{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T09:07:41Z","timestamp":1792919261149,"version":"4.5.0"},"reference-count":34,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:p>\n                    We recently showed that Xq26.3 microduplications cause X-linked acrogigantism (X-LAG). X-LAG patients mainly present with growth hormone and prolactin-secreting adenomas and share a minimal duplicated region containing at least four genes.\n                    <jats:italic>GPR101<\/jats:italic>\n                    was the only gene highly expressed in their pituitary lesions, but little is known about its expression patterns. In this work,\n                    <jats:italic>GPR101<\/jats:italic>\n                    transcripts were characterized in human tissues by 5\u2032-Rapid Amplification of cDNA Ends (RACE) and RNAseq, while the putative promoter was bioinformatically predicted. We investigated\n                    <jats:italic>GPR101<\/jats:italic>\n                    mRNA and protein expression by RT-quantitative PCR (qPCR), whole-mount\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization, and immunostaining, in human, rhesus monkey, rat and zebrafish. We identified four\n                    <jats:italic>GPR101<\/jats:italic>\n                    isoforms characterized by different 5\u2032-untranslated regions (UTRs) and a common 6.1kb long 3\u2032UTR.\n                    <jats:italic>GPR101<\/jats:italic>\n                    expression was very low or absent in almost all adult human tissues examined, except for specific brain regions. Strong GPR101 staining was observed in human fetal pituitary and during adolescence, whereas very weak\/absent expression was detected during childhood and adult life. In contrast to humans, adult monkey and rat pituitaries expressed GPR101, but in different cell types.\n                    <jats:italic>Gpr101<\/jats:italic>\n                    is expressed in the brain and pituitary during rat and zebrafish development; in rat pituitary,\n                    <jats:italic>Gpr101<\/jats:italic>\n                    is expressed only after birth and shows sexual dimorphism. This study shows that different\n                    <jats:italic>GPR101<\/jats:italic>\n                    transcripts exist and that the brain is the major site of\n                    <jats:italic>GPR101<\/jats:italic>\n                    expression across different species, although divergent species- and temporal-specific expression patterns are evident. These findings suggest an important role for GPR101 in brain and pituitary development and likely reflect the very different growth, development and maturation patterns among species.\n                  <\/jats:p>","DOI":"10.1530\/jme-16-0045","type":"journal-article","created":{"date-parts":[[2016,6,9]],"date-time":"2016-06-09T22:38:33Z","timestamp":1465511913000},"page":"97-111","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":45,"title":["Characterization of GPR101 transcript structure and expression patterns"],"prefix":"10.1530","volume":"57","author":[{"given":"Giampaolo","family":"Trivellin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Bjelobaba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian F","family":"Daly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darwin O","family":"Larco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonor","family":"Palmeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio R","family":"Faucz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Thiry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia F","family":"Leal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliya","family":"Rostomyan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha","family":"Quezado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie Helene","family":"Schernthaner-Reiter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marija M","family":"Janjic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Villa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T John","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanko S","family":"Stojilkovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Beckers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Feldman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constantine A","family":"Stratakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"2016071318300852000_57.2.97.1","doi-asserted-by":"publisher","DOI":"10.14310\/horm.2002.1195"},{"key":"2016071318300852000_57.2.97.2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01929"},{"key":"2016071318300852000_57.2.97.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.02.123"},{"key":"2016071318300852000_57.2.97.4","doi-asserted-by":"publisher","DOI":"10.1530\/ERC-15-0038"},{"key":"2016071318300852000_57.2.97.5","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.115.129320"},{"key":"2016071318300852000_57.2.97.6","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.anthro.28.1.109"},{"key":"2016071318300852000_57.2.97.7","doi-asserted-by":"publisher","DOI":"10.1038\/ng1789"},{"key":"2016071318300852000_57.2.97.8","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddt104"},{"key":"2016071318300852000_57.2.97.9","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-015-0797-8"},{"key":"2016071318300852000_57.2.97.10","doi-asserted-by":"publisher","DOI":"10.1530\/ERC-15-0478"},{"key":"2016071318300852000_57.2.97.11","doi-asserted-by":"publisher","DOI":"10.1530\/ERC-16-0082"},{"key":"2016071318300852000_57.2.97.12","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.115568"},{"key":"2016071318300852000_57.2.97.13","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-016-0328-1"},{"key":"2016071318300852000_57.2.97.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2014.01.022"},{"key":"2016071318300852000_57.2.97.15","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002030302"},{"key":"2016071318300852000_57.2.97.16","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(01)00651-5"},{"key":"2016071318300852000_57.2.97.17","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-1216"},{"key":"2016071318300852000_57.2.97.18","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1097\/MOP.0000000000000235","article-title":"Regulation of body growth","volume":"26","author":"Lui","year":"2015","journal-title":"Current Opinion in Pediatrics"},{"key":"2016071318300852000_57.2.97.19","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2199-9-102"},{"key":"2016071318300852000_57.2.97.20","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-48"},{"key":"2016071318300852000_57.2.97.21","doi-asserted-by":"crossref","unstructured":"Melmed S Kleinberg D Ho KEN 2011 Pituitary physiology and diagnostic evaluation. In Williams Textbook of Endocrinology, edn Twelfth Ed, pp 175\u2013228. Boston, MA, USA: Elsevier Inc.","DOI":"10.1016\/B978-1-4377-0324-5.00008-0"},{"key":"2016071318300852000_57.2.97.22","doi-asserted-by":"publisher","DOI":"10.1038\/tp.2013.130"},{"key":"2016071318300852000_57.2.97.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.12.026"},{"key":"2016071318300852000_57.2.97.24","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2006.01502.x"},{"key":"2016071318300852000_57.2.97.25","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2007.11.048"},{"key":"2016071318300852000_57.2.97.26","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.08.040"},{"key":"2016071318300852000_57.2.97.27","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2015-4366"},{"key":"2016071318300852000_57.2.97.28","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2026"},{"key":"2016071318300852000_57.2.97.29","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-547"},{"key":"2016071318300852000_57.2.97.30","unstructured":"Tariq M Cast AE Belmont JW Ware SM 2013 Identification of a novel cause of X-linked heterotaxy. In Proceedings of the American Society of Human Genetics 2013. Boston 22\u201326 October 2013. Program 227, Abstract 130122783. Bethesda, MD, USA: American Society of Human Genetics. (available at: http:\/\/www.ashg.org\/2013meeting\/abstracts\/fulltext\/search_page-04.shtml)"},{"key":"2016071318300852000_57.2.97.31","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.514"},{"key":"2016071318300852000_57.2.97.32","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01363-06"},{"key":"2016071318300852000_57.2.97.33","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.6A7134.2007"},{"key":"2016071318300852000_57.2.97.34","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1408028"}],"container-title":["Journal of Molecular Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/jme.bioscientifica.com\/view\/journals\/jme\/57\/2\/97.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/jme.bioscientifica.com\/downloadpdf\/journals\/jme\/57\/2\/97.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:52:57Z","timestamp":1784555577000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/jme\/article\/57\/2\/97-111\/11634"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8]]},"references-count":34,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1530\/jme-16-0045","relation":{},"ISSN":["0952-5041","1479-6813"],"issn-type":[{"value":"0952-5041","type":"print"},{"value":"1479-6813","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8]]}}}