{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T02:04:38Z","timestamp":1792029878696,"version":"4.4.0"},"reference-count":48,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:p>\n                    Melatonin, synthesized primarily by the pineal gland, is a neuroendocrine hormone with high membrane permeability. The vascular effects of melatonin, including vasoconstriction and vasodilation, have been demonstrated in numerous studies. However, the mechanisms underlying these effects are not fully understood. Large-conductance Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -activated K\n                    <jats:sup>+<\/jats:sup>\n                    (BK\n                    <jats:sub>Ca<\/jats:sub>\n                    ) channels are expressed broadly on smooth muscle cells and play an important role in vascular tone regulation. This study explored the mechanisms of myocyte BK\n                    <jats:sub>Ca<\/jats:sub>\n                    channels and endothelial factors underlying the action of melatonin on the mesenteric arteries (MAs). Vascular contractility and patch-clamp studies were performed on myocytes of MAs from Wistar rats. Melatonin induced significant vasodilation on MAs. In the presence of\n                    <jats:italic>N<\/jats:italic>\n                    <jats:sup>\u03c9<\/jats:sup>\n                    -nitro-\n                    <jats:sc>l<\/jats:sc>\n                    -arginine methyl ester (\n                    <jats:sc>l<\/jats:sc>\n                    -NAME), a potent endothelial oxide synthase (eNOS) inhibitor, melatonin elicited concentration-dependent relaxation, with lowered pIC\n                    <jats:sub>50<\/jats:sub>\n                    . The effect of melatonin was significantly attenuated in the presence of BK\n                    <jats:sub>Ca<\/jats:sub>\n                    channel blocker iberiotoxin or MT1\/MT2 receptor antagonist luzindole in both (+)\n                    <jats:sc>l<\/jats:sc>\n                    -NAME and (\u2212)\n                    <jats:sc>l<\/jats:sc>\n                    -NAME groups. In the (+)\n                    <jats:sc>l<\/jats:sc>\n                    -NAME group, iberiotoxin caused a parallel rightward shift of the melatonin concentration\u2013relaxation curve, with pIC\n                    <jats:sub>50<\/jats:sub>\n                    lower than that of luzindole. Both inside-out and cell-attached patch-clamp recordings showed that melatonin significantly increased the open probability, mean open time and voltage sensitivity of BK\n                    <jats:sub>Ca<\/jats:sub>\n                    channels. In a cell-attached patch-clamp configuration, the melatonin-induced enhancement of BK\n                    <jats:sub>Ca<\/jats:sub>\n                    channel activity was significantly suppressed by luzindole. These findings indicate that in addition to the activation of eNOS, melatonin-induced vasorelaxation of MAs is partially attributable to its direct (passing through the cell membrane) and indirect (via MT1\/MT2 receptors) activation of the BK\n                    <jats:sub>Ca<\/jats:sub>\n                    channels on mesenteric arterial myocytes.\n                  <\/jats:p>","DOI":"10.1530\/jme-17-0028","type":"journal-article","created":{"date-parts":[[2017,7,4]],"date-time":"2017-07-04T20:20:17Z","timestamp":1499199617000},"page":"219-233","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":42,"title":["Melatonin mediates vasodilation through both direct and indirect activation of BKCa channels"],"prefix":"10.1530","volume":"59","author":[{"given":"T","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Exercise Physiology, Beijing Sport University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Exercise Physiology, Beijing Sport University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Exercise Physiology, Beijing Sport University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Exercise Physiology, Beijing Sport University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Exercise Physiology, Beijing Sport University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Exercise Physiology, 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