{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T22:49:16Z","timestamp":1788562156732,"version":"build-2803163510"},"reference-count":18,"publisher":"Bioscientifica","issue":"4","content-domain":{"domain":["jme.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:p>\n                    Insulin-regulated aminopeptidase (IRAP) co-localizes with the glucose transporter 4 (GLUT4) in GLUT4 storage vesicles (GSV) in insulin-responsive cells. In response to insulin, IRAP is the only transmembrane enzyme known to translocate together with GLUT4 to the plasma membrane in adipocytes and muscle cells. Although the intracellular region of IRAP is associated with GLUT4 vesicle trafficking, the role of the aminopeptidase activity in insulin-responsive cells has not been elucidated. The aim of this study was to investigate whether the inhibition of the aminopeptidase activity of IRAP facilitates glucose uptake in insulin-responsive cells. In both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    studies, inhibition of IRAP aminopeptidase activity with the specific inhibitor, HFI-419, did not modulate glucose uptake. IRAP inhibition in the L6GLUT4myc cell line did not alter glucose uptake in both basal and insulin-stimulated state. In keeping with these results, HFI419 did not affect peripheral, whole-body glucose handling after an oral glucose challenge, neither in normal rats nor in the streptozotocin (STZ)-induced experimental rat model of diabetes mellitus (DM). Therefore, acute inhibition of IRAP aminopeptidase activity does not affect glucose homeostasis.\n                  <\/jats:p>","DOI":"10.1530\/jme-17-0033","type":"journal-article","created":{"date-parts":[[2017,3,29]],"date-time":"2017-03-29T20:20:14Z","timestamp":1490818814000},"page":"193-198","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":5,"title":["Insulin-regulated aminopeptidase inhibitors do not alter glucose handling in normal and diabetic rats"],"prefix":"10.1530","volume":"58","author":[{"given":"Anthony L","family":"Albiston","sequence":"first","affiliation":[{"name":"1College of Health and BiomedicineVictoria University St Albans, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauricio","family":"Cacador","sequence":"additional","affiliation":[{"name":"1College of Health and BiomedicineVictoria University St Albans, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puspha","family":"Sinnayah","sequence":"additional","affiliation":[{"name":"1College of Health and BiomedicineVictoria University St Albans, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peta","family":"Burns","sequence":"additional","affiliation":[{"name":"2Department of PhysiologyBiomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siew Yeen","family":"Chai","sequence":"additional","affiliation":[{"name":"2Department of PhysiologyBiomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"2017050217101461000_58.4.193.1","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/j.pharmthera.2007.07.006","article-title":"Therapeutic targeting of insulin-regulated aminopeptidase: heads and tails?","volume":"116","author":"Albiston","year":"2007","journal-title":"Pharmacology and Therapeutics"},{"key":"2017050217101461000_58.4.193.2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.08-112227"},{"key":"2017050217101461000_58.4.193.3","doi-asserted-by":"publisher","DOI":"10.1038\/nrm782"},{"key":"2017050217101461000_58.4.193.4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.04659.x"},{"key":"2017050217101461000_58.4.193.5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2008.06347.x"},{"key":"2017050217101461000_58.4.193.6","first-page":"E600","article-title":"Insulin stimulates cell surface aminopeptidase activity toward vasopressin in adipocytes","volume":"272","author":"Herbst","year":"1997","journal-title":"American Journal of Physiology"},{"key":"2017050217101461000_58.4.193.7","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.40.23612"},{"key":"2017050217101461000_58.4.193.8","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2003.01852.x"},{"key":"2017050217101461000_58.4.193.9","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2003.01852.x"},{"key":"2017050217101461000_58.4.193.10","doi-asserted-by":"publisher","first-page":"534","DOI":"10.1016\/j.bbrc.2016.10.056","article-title":"Staurosporine as an agonist for induction of GLUT4 translocation, identified by a pH-sensitive fluorescent IRAP-mOrange2 probe","volume":"480","author":"Li","year":"2016","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"2017050217101461000_58.4.193.11","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.13.4611-4626.2003"},{"key":"2017050217101461000_58.4.193.12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.6.3328"},{"key":"2017050217101461000_58.4.193.13","first-page":"BR195","article-title":"Placental leucine aminopeptidase might regulate the effects of oxytocin with resolution in endothelial cells.","volume":"11","author":"Sano","year":"2005","journal-title":"Medical Science Monitor"},{"key":"2017050217101461000_58.4.193.14","doi-asserted-by":"publisher","DOI":"10.1038\/ni1208"},{"key":"2017050217101461000_58.4.193.15","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(92)90007-J"},{"key":"2017050217101461000_58.4.193.16","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.43.30864"},{"key":"2017050217101461000_58.4.193.17","doi-asserted-by":"publisher","unstructured":"Yamamoto N Ueda M Sato T Kawasaki K Sawada K Kawabata K Ashida H 2011 Measurement of glucose uptake in cultured cells. 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