":"9-19","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":18,"title":["Stress-induced inhibition of LH pulses in female rats: role of GABA in arcuate nucleus"],"prefix":"10.1530","volume":"55","author":[{"given":"XiaoFeng","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ChengCheng","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin T","family":"O'Byrne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YuanShao","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"2015070718103686000_55.1.9.1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2005.01358.x"},{"key":"2015070718103686000_55.1.9.2","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1080001"},{"key":"2015070718103686000_55.1.9.3","doi-asserted-by":"crossref","unstructured":"Bowers G Cullinan WE Herman JP 1998 Region-specific regulation of glutamic acid decarboxylase (GAD) mRNA expression in central stress circuits. 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